scholarly journals Editorial Commentary: Is the In Vitro Interferon‐γ Release Assay an Adequate Replacement for the Tuberculin Skin Test?

2002 ◽  
Vol 34 (11) ◽  
pp. 1457-1459 ◽  
Author(s):  
David Nadal
Keyword(s):  
Tuberculin Skin Test ◽  
Skin Test ◽  
Interferon Γ ◽  
Editorial Commentary ◽  
Release Assay

A Summary of the Third Global Interferon-γ Release Assay Symposium

2013 ◽  
Vol 34 (6) ◽  
pp. 619-624 ◽  
Author(s):  
Antonino Catanzaro ◽  
Charles Daley
Keyword(s):  
Immune Response ◽  
Tuberculin Skin Test ◽  
Skin Test ◽  
Interferon Γ ◽  
Enzyme Linked Immunosorbent Assay ◽  
In Vitro Tests ◽  
The Third ◽  
Specific Antigens ◽  
Ifn Γ

Studies over the past several decades have dramatically increased our understanding of the immune response to Mycobacterium tuberculosis infection, and advances in proteomics and genomics have led to a new class of immune-diagnostic tests, termed interferon-γ (IFN-γ) release assays (IGRAs), which appear to obviate many of the problems encountered with the tuberculin skin test (TST). Worldwide, 2 IGRAs are currently commercially available. QuantiFERON-TB Gold In-Tube (Cellestis) is a third-generation product that uses an enzyme-linked immunosorbent assay to measure IFN-γ generated in whole blood stimulated with M. tuberculosis–specific antigens. T-Spot-TB (Oxford Immunotec) employs enzyme-linked immunosorbent spot technology to enumerate the number of purified lymphocytes that respond to M. tuberculosis–specific antigens by producing IFN-γ. These in vitro tests measure the host immune response to M. tuberculosis–specific antigens, which virtually eliminates false-positive cross reactions caused by bacillus Calmette-Guérin vaccination and/or exposure to environmental nontuberculous mycobacteria that plague the interpretation and accuracy of the tuberculin skin test (TST). The high specificity of IGRAs, together with sensitivity commensurate with or better than that of the TST, promises an accurate diagnosis and the ability to focus tuberculosis-control activities on those who are actually infected with M. tuberculosis. The Third Global Symposium was held over a 3-day period and was presented by the University of California, San Diego, Continuing Medical Education department; slides and sound recordings of each presentation are available at http://cme.ucsd.edu/igras/syllabus.html. A moderated discussion is also available at http://cme.ucsd.edu/igrasvideo. This document provides a summary of the key findings of the meeting, specifically focusing on the use of IGRAs in screening healthcare worker populations.

Utility of an Interferon-γ Release Assay for Latent Tuberculosis Diagnosis in a Case of Bullous Pemphigoid

2009 ◽  
Vol 13 (5) ◽  
pp. 280-282 ◽  
Author(s):  
Alfred Goodfellow ◽  
Douglas N. Keeling ◽  
Robert C. Hayes ◽  
Duncan Webster
Keyword(s):  
Tuberculin Skin Test ◽  
Bullous Pemphigoid ◽  
Skin Test ◽  
Latent Tuberculosis Infection ◽  
Latent Tuberculosis ◽  
Interferon Γ ◽  
Tuberculosis Infection ◽  
Mycobacterium Tuberculosis Infection ◽  
Cutaneous Disease ◽  
Release Assay

Background: With increasing use of immunosuppressive therapy, including tumor necrosis factor a inhibitors, there is concern about infectious complications, including reactivation of latent Mycobacterium tuberculosis infection. Routine testing prior to administration of systemic immunosuppression includes the tuberculin skin test, which lacks sensitivity and specificity and may be difficult to interpret in the presence of extensive cutaneous disease. Treatment of individuals with latent tuberculosis infection is recommended when immunosuppressive medications are to be employed. Observations: We report a case in which a diagnosis of latent tuberculosis infection in a patient with extensive bullous pemphigoid was clarified by the use of an interferon-γ release assay after equivocal tuberculin skin test results. Conclusion: Interferon-γ release assays are useful adjuncts to the tuberculin skin test in the diagnosis of latent tuberculosis infection in the setting of extensive cutaneous disease.

scholarly journals Discordance Between Tuberculin Skin Test and Interferon-γ Release Assay in Children Younger Than 5 Years Who Have Been Vaccinated With Bacillus Calmette-Guérin

2015 ◽  
Vol 46 (3) ◽  
pp. 200-206 ◽  
Author(s):  
Ivan Pavić ◽  
Vera Katalinić-Janković ◽  
Jasna Čepin-Bogović ◽  
Arnes Rešić ◽  
Slavica Dodig
Keyword(s):  
Tuberculin Skin Test ◽  
Skin Test ◽  
Interferon Γ ◽  
Bacillus Calmette Guerin ◽  
Release Assay

scholarly journals Monocyte metabolic transcriptional programs associate with resistance to tuberculin skin test/interferon-γ release assay conversion

2021 ◽  
Author(s):  
Jason D. Simmons ◽  
Phu T. Van ◽  
Catherine M. Stein ◽  
Violet Chihota ◽  
Thobani Ntshiqa ◽  
...  
Keyword(s):  
Tuberculin Skin Test ◽  
Skin Test ◽  
Interferon Γ ◽  
Release Assay

Rate of Latent Tuberculosis Infection Detected by Occupational Health Screening of Nurses New to a London Teaching Hospital

2009 ◽  
Vol 30 (6) ◽  
pp. 581-584 ◽  
Author(s):  
Priya Khanna ◽  
Vladyslav Nikolayevskyy ◽  
Fiona Warburton ◽  
Elek Dobson ◽  
Francis Drobniewski
Keyword(s):  
Tuberculin Skin Test ◽  
Skin Test ◽  
Latent Tuberculosis Infection ◽  
Latent Tuberculosis ◽  
Interferon Γ ◽  
Tuberculosis Infection ◽  
Release Assay ◽  
Ifn Γ ◽  
Positive Results ◽  
Tuberculosis Prevalence

The prevalence of latent tuberculosis infection in a cohort of nurses new to a London hospital was 7.6% (13 of 171), using an interferon-γ(IFN-γ) release assay, and 16.2% (24 of 148), using the tuberculin skin test. On multivariate analysis, birth in a country with tuberculosis prevalence of more than 40 cases per 100,000 population was associated with positive results of both the IFN-γ release assay and the tuberculin skin test.

Conversion rates of an interferon-γ release assay and the tuberculin skin test in the serial monitoring of healthcare workers

Infection ◽  
2012 ◽  
Vol 41 (2) ◽  
pp. 511-516 ◽  
Author(s):  
S. Y. Kim ◽  
M. S. Park ◽  
Y. S. Kim ◽  
S. K. Kim ◽  
J. Chang ◽  
...  
Keyword(s):  
Tuberculin Skin Test ◽  
Skin Test ◽  
Healthcare Workers ◽  
Interferon Γ ◽  
Conversion Rates ◽  
Release Assay
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