Pectolytic Enzyme Activity in Various Parts of the Cucumber Plant and Fruit

1951 ◽  
Vol 113 (2) ◽  
pp. 216-221 ◽  
Author(s):  
Thomas A. Bell
1972 ◽  
Vol 18 (7) ◽  
pp. 1065-1072 ◽  
Author(s):  
Hassan A. Melouk ◽  
Chester E. Horner

Pectolytic enzyme activity of P. strasseri was highest in filtrates from cultures 5 and 23 days old, as determined by reducing-groups assay (RGA). Pectolytic enzyme activity, as determined by viscosity-reduction assay (VRA), was highest in cultures 7 days old. Macerating enzyme activities were highest in cultures 10 days old. Young peppermint-rhizome sections were more susceptible to maceration by P. strasseri filtrates than older sections. Colored phenolic compounds were released from macerated rhizomes and amounts released were correlated with degree of maceration.Differential inhibition of pectolytic enzyme activity (as judged by VRA and RGA assays) by a range of phenolic compounds suggests that at least two pectolytic enzymes capable of hydrolyzing α-1,4 linkages of pectic substances are produced by P. strasseri.Highest activities of pectolytic and macerating enzyme in extracts from diseased rhizomes were detected 5 days after inoculation with P. strasseri. Extracts from healthy rhizomes did not show pectolytic and macerating enzyme activity.Healthy peppermint-rhizome tissues had higher activities of polyphenoloxidase than diseased tissues; also, polyphenoloxidase activities in inoculated rhizomes decreased as time of incubation increased.


1963 ◽  
Vol 48 (4) ◽  
pp. 439-444 ◽  
Author(s):  
M. S. Nour El Dein ◽  
M. S. Sharkas

1996 ◽  
Vol 61 (2) ◽  
pp. 311-314 ◽  
Author(s):  
F. ARTÉS ◽  
A. CANO ◽  
J.P. FERNÁNDEZ-TRUJILLO

1969 ◽  
Vol 47 (6) ◽  
pp. 1007-1010 ◽  
Author(s):  
L. Van Den Berg ◽  
S. M. Yang

B. cinerea and S. sclerotiorum growing in a nutrient medium on the surface of carrots at 20 °C produced significantly more extracellular pectolytic enzymes when the carrots were exposed to 94–96% relative humidity than when exposed to 98–100% relative humidity. Tests in which these organisms were grown in a nutrient solution containing pectic substances showed that they produced pectolytic enzymes in significant quantities only when readily metabolizable sources of energy (e.g. ethanol, carbohydrates) were not available. These results suggest that the low relative humidity increased enzyme production by concentrating nutrients on the carrot surface to the point where they inhibited growth of the organisms and stimulated enzyme production. Tests also showed that pectolytic enzyme activity on the surface of unwashed carrots stored 9 months at 0–1 °C was substantially higher at 90–95% relative humidity than at 98–100% relative humidity. The results indicated that reduced decay at 98–100% relative humidity was largely due to lower pectolytic enzyme production.


1978 ◽  
Vol 17 (4) ◽  
pp. 797-799 ◽  
Author(s):  
M. Kroh ◽  
Mary W. Loewus

1980 ◽  
Vol 19 (12) ◽  
pp. 2553-2555 ◽  
Author(s):  
Ruth Ben-Arie ◽  
Lillian Sonego

2021 ◽  
Vol 21 (2) ◽  
Author(s):  
Pawar A. B.

Alternaria alternata is a potential pathogen of Tectona grandis L.f., was isolated from diseased Tectona grandis L.f. leaves from Nashik and used for the present study. Pathogen was grown on the Czapek-Dox liquid medium substituting or adding different carbon, nitrogen to study cellulolytic and pectolytic enzyme production and total phenol production. The activity of enzyme was observed on the 8th day of incubation period. A great extent of growth variation was observed on different carbon, nitrogen. Among the carbon source) the maximum loss in percentage viscosity maximum in lactose and fructose. While minimum in glucose and dextrose as compared to other nitrogen source. From nitrogen source the cellulolytic enzyme activity was maximum in control and cobalt nitrate followed by similar activity in potassium nitrate and nickel nitrate. While minimum cellulolytic enzyme activity was seen in barium nitrate.. Variation was also observe in pectolytic enzyme activity. the cellulolytic enzyme activity was maximum in glucose while minimum in dextrose as compared to lactose, control and fructose.. From nitrogen source the cellulose activity was maximum in potasium nitrate and minimum in cobalt nitrate as compared to nickel nitrate, barium nitrate and control.


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