scholarly journals Highly Sensitive Detection and Localization of Maternally Acquired Human Cytomegalovirus in Placental Tissue by In Situ Polymerase Chain Reaction

2005 ◽  
Vol 192 (4) ◽  
pp. 650-657 ◽  
Author(s):  
Daniel E. Trincado ◽  
Sian C. Munro ◽  
Catherine Camaris ◽  
William D. Rawlinson
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Cytomegalovirus ◽  
Placental Tissue ◽  
Sensitive Detection ◽  
Chain Reaction ◽  
Highly Sensitive ◽  
Polymerase Chain ◽  
Highly Sensitive Detection ◽  
Detection And Localization

scholarly journals Rapid and Highly Sensitive Detection by a Real-time Polymerase Chain Reaction Using a Chip Coated with Its Reagents

2014 ◽  
Vol 30 (5) ◽  
pp. 569-574 ◽  
Author(s):  
Shunsuke FURUTANI ◽  
Nahoko NARUISHI ◽  
Masato SAITO ◽  
Eiichi TAMIYA ◽  
Yusuke FUCHIWAKI ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Sensitive Detection ◽  
Chain Reaction ◽  
Highly Sensitive ◽  
Polymerase Chain ◽  
Highly Sensitive Detection

High performance Nanogold™-in situ hybridzation and its use in the detection of hybridized and PCR-amplified microscopical preparations

1996 ◽  
Vol 54 ◽  
pp. 896-897
Author(s):  
G. W. Hacker ◽  
I. Zehbe ◽  
J. Hainfeld ◽  
A.-H. Graf ◽  
C. Hauser-Kronberger ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Messenger Rna ◽  
High Performance ◽  
Detection System ◽  
Direct Methods ◽  
Genetic Alterations ◽  
Chain Reaction ◽  
Protein Encoding ◽  
Polymerase Chain

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.

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