The Discovery and Characterization of Transposable Elements. Genes, Cells and Organisms. Barbara McClintock

1988 ◽  
Vol 63 (4) ◽  
pp. 458-459
Author(s):  
Bentley Glass
2012 ◽  
Vol 10 (4) ◽  
pp. 3-13
Author(s):  

The paper describes the early part of Barbara McClintock`s work on DNA transposons in maize, in which she discovered the Ac-Ds family of mobile "controlling elements". An account is first given of the cytology of the system that was used to generate intact chromosomes having "sticky" (broken) ends. Cytogenetical aspects of the chromatid and chromosome breakage-fusion-bridge cycles, deriving from breakage, are then described, which leads on to the way in which variegation in phenotypes of the maize kernels could be "read" in terms of chromosome breakage. The "genetic earthquake" event of 1944, triggered by introducing broken chromosomes into a zygote from both parents, lead to the discovery of Ds and Ac. Finding mobility of Ds from one chromosomal location to another was pure serendipity: the transposition showed itself while experiments were being undertaken to accurately map Ds. A similar chance observation revealed transposition of Ac as well, and then the relationship between the two elements was elucidated in terms of their autonomous and non-autonomous nature.


BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Vanessa Carvalho Cayres Pamponét ◽  
Margarete Magalhães Souza ◽  
Gonçalo Santos Silva ◽  
Fabienne Micheli ◽  
Cláusio Antônio Ferreira de Melo ◽  
...  

2002 ◽  
Vol 68 (5) ◽  
pp. 2307-2315 ◽  
Author(s):  
Masahiro Sota ◽  
Masahiro Endo ◽  
Keiji Nitta ◽  
Haruhiko Kawasaki ◽  
Masataka Tsuda

ABSTRACT The two haloacetate dehalogenase genes, dehH1 and dehH2, on the 65-kb plasmid pUO1 from Delftia acidovorans strain B were found to be located on transposable elements. The dehH2 gene was carried on an 8.9-kb class I composite transposon (TnHad1) that was flanked by two directly repeated copies of IS1071, IS1071L and IS1071R. The dehH1 gene was also flanked by IS1071L and a truncated version of IS1071 (IS1071N). TnHad1, dehH1, and IS1071N were located on a 15.6-kb class II transposon (TnHad2) whose terminal inverted repeats and res site showed high homology with those of the Tn21-related transposons. TnHad2 was defective in transposition because of its lacking the transposase and resolvase genes. TnHad2 could transpose when the Tn21-encoded transposase and resolvase were supplied in trans. These results demonstrated that Tn Had2 is a defective Tn21-related transposon carrying another class I catabolic transposon.


PLoS ONE ◽  
2012 ◽  
Vol 7 (8) ◽  
pp. e40197 ◽  
Author(s):  
Jessy Labbé ◽  
Claude Murat ◽  
Emmanuelle Morin ◽  
Gerald A. Tuskan ◽  
François Le Tacon ◽  
...  

2019 ◽  
Vol 8 (3) ◽  
pp. 77-85
Author(s):  
Mahmoud A Gaber ◽  
Elsayed E Wagih ◽  
Mohamed R. A Shehata ◽  
Magda M Fahmy ◽  
Hala Abdel Wahab

Botrytis cinerea is a necrotrophic plant pathogen that causes plenty of crop losses in Egypt and worldwide. Fifteen isolates of B. cinerea were collected from cabbage, pepper and lettuce grown in different locations in Egypt and subjected to investigation. Diversity in phenotypic, pathological and molecular characteristics was detected among isolates, leading to categorising them into four different groups. Molecular variation was demonstrated in all isolates by transposable elements (TEs) analyses. Four TE types, based on the presence or absence of two transposable elements, boty and flipper, were recognised among B. cinerea isolates in which transposa type (having both TE, boty + flipper) was predominant (40%), while only boty and only flipper types appeared with distribution values of 26.7 and 20%, respectively and vacuma type (Lacking both TEs) showed the lowest distribution value (13.3%). Furthermore, vacuma population demonstrated the lowest potential comparing to others. A correlation was found between TE type and virulence level of isolate, but no impact of TE type was observed on phenotypic characteristics of B. cinerea. The present study revealed a correlation between the TE type and the isolate virulence, but no correlation was observed between phenotypic characteristic/sensitivity to fenhexamid and TE type


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