scholarly journals The Western and Eastern Roots of the Saami—the Story of Genetic “Outliers” Told by Mitochondrial DNA and Y Chromosomes

2004 ◽  
Vol 74 (4) ◽  
pp. 661-682 ◽  
Author(s):  
Kristiina Tambets ◽  
Siiri Rootsi ◽  
Toomas Kivisild ◽  
Hela Help ◽  
Piia Serk ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Y Chromosomes

Differentiation of Mitochondrial DNA and Y Chromosomes in Russian Populations

Human Biology ◽  
2004 ◽  
Vol 76 (6) ◽  
pp. 877-900 ◽  
Author(s):  
Boris Malyarchuk ◽  
Miroslava Derenko ◽  
Tomasz Grzybowski ◽  
Arina Lunkina ◽  
Jakub Czarny ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Y Chromosomes

scholarly journals Contrasting Signatures of Population Growth for Mitochondrial DNA and Y Chromosomes among Human Populations in Africa

2007 ◽  
Vol 25 (3) ◽  
pp. 517-525 ◽  
Author(s):  
Maya Metni Pilkington ◽  
Jason A. Wilder ◽  
Fernando L. Mendez ◽  
Murray P. Cox ◽  
August Woerner ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Population Growth ◽  
Human Populations ◽  
Y Chromosomes

scholarly journals The evolutionary history of Neanderthal and Denisovan Y chromosomes

Science ◽  
2020 ◽  
Vol 369 (6511) ◽  
pp. 1653-1656 ◽  
Author(s):  
Martin Petr ◽  
Mateja Hajdinjak ◽  
Qiaomei Fu ◽  
Elena Essel ◽  
Hélène Rougier ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Evolutionary History ◽  
Genetic Load ◽  
Modern Human ◽  
Gene Pools ◽  
Chromosomal Gene ◽  
Human History ◽  
Effective Population ◽  
Y Chromosomes ◽  
History Of

Ancient DNA has provided new insights into many aspects of human history. However, we lack comprehensive studies of the Y chromosomes of Denisovans and Neanderthals because the majority of specimens that have been sequenced to sufficient coverage are female. Sequencing Y chromosomes from two Denisovans and three Neanderthals shows that the Y chromosomes of Denisovans split around 700 thousand years ago from a lineage shared by Neanderthals and modern human Y chromosomes, which diverged from each other around 370 thousand years ago. The phylogenetic relationships of archaic and modern human Y chromosomes differ from the population relationships inferred from the autosomal genomes and mirror mitochondrial DNA phylogenies, indicating replacement of both the mitochondrial and Y chromosomal gene pools in late Neanderthals. This replacement is plausible if the low effective population size of Neanderthals resulted in an increased genetic load in Neanderthals relative to modern humans.

scholarly journals The evolutionary history of Neandertal and Denisovan Y chromosomes

2020 ◽  
Author(s):  
Martin Petr ◽  
Mateja Hajdinjak ◽  
Qiaomei Fu ◽  
Elena Essel ◽  
Hélène Rougier ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Evolutionary History ◽  
Genetic Load ◽  
Modern Human ◽  
Gene Pools ◽  
Human History ◽  
Effective Population ◽  
Modern Humans ◽  
Y Chromosomes ◽  
History Of

AbstractAncient DNA has allowed the study of various aspects of human history in unprecedented detail. However, because the majority of archaic human specimens preserved well enough for genome sequencing have been female, comprehensive studies of Y chromosomes of Denisovans and Neandertals have not yet been possible. Here we present sequences of the first Denisovan Y chromosomes (Denisova 4 and Denisova 8), as well as the Y chromosomes of three late Neandertals (Spy 94a, Mezmaiskaya 2 and El Sidrón 1253). We find that the Denisovan Y chromosomes split around 700 thousand years ago (kya) from a lineage shared by Neandertal and modern human Y chromosomes, which diverged from each other around 370 kya. The phylogenetic relationships of archaic and modern human Y chromosomes therefore differ from population relationships inferred from their autosomal genomes, and mirror the relationships observed on the level of mitochondrial DNA. This provides strong evidence that gene flow from an early lineage related to modern humans resulted in the replacement of both the mitochondrial and Y chromosomal gene pools in late Neandertals. Although unlikely under neutrality, we show that this replacement is plausible if the low effective population size of Neandertals resulted in an increased genetic load in their Y chromosomes and mitochondrial DNA relative to modern humans.

scholarly journals Discordant Phylogeographic Patterns Between the Y Chromosome and Mitochondrial DNA in the House Mouse: Selection on the Y Chromosome?

Genetics ◽  
1997 ◽  
Vol 146 (3) ◽  
pp. 1019-1034 ◽  
Author(s):  
Stéphane Boissinot ◽  
Pierre Boursot
Keyword(s):  
Mitochondrial Dna ◽  
Molecular Evolution ◽  
Y Chromosome ◽  
Mus Musculus ◽  
Divergence Time ◽  
Ancestral Polymorphism ◽  
Base Pairs ◽  
Y Chromosomes ◽  
Rapid Spread ◽  
Mtdna Lineages

We have compared patterns of geographic variation and molecular divergence of mitochondrial DNA (mtDNA) and Y chromosome over the range of the different subspecies of Mus musculus. MtDNA was typed for 305 nucleotides in the control region, the Y chromosome for 834 base pairs (bp) in Zfy introns and 242 bp in Sry, a Zfy2 18-bp deletion, and two microsatellites. Apparent discrepancies exist between the distributions of the lineages of mtDNA and of the two major Y-chromosome lineages thus defined: some subspecies share the same mtDNA lineage but have different Y-chromosome lineages or vice versa. One microsatellite reveals a geographically clustered variation inside the distribution of each Y-chromosome lineage, showing that new Y-chromosome variants can rapidly spread locally. The two major Y-chromosome lineages have a divergence time only about one fourth of that between mtDNA lineages. Although this recent coalescence of the Y chromosomes between subspecies could partly be due to a lower ancestral polymorphism of the Y chromosome, it suggests that secondary introgression after the radiation of the subspecies might have occurred. There is evidence that the differentiation of the Y-chromosome lineages contributes to partial reproductive isolation between subspecies, and patterns of molecular evolution suggest that selection has played a role in the rapid spread across subspecies.

Evaluation of the dark field method for large association of spread DNA

1978 ◽  
Vol 36 (2) ◽  
pp. 190-191
Author(s):  
Douglas C. Barker
Keyword(s):  
Electron Microscopy ◽  
Molecular Weight ◽  
Mitochondrial Dna ◽  
Extraction Method ◽  
Field Method ◽  
Dark Field ◽  
Kinetoplast Dna ◽  
Field Electron ◽  
Field Electron Microscopy ◽  
Dark Field Electron

A number of satisfactory methods are available for the electron microscopy of nicleic acids. These methods concentrated on fragments of nuclear, viral and mitochondrial DNA less than 50 megadaltons, on denaturation and heteroduplex mapping (Davies et al 1971) or on the interaction between proteins and DNA (Brack and Delain 1975). Less attention has been paid to the experimental criteria necessary for spreading and visualisation by dark field electron microscopy of large intact issociations of DNA. This communication will report on those criteria in relation to the ultrastructure of the (approx. 1 x 10-14g) DNA component of the kinetoplast from Trypanosomes. An extraction method has been developed to eliminate native endonucleases and nuclear contamination and to isolate the kinetoplast DNA (KDNA) as a compact network of high molecular weight. In collaboration with Dr. Ch. Brack (Basel [nstitute of Immunology), we studied the conditions necessary to prepare this KDNA Tor dark field electron microscopy using the microdrop spreading technique.

Properties of Isolated Mitochondria of Agaricus Bisporus: Their Relationship to Dormancy and the Germination of Spores

1973 ◽  
Vol 31 ◽  
pp. 458-459
Author(s):  
K. S. McCarty ◽  
R. F. Weave ◽  
L. Kemper ◽  
F. S. Vogel
Keyword(s):  
Mitochondrial Dna ◽  
Ethidium Bromide ◽  
Microscopic Examination ◽  
Agaricus Bisporus ◽  
Osmotic Shock ◽  
Electron Microscopic Examination ◽  
Electron Microscopic ◽  
Isolated Mitochondria ◽  
Dna Strands ◽  
Cytoplasmic Ribosomes

During the prodromal stages of sporulation in the Basidiomycete, Agaricus bisporus, mitochondria accumulate in the basidial cells, zygotes, in the gill tissues prior to entry of these mitochondria, together with two haploid nuclei and cytoplasmic ribosomes, into the exospores. The mitochondria contain prominent loci of DNA [Fig. 1]. A modified Kleinschmidt spread technique1 has been used to evaluate the DNA strands from purified whole mitochondria released by osmotic shock, mitochondrial DNA purified on CsCl gradients [density = 1.698 gms/cc], and DNA purified on ethidium bromide CsCl gradients. The DNA appeared as linear strands up to 25 u in length and circular forms 2.2-5.2 u in circumference. In specimens prepared by osmotic shock, many strands of DNA are apparently attached to membrane fragments [Fig. 2]. When mitochondria were ruptured in hypotonic sucrose and then fixed in glutaraldehyde, the ribosomes were released for electron microscopic examination.

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