The Phytochrome Gene Family in the Stellaria longipes Complex

2003 ◽  
Vol 164 (5) ◽  
pp. 657-673 ◽  
Author(s):  
Wen‐Ze Li ◽  
C. C. Chinnappa
Keyword(s):  
Gene Family ◽  
Stellaria Longipes ◽  
Phytochrome Gene

scholarly journals The phytochrome gene family in tomato (Solanum lycopersicum L.)

1997 ◽  
Vol 20 (6) ◽  
pp. 672-677 ◽  
Author(s):  
L. H. PRATT ◽  
M.-M. CORDONNIER-PRATT ◽  
P. M. KELMENSON ◽  
G. I. LAZAROVA ◽  
T. KUBOTA ◽  
...  
Keyword(s):  
Gene Family ◽  
Solanum Lycopersicum ◽  
Solanum Lycopersicum L ◽  
Phytochrome Gene

Characterization and expression of the phytochrome gene family in the moss Ceratodon purpureus

2002 ◽  
Vol 13 (1) ◽  
pp. 51-61 ◽  
Author(s):  
Konstantinos Pasentsis ◽  
Nicola Paulo ◽  
Patricia Algarra ◽  
Peter Dittrich ◽  
Fritz Thümmler
Keyword(s):  
Gene Family ◽  
Ceratodon Purpureus ◽  
Phytochrome Gene

Divergence of the phytochrome gene family predates angiosperm evolution and suggests thatSelaginella andEquisetum arose prior toPsilotum

1995 ◽  
Vol 41 (3) ◽  
pp. 329-337 ◽  
Author(s):  
H. �. Kolukisaoglu ◽  
S. Marx ◽  
C. Wiegmann ◽  
S. Hanelt ◽  
H. A. W. Schneider-Poetsch
Keyword(s):  
Gene Family ◽  
Angiosperm Evolution ◽  
Phytochrome Gene

Analysis of the phytochrome gene family in Ceratodon purpureus by gene targeting reveals the primary phytochrome responsible for photo- and polarotropism

Planta ◽  
2009 ◽  
Vol 230 (1) ◽  
pp. 27-37 ◽  
Author(s):  
Franz Mittmann ◽  
Sven Dienstbach ◽  
Andrea Weisert ◽  
Christoph Forreiter
Keyword(s):  
Gene Family ◽  
Gene Targeting ◽  
Ceratodon Purpureus ◽  
Phytochrome Gene

scholarly journals Multiplex CRISPR-Cas9 mutagenesis of the phytochrome gene family in Physcomitrium (Physcomitrella) patens

2020 ◽  
Author(s):  
Silvia Trogu ◽  
Anna Lena Ermert ◽  
Fabian Stahl ◽  
Fabien Nogué ◽  
Tanja Gans ◽  
...  
Keyword(s):  
Gene Family ◽  
Lower Order ◽  
Physcomitrella Patens ◽  
Gene Families ◽  
Mutant Library ◽  
Highly Efficient ◽  
Phytochrome Gene ◽  
Efficient Screening ◽  
Mutant Collection ◽  
Proof Of Principle

Key message We mutated all seven Physcomitrium (Physcomitrella) patens phytochrome genes using highly-efficient CRISPR-Cas9 procedures. We thereby identified phy5a as the phytochrome primarily responsible for inhibiting gravitropism, proving the utility of the mutant library. Abstract The CRISPR-Cas9 system is a powerful tool for genome editing. Here we report highly-efficient multiplex CRISPR-Cas9 editing of the seven-member phytochrome gene family in the model bryophyte Physcomitrium (Physcomitrella) patens. Based on the co-delivery of an improved Cas9 plasmid with multiple sgRNA plasmids and an efficient screening procedure to identify high-order multiple mutants prior to sequencing, we demonstrate successful targeting of all seven PHY genes in a single transfection. We investigated further aspects of the CRISPR methodology in Physcomitrella, including the significance of spacing between paired sgRNA targets and the efficacy of NHEJ and HDR in repairing the chromosome when excising a complete locus. As proof-of-principle, we show that the septuple phy− mutant remains gravitropic in light, in line with expectations, and on the basis of data from lower order multiplex knockouts conclude that phy5a is the principal phytochrome responsible for inhibiting gravitropism in light. We expect, therefore, that this mutant collection will be valuable for further studies of phytochrome function and that the methods we describe will allow similar approaches to revealing specific functions in other gene families.

Divergence of the phytochrome gene family predates angiosperm evolution and suggests that Selaginella and Equisetum arose prior to Psilotum

1995 ◽  
Vol 41 (3) ◽  
Author(s):  
H.�. Kolukisaoglu ◽  
S. Marx ◽  
C. Wiegmann ◽  
S. Hanelt ◽  
H.A.W. Schneider-Poetsch
Keyword(s):  
Gene Family ◽  
Angiosperm Evolution ◽  
Phytochrome Gene
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