Histological Changes in Epidermal and Subepidermal Cell Layers of Begonia rex Induced to Form de Novo Unicellular Hairs, Buds, and Roots

1984 ◽  
Vol 145 (1) ◽  
pp. 55-59 ◽  
Author(s):  
Averil Chlyah
Keyword(s):  
De Novo ◽  
Histological Changes ◽  
Cell Layers

Free and conjugated polyamines during de novo floral and vegetative bud formation in thin cell layers of tobacco

1987 ◽  
Vol 70 (3) ◽  
pp. 453-460 ◽  
Author(s):  
Patrizia Torrigiani ◽  
Maria Maddalena Altamura ◽  
Gabriella Pasqua ◽  
Barbara Monacelli ◽  
Donatella Serafini-Fracassini ◽  
...  
Keyword(s):  
De Novo ◽  
Bud Formation ◽  
Thin Cell Layers ◽  
Conjugated Polyamines ◽  
Cell Layers ◽  
Free And Conjugated Polyamines ◽  
Vegetative Bud

scholarly journals Time Course of the Development of Nonalcoholic Fatty Liver Disease in the Otsuka Long-Evans Tokushima Fatty Rat

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Yi-Sun Song ◽  
Cheng-Hu Fang ◽  
Byung-Im So ◽  
Jun-Young Park ◽  
Yonggu Lee ◽  
...  
Keyword(s):  
Gene Expression ◽  
Liver Disease ◽  
Animal Models ◽  
Fatty Liver ◽  
Fatty Liver Disease ◽  
De Novo ◽  
Histological Changes ◽  
Nonalcoholic Fatty Liver ◽  
Oletf Rats ◽  
Long Evans

Nonalcoholic fatty liver disease (NAFLD) is considered a hepatic manifestation of metabolic syndrome. In this study, we investigated histological and biochemical changes in NAFLD and the gene expression involvingde novolipogenesis in Otsuka Long-Evans Tokushima fatty (OLETF) rats. We used OLETF rats and Long-Evans Tokushima Otsuka (LETO) rats as animal models of NAFLD and as controls, respectively. Consistent observations were made at 4-week intervals up to 50 weeks of age, and all rats were fed ad libitum with standard food. Biochemical and histological changes were observed, and gene expression involved inde novolipogenesis was measured using real-time polymerase chain reactions. As a results hepatic micro- and macrovesicular steatosis and hepatocyte ballooning were evident in the OLETF rats at 22–38 weeks of age but disappeared after 42 weeks; no fibrosis or collagen deposition was observed. Gene expression involved inde novolipogenesis followed a pattern similar to that of the histological changes. In conclusion, in the absence of dietary manipulation, hepatic steatosis in OLETF rats is evident at 22–38 weeks and declines after 42 weeks. Therefore, OLETF rats at 22–38 weeks are recommended as animal models of hepatic steatosis.

Control of morphogenetic pathways in thin cell layers of tobacco by pH

1989 ◽  
Vol 67 (3) ◽  
pp. 650-654 ◽  
Author(s):  
A. Cousson ◽  
P. Toubart ◽  
K. Tran Thanh Van
Keyword(s):  
Butyric Acid ◽  
De Novo ◽  
Culture Media ◽  
Callus Formation ◽  
Medium Ph ◽  
Vegetative Buds ◽  
Cell Layers ◽  
Initial Medium ◽  
Vegetative Bud

Thin cell layer explants of tobacco were floated in vitro on the surface of liquid culture media. The initial exogenous concentrations of indolyl-3-butyric acid, and kinetin, the initial medium pH, and the explant density were varied. Various patterns of de novo and direct differentiation without any intermediate callus (flower, vegetative bud, root) as well as the absence of morphogenesis and callus formation without any subsequent organogenesis were separately controlled on 100% of the explants. On the same exogenous combination of glucose, indolyl-3-butyric acid, and kinetin, changes in initial medium pH changed the pattern of morphogenesis. For a given initial exogenous indolyl-3-butyric acid concentration, vegetative buds were obtained at either pH 6.1 or 7.8, whereas a mixture of flowers and vegetative buds was obtained at pH 6.8. Furthermore, changes in explant density changed the morphogenetic response. It is suggested that the effects of the initial medium pH and explant density on morphogenesis may be related partially to modifications of the physicochemical properties of the cell wall and (or) plasmalemma.

scholarly journals Immunocytochemical studies of cardiac myofibrillogenesis in early chick embryos. III. Generation of fasciae adherentes and costameres.

1989 ◽  
Vol 108 (1) ◽  
pp. 43-53 ◽  
Author(s):  
K T Tokuyasu
Keyword(s):  
Cell Membranes ◽  
Spatial Information ◽  
De Novo ◽  
Three Dimensional ◽  
Myocardial Cell ◽  
Double Labeling ◽  
Rhodamine Phalloidin ◽  
Somite Stage ◽  
Myocardial Wall ◽  
Cell Layers

To study whether the first myofibrils are separate from or firmly bound to the myocytic cell membranes, whole mount preparations of 6-12-somite-stage chick embryonic hearts were examined by fluorescence microscopy after double labeling with antibodies to vinculin (fluorescein-conjugated) and rhodamine-phalloidin, or with antibodies to titin (rhodamine-conjugated) and nitrobenz-oxadiazole-phallacidin. When a small number of myofibrils appeared for the first time at the nine somite stage, most of them were already bound to the cell membranes through zonulae adherentes, fasciae adherentes, or costameres. In the outer of the two myocardial cell layers, in which the myocytes were closely in contact with each other along polygonal boundaries, fasciae adherentes and costameres developed at the boundaries, apparently by conversion of preexisting zonulae adherentes. On the other hand, in the inner cell layer, in which myocytes were more loosely associated with each other, both costameres and fasciae adherentes appeared to develop de novo, the former in association with the inner surface of the myocardial wall and the latter at the intercellular boundaries. The myofibrillar tracks in the inner layer followed long and smooth courses and were as a whole aligned in the circumferential direction of the tubular heart wall from the earliest stage of myofibril formation. Those in the outer layer were arranged in a pattern of two- or three-dimensional networks in the 9-10 somite stage, although many myofibrils were also circumferentially directed. The fact that the majority of the first myofibrils were already bound to the cell membranes in a directed manner suggests that myocytes at the earliest stage of myofibril formation are endowed with spatial information that directs the organization of nascent myofibrils. It is proposed that the myocyte cell membranes perform an essential role in cardiac myofibrillogenesis.

Influence of ionic composition of the culture medium on de novo flower formation in tobacco thin cell layers

1993 ◽  
Vol 71 (3) ◽  
pp. 506-511 ◽  
Author(s):  
Alain Cousson ◽  
Kiem Tran Thanh Van
Keyword(s):  
Nicotiana Tabacum ◽  
Nitrogen Source ◽  
De Novo ◽  
Ionic Composition ◽  
Flower Formation ◽  
Sole Nitrogen Source ◽  
Thin Cell Layers ◽  
Initial Control ◽  
Cell Layers ◽  
External Ph

Floral and vegetative organogenesis in thin cell layers of Nicotiana tabacum cv. Samsun was studied in relation to the ionic composition of the medium. The control liquid medium was buffered to pH 5.5. NH4+, NO3−, K+, and Ca2+ were separately varied between 0 or 0.1 times and 2, 3 or 4 times their initial control concentration. Increasing the concentrations of NO3−, and Ca2+ above their control concentration promoted the formation of vegetative buds instead of flowers. When K+ at 80.0 mM, Na+ at 78.9 mM, and NH4+ as the sole nitrogen source used, neither flower formation nor morphogenesis were observed in 70, 100, and 100% of the explants, respectively. NH4+ as the sole nitrogen source acidified the medium to pH 4.2, whereas the other ionic conditions did not change the external pH. NO3− at 7.9 mM prevented this acidification and allowed flower formation in 75% of the explants. This acidification could increase the K+ and Na+ influxes via a possible lowering of the intracellular pH. This hypothesis corroborates the morphogenic effect of K+ and Na+. Key words: absence of morphogenesis, acidification of medium, floral and vegetative organogenesis, ionic composition of the medium, thin cell layers, Nicotiana tabacum cv. Samsun.

scholarly journals Abnormal fibrillin assembly by dermal fibroblasts from two patients with Marfan syndrome

1994 ◽  
Vol 124 (6) ◽  
pp. 997-1004 ◽  
Author(s):  
CM Kielty ◽  
CA Shuttleworth
Keyword(s):  
Marfan Syndrome ◽  
Time Course ◽  
De Novo ◽  
Dermal Fibroblast ◽  
Clinical Manifestations ◽  
Connective Tissue Disorder ◽  
Dermal Fibroblasts ◽  
Relative Molecular Mass ◽  
Cell Layers ◽  
The Relationship

The microfibrillar glycoprotein fibrillin is linked to the Marfan syndrome, an autosomal dominant connective tissue disorder. In this study, fibrillin synthesis, deposition and assembly has been investigated in Marfan dermal fibroblast lines from two unrelated patients for whom distinct mutations in the fibrillin gene FBN1 have been identified. In patient NB, a point mutation has occurred which causes an amino acid substitution and the other patient (GK) has a deletion in one allele. The two cell lines were broadly comparable with respect to de novo fibrillin synthesis and its distribution between medium and cell layer compartments. Electrophoresis of fibrillin immunoprecipitates confirmed the presence of fibrillin in medium and cell layers. GK cells secreted an additional higher relative molecular mass fibrillin-immunoreactive component. The time-course of fibrillin secretion was similar for the two lines, but differences in fibrillin aggregation were apparent. Rotary shadowing electron microscopy of extracted cell layers demonstrated the presence of abundant and extensive microfibrils in NB cell layers. These were abnormal in their gross morphology in comparison to microfibrils isolated from control cultures. No periodic microfibrillar structures were isolated from GK cell layers. These studies underline the need to classify fibrillin defects in terms of biochemical and ultrastructural criteria. Examination of the effects of individual mutations on microfibril organization will be particularly informative in elucidating the relationship between microfibril dysfunction and the complex clinical manifestations of Marfan patients.

scholarly journals De Novo Induction of Atherosclerosis byChlamydia pneumoniae in a Rabbit Model

1999 ◽  
Vol 67 (11) ◽  
pp. 6048-6055 ◽  
Author(s):  
Ignatius W. Fong ◽  
Brian Chiu ◽  
Esther Viira ◽  
Dan Jang ◽  
James B. Mahony
Keyword(s):  
Chlamydia Pneumoniae ◽  
De Novo ◽  
Rabbit Model ◽  
Tissue Growth ◽  
Respiratory Pathogen ◽  
Cholesterol Diet ◽  
Histological Changes ◽  
Tract Pathogen ◽  
Induced Changes ◽  
Grade Iii

ABSTRACT Chlamydia pneumoniae, a bacterial respiratory tract pathogen, has been associated with atherosclerosis in humans. C. pneumoniae infection of the respiratory tracts of rabbits fed a noncholesterol diet induced changes of atherosclerosis of the aorta in 6 (26.1%) of 23 animals after a single inoculum at 3 months. Multiple inocula given three times within 6 weeks resulted in grade III atherosclerosis in 8 (34.8%) of 23 rabbits, with an additional 5 (21.7%) showing increased myxoid changes in the intima-media junction and exhibiting 8 (34.8%) focal periaortitis. Control animals inoculated with carrier broth (n = 24), HEp-2 cells (n = 12), or another respiratory pathogen,Mycoplasma pneumoniae (n = 32), produced no changes of atherosclerosis after 3 months. The histological changes were dissimilar (fewer foam cells) from those of rabbits fed a 0.5% cholesterol diet but were highly similar to or indistinguishable from changes in rabbits fed a 0.15% cholesterol diet (similar to that of humans). Proinflammatory cytokines and tissue growth factors were more consistently detected in cholesterol-induced aortic lesions than those induced by C. pneumoniae. These data are compatible with de novo induction of atherogenesis by C. pneumoniae in rabbits and suggest that C. pneumoniae may be important in the pathogenesis of atherosclerosis in humans.

scholarly journals Lung transplant after 6 months on ECMO support for SARS-CoV-2-induced ARDS complicated by severe antibody-mediated rejection

2021 ◽  
Vol 8 (1) ◽  
pp. e001036
Author(s):  
Sandra Lindstedt ◽  
Edgar Grins ◽  
Hillevi Larsson ◽  
Johan Nilsson ◽  
Hamid Akbarshahi ◽  
...  
Keyword(s):  
De Novo ◽  
Acute Hypoxia ◽  
High Dose ◽  
Close Monitoring ◽  
Post Transplantation ◽  
Current Case ◽  
Histological Changes ◽  
Antibody Mediated Rejection ◽  
End Stage

There have been a few reports of successful lung transplantation (LTx) in patients with SARS-CoV-2-induced acute respiratory distress syndrome (ARDS); however, all reports were with rather short follow-up. Here we present a 62-year-old man without prior lung diseases. Following SARS-CoV-2-induced ARDS and 6 months of extracorporeal membrane oxygenation, he underwent LTx. 3 months post-transplantation he developed acute hypoxia requiring emergency intubation. Chest imaging showed acute rejection, and de novo DQ8-DSA was discovered. He was treated with a high dose of corticosteroids and plasmapheresis and was extubated 4 days later, yet the de novo DQ8-DSA remained. After sessions of plasmapheresis and rituximab, the levels of de novo DQ8-DSA remained unchanged. Nine months post-transplantation the patient died of respiratory failure. We herein discuss the decision to transplant, the transplantation itself and the postoperative course with severe antibody-mediated rejection. In addition, we evaluated the histological changes of the explanted lungs and compared these with end-stage idiopathic pulmonary fibrosis tissue, where both similarities and differences are seen. With the current case experience, one might consider close monitoring regarding DSA, and gives further support that LTx should only be considered for very carefully selected patients.

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