Effects of Nitrogen Source on Spore Germination and Gametophyte Growth in Psilotum

1990 ◽  
Vol 151 (1) ◽  
pp. 50-53 ◽  
Author(s):  
Dean P. Whittier
Keyword(s):  
Nitrogen Source ◽  
Spore Germination

Spore germination in the Psilotaceae

1994 ◽  
Vol 72 (5) ◽  
pp. 688-692 ◽  
Author(s):  
Dean P. Whittier ◽  
John E. Braggins
Keyword(s):  
Key Words ◽  
Nitrogen Source ◽  
Spore Germination ◽  
Nutrient Medium

Spores of several species of Psilotum and Tmesipteris were sown on a nutrient medium containing minerals and 0.2% glucose. The nutrient medium contained ammonium as the nitrogen source and lacked nitrate. From 52 to 98% of the spores of P. nudum, P. complanatum, T. lanceolata, and T. sigmatifolia germinated after 6 months in the dark. Haploid spores of P. nudum began germinating in less than 2 months; however spores of the two Tmesipteris species reached 50% germination sooner than those of the Psilotum species. Spores of T. tannensis never germinated and those of T. elongata and P. × intermedium rarely germinated (<0.1%) and never formed mature gametophytes. Spores from only one species, T. lanceolata, occasionally germinated in the light, but gametophytic development never proceeded beyond the two-celled stage. Spores of T. lanceolata and T. sigmatifolia stored at −70 °C for 21 months germinated essentially as well as the fresh spores. The nutrient medium appears suitable for studies on gametophytes of the Psilotaceae because it promoted spore germination and gametophytic growth for most of the species tested. Key words: Psilotum, Tmesipteris, spore, germination.

In vitro Evaluation of Ammonium Base Sulfite Liquor as an Energy and Nitrogen Source for Ruminants

1976 ◽  
Vol 42 (4) ◽  
pp. 993-1001 ◽  
Author(s):  
R. P. Kromann ◽  
T. R. Wilson ◽  
G. S. Cantwell
Keyword(s):  
Nitrogen Source ◽  
In Vitro Evaluation

Effects of light, temperature and pH on spore germination and early gametophytic development of Alsophila metteniana

2009 ◽  
Vol 17 (2) ◽  
pp. 182 ◽  
Author(s):  
Du Honghong ◽  
Li Yang ◽  
Li Dong ◽  
Dai Shaojun ◽  
Jiang Chuangdao ◽  
...  
Keyword(s):  
Spore Germination

Does Nitrogen Source Affect the Palatability of Coastal Bermudagrass? 1

1958 ◽  
Vol 50 (3) ◽  
pp. 172-173 ◽  
Author(s):  
Glenn W. Burton ◽  
James E. Jackson ◽  
B. L. Southwell
Keyword(s):  
Nitrogen Source ◽  
Coastal Bermudagrass

Response of No-Till Corn to Nitrogen Source, Rate, and Time of Application

jpa ◽  
1992 ◽  
Vol 5 (4) ◽  
pp. 607-610 ◽  
Author(s):  
K. L. Wells ◽  
W. O. Thom ◽  
H. B. Rice
Keyword(s):  
Nitrogen Source ◽  
Time Of Application ◽  
No Till ◽  
Source Rate

Spring Dead Spot and Burmudagrass Quality As Influenced by Nitrogen Source and Potassium

Crop Science ◽  
1991 ◽  
Vol 31 (6) ◽  
pp. 1674-1680 ◽  
Author(s):  
P. H. Dernoeden ◽  
J. N. Crahay ◽  
D. B. Davis
Keyword(s):  
Nitrogen Source ◽  
Spring Dead Spot

Characteristics and Function of the Chitin Binding Protein from Xenorhabdus nematophila

2019 ◽  
Vol 26 (6) ◽  
pp. 414-422
Author(s):  
Jia Liu ◽  
Ping Song ◽  
Jie Zhang ◽  
Ziyan Nangong ◽  
Xiaobei Liu ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Growth Inhibition ◽  
Insecticidal Activity ◽  
Spore Germination ◽  
Binding Protein ◽  
Colloidal Chitin ◽  
Inhibition Rate ◽  
Xenorhabdus Nematophila ◽  
Chitin Binding Protein ◽  
And Function

Background: Genome sequence analysis (GenBank access No.: FN667742.1) shows that Xenorhabdus nematophila ATCC19061 contains one gene (Xn-cbp) encoding chitin binding protein (Xn-CBP). Objective: The present work aims to clarify the characteristics and function of Xn-CBP from X. nematophila HB310. Methods: In this study, the Xn-cbp gene was cloned and expressed in Escherichia coli BL21 (DE3). Substrate binding assays were performed to explain the ability of Xn-CBP combined with the polysaccharide. The insecticidal toxicity of Xn-CBP against the second-instar larvae of Helicoverpa armigera was determined by feeding method. Besides, the antifungal activity of Xn-CBP against Coniothyrium diplodiella, Verticillium dahlia, and Fusarium oxysporum was tested by spore germination assay and hyphal extension assay. Results: Xn-CBP encoded 199 amino acids with a calculated mass of 28 kDa, which contained a signal peptide and a chitin binding domain. The Bmax and Kd values of Xn-CBP to colloidal chitin were 2.46 and 4.08, respectively. Xn-CBP had insecticidal activity against the H. armigera with a growth inhibition rate of 84.08%. Xn-CBP had the highest spore germination inhibitory effect on C. diplodiella with the inhibition rate of 83.11%. The hyphal growth inhibition rate of Xn-CBP to F. oxysporum, 41.52%, was higher than the other two fungi. Conclusion: The Xn-CBP had the highest binding ability to colloidal chitin and it showed insecticidal activity and antifungal activity. The present study laid a foundation for further exploitation and utilization of X. nematophila.

Hydroxylamines as One-Atom Nitrogen Sources for Metal-Catalyzed Cycloadditions

Synthesis ◽  
2021 ◽  
Author(s):  
Xinjun Luan ◽  
Jingxun Yu
Keyword(s):  
Transition Metal ◽  
Nitrogen Source ◽  
Nucleophilic Reagent ◽  
Nitrogen Sources ◽  
Short Review ◽  
Indole Derivatives ◽  
Electrophilic Amination ◽  
Intramolecular Cycloaddition ◽  
Transition Metal Catalyzed ◽  
Metal Catalyzed

AbstractTransition-metal-catalyzed C–N bond formation is one of the most important pathways to synthesize N-heterocycles. Hydroxylamines can be transformed into a nucleophilic reagent to react with a carbon cation or coordinate with a transition metal; it can also become an electrophilic nitrogen source to react with arenes, alkenes, and alkynes. In this short review, the progress made on transition-metal-catalyzed cycloadditions with hydroxylamines as a nitrogen source is summarized.1 Introduction2 Cycloaddition To Form Aziridine Derivatives2.1 Intramolecular Cycloaddition To Form Aziridine Derivatives2.2 Intermolecular Cycloaddition To Form Aziridine Derivatives3 Cycloaddition To Form Indole Derivatives4 Cycloaddition To Form Other N-Heterocycles4.1 Aza-Heck-Type Amination Reactions4.2 Nitrene Insertion Amination Reactions4.3 Intramolecular Nucleophilic and Electrophilic Amination Reactions5 Conclusion and Outlook

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