Nuclear Transplantation in Amphibia and the Importance of Stable Nuclear Changes in Promoting Cellular Differentiation

1963 ◽  
Vol 38 (1) ◽  
pp. 54-78 ◽  
Author(s):  
J. B. Gurdon
Keyword(s):  
Cellular Differentiation ◽  
Nuclear Transplantation ◽  
Nuclear Changes

The Developmental Capacity of Nuclei taken from Intestinal Epithelium Cells of Feeding Tadpoles

Development ◽  
1962 ◽  
Vol 10 (4) ◽  
pp. 622-640 ◽  
Author(s):  
J. B. Gurdon
Keyword(s):  
Genetic Information ◽  
Cellular Differentiation ◽  
Cell Types ◽  
Nuclear Transplantation ◽  
Differentiated Cells ◽  
Developmental Capacity ◽  
Nuclear Changes ◽  
Different Cell Types ◽  
Epithelium Cells ◽  
Saline Medium

An important problem in embryology is whether the differentiation of cells depends upon a stable restriction of the genetic information contained in their nuclei. The technique of nuclear transplantation has shown to what extent the nuclei of differentiating cells can promote the formation of different cell types (e.g. King & Briggs, 1956; Gurdon, 1960c). Yet no experiments have so far been published on the transplantation of nuclei from fully differentiated normal cells. This is partly because it is difficult to obtain meaningful results from such experiments. The small amount of cytoplasm in differentiated cells renders their nuclei susceptible to damage through exposure to the saline medium, and this makes it difficult to assess the significance of the abnormalities resulting from their transplantation. It is, however, very desirable to know the developmental capacity of such nuclei, since any nuclear changes which are necessarily involved in cellular differentiation must have already taken place in cells of this kind.

scholarly journals The Developmental Capacity of Nuclei Taken from Differentiating Endoderm Cells of Xenopus laevis

Development ◽  
1960 ◽  
Vol 8 (4) ◽  
pp. 505-526
Author(s):  
J. B. Gurdon
Keyword(s):  
Xenopus Laevis ◽  
Rana Pipiens ◽  
Nuclear Transplantation ◽  
Blastula Stage ◽  
Developmental Capacity ◽  
Nuclear Changes ◽  
Nuclear Differentiation ◽  
Development Proceeds ◽  
Transplantation Experiments ◽  
Different Parts

An important question concerning embryonic differentiation is whether the nuclei of somatic cells in different parts of an embryo come to differ genetically from each other during development. It has become possible to investigate this matter since King & Briggs (1955) have shown that nuclear transplantation is a satisfactory technique for testing the developmental potentialities of embryonic nuclei. These authors (1957, 1960) have used Rana pipiens for transplantation experiments with endoderm nuclei, and have found that these nuclei become progressively limited in their developmental capacity after the late blastula stage. This paper describes some similar experiments carried out with endoderm nuclei of Xenopus laevis. The general conclusion that nuclei change as development proceeds is confirmed; there are, however, considerable differences between Rana and Xenopus in the rate and time of onset of nuclear changes. These differences make it easier to understand the significance of nuclear differentiation during embryonic development.

Oligomeric procyanidins as inductors of cellular differentiation of epidermal keratinocytes

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
E Kisseih Oppong Bekoe ◽  
F Petereit ◽  
C Agyare ◽  
M Lechtenberg ◽  
A Hensel
Keyword(s):  
Cellular Differentiation ◽  
Epidermal Keratinocytes

Researches Concerning the Phenomena at the Interface for the Sintered Compacts of Titan-Hydroxyapatite

2013 ◽  
Vol 856 ◽  
pp. 164-168 ◽  
Author(s):  
Diana Irinel Băilă
Keyword(s):  
Specific Surface ◽  
Bone Tissue ◽  
Contact Zone ◽  
Cellular Differentiation ◽  
Selective Laser Sintering ◽  
Important Process ◽  
Sintering Process ◽  
Cellular Processes ◽  
Hydroxyapatite Powder ◽  
Ti Powder

The behaviour of metallic powders is very important for manufacturing parts and for realize the prototypes. Manufacturing of the orthopaedic implants by selective laser sintering process can be used in medicine. The powders necessary for SLS process are biomaterials who must assure a good osteointegration of endobone implants like the Ti powder (Ti-6Al-4V) and the hydroxyapatite powder. The conditioned powders is a very important process and is necessary in SLS to obtain medical prothesis and influence the final properties of prothesis. Materials must present a gradient for porosity and one for composition (presents of a bioactive phase in titan matrix). The exterior porosity, in the contact zones with the bone tissue, favors the incipient cellular processes (the adhesion and attachment of osteoblastes, proliferation and cellular differentiation and nucleation of mineral bone). The pores must be open and with a specific surface more great. Its preferred the exterior intercommunicating porosity. The presence of phosphates of calcium in the contact zone of the implant with bone favors the mineral kernel on bone. The report Ca/P of phosphates mineral bone has an ascending evolution towards 10/6 according to hydroxyapatite.

scholarly journals 1116-22 Development of a transgenic mouse model for imaging of cellular differentiation into cardiomyocyte

2004 ◽  
Vol 43 (5) ◽  
pp. A24-A25
Author(s):  
Jin Chul Paeng ◽  
Dong Soo Lee ◽  
Joo Hyun Kang ◽  
June-Key Chung ◽  
Myung Chul Lee
Keyword(s):  
Mouse Model ◽  
Transgenic Mouse ◽  
Cellular Differentiation ◽  
Transgenic Mouse Model

scholarly journals SUGAR-seq enables simultaneous detection of glycans, epitopes, and the transcriptome in single cells

2021 ◽  
Vol 7 (8) ◽  
pp. eabe3610
Author(s):  
Conor J. Kearney ◽  
Stephin J. Vervoort ◽  
Kelly M. Ramsbottom ◽  
Izabela Todorovski ◽  
Emily J. Lelliott ◽  
...  
Keyword(s):  
Single Cell ◽  
Posttranslational Modification ◽  
Cellular Differentiation ◽  
Single Cells ◽  
Simultaneous Detection ◽  
Surface Protein ◽  
Rna Seq ◽  
Cell Level ◽  
Simultaneous Integration ◽  
Unique Surface

Multimodal single-cell RNA sequencing enables the precise mapping of transcriptional and phenotypic features of cellular differentiation states but does not allow for simultaneous integration of critical posttranslational modification data. Here, we describe SUrface-protein Glycan And RNA-seq (SUGAR-seq), a method that enables detection and analysis of N-linked glycosylation, extracellular epitopes, and the transcriptome at the single-cell level. Integrated SUGAR-seq and glycoproteome analysis identified tumor-infiltrating T cells with unique surface glycan properties that report their epigenetic and functional state.

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