scholarly journals The Promise and Pitfalls of Telomere Region–Specific Probes

2000 ◽  
Vol 67 (5) ◽  
pp. 1356-1359 ◽  
Author(s):  
Blake C. Ballif ◽  
Catherine D. Kashork ◽  
Lisa G. Shaffer
Keyword(s):  
Telomere Region

scholarly journals Terminal Retrotransposons Activate a Subtelomeric white Transgene at the 2L Telomere in Drosophila

Genetics ◽  
2001 ◽  
Vol 158 (3) ◽  
pp. 1111-1123 ◽  
Author(s):  
Mikhail D Golubovsky ◽  
Alexander Y Konev ◽  
Marika F Walter ◽  
Harald Biessmann ◽  
James M Mason
Keyword(s):  
Reporter Gene ◽  
Chromatin Structure ◽  
Chromosome 2 ◽  
Putative Promoter ◽  
Enhancer Element ◽  
Altered Expression ◽  
Transposition Activity ◽  
Eye Color ◽  
P Elements ◽  
Telomere Region

Abstract Genetically marked P elements inserted into the subtelomeric satellites of Drosophila show repression and variegation of the reporter gene. One such white+ reporter, inserted between the subtelomeric satellite and the terminal HeT-A array in the left arm of chromosome 2 (2L), is sensitive to its context; changes in the structure of the telomere region can be identified by changes in eye color. Addition of HeT-A or TART elements to the 2L terminus increases w+ expression, and loss of sequence from the end decreases expression. This indicates that the telomeric retrotransposons in Drosophila have an activating influence on the repressed subterminal reporter gene. Changes in eye color due to altered expression of the transgene also allow the detection of interactions between homologous telomeres. The 2L arms that terminate in long HeT-A/TART arrays showed increased expression of the subterminal w+ transgene when the terminal repeats on the homologue are absent or markedly shorter. We propose that the chromatin structure of the terminal HeT-A/TART array and the activity of a putative promoter/enhancer element on HeT-A are affected by telomeric interactions. Such trans-activation may reflect control over HeT-A transcription and, thus, transposition activity.

DNA organization and polymorphism of a wild-type Drosophila telomere region

Chromosoma ◽  
1995 ◽  
Vol 104 (4) ◽  
pp. 229-241 ◽  
Author(s):  
M. F. Walter ◽  
C. Jang ◽  
B. Kasravi ◽  
J. Donath ◽  
B. M. Mechler ◽  
...  
Keyword(s):  
Wild Type ◽  
Dna Organization ◽  
Telomere Region

scholarly journals Expression of human growth hormone in the milk of transgenic rabbits with transgene mapped to the telomere region of chromosome 7q

2012 ◽  
Vol 53 (4) ◽  
pp. 435-442 ◽  
Author(s):  
Daniel Lipinski ◽  
Joanna Zeyland ◽  
Marlena Szalata ◽  
Andrzej Plawski ◽  
Malgorzata Jarmuz ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Transgenic Rabbits ◽  
Telomere Region ◽  
Chromosome 7Q

DNA organization and polymorphism of a wild-type Drosophila telomere region

Chromosoma ◽  
1995 ◽  
Vol 104 (4) ◽  
pp. 229-241 ◽  
Author(s):  
M. F. Walter ◽  
C. Jang ◽  
B. Kasravi ◽  
J. Donath ◽  
B. M. Mechler ◽  
...  
Keyword(s):  
Wild Type ◽  
Dna Organization ◽  
Telomere Region

Adaptation of a preembedding electron microscopic in situ hybridization for detection of the telomere region in human interphase nuclei

1998 ◽  
Vol 31 (1) ◽  
pp. 49-52
Author(s):  
Kouko Nagano-Tatsumi ◽  
Satomi Haga ◽  
Manabu Maeda ◽  
Yoshiaki Nawa ◽  
Hiroshi Yamamoto
Keyword(s):  
In Situ Hybridization ◽  
Electron Microscopic ◽  
Interphase Nuclei ◽  
Telomere Region

scholarly journals A complete set of marked telomeres in Saccharomyces cerevisiae for physical mapping and cloning.

Genetics ◽  
1995 ◽  
Vol 139 (1) ◽  
pp. 125-136
Author(s):  
E J Louis ◽  
R H Borts
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Restriction Enzymes ◽  
Chromosomal Dna ◽  
Lithium Acetate ◽  
Single Strain ◽  
Strand Breaks ◽  
Physical Maps ◽  
Telomere Region ◽  
Complete Set ◽  
Integrating Vector

Abstract Each telomere in a single strain (S288C) of Saccharomyces cerevisiae was marked with a URA3 containing integrating vector having telomeric TG1-3 sequences. Efficiency of integrative transformation was enhanced by creating single random double-strand breaks in the integrating vector using DNAseI in the presence of Mn2+ ions. A total of 327 transformants were screened by CHEF gels of intact chromosomal DNA. Transformants with homology to the vector at particular chromosomal bands were then screened by Southern analysis with several restriction enzymes to confirm telomeric locations. CHEF gels of NotI and/or SfiI digests were also analyzed to determine left or right arm locations. In some cases allelism of marked telomeres was determined genetically. Transformation was performed by lithium acetate and electroporation with varying results. Electroporation resulted in 50% (75/150) of the integrants at the internal URA3 location rather than telomeres. There were also two rearrangements involving URA3 and the telomere of another chromosome. Lithium acetate transformation resulted in fewer integrants at the internal URA3 location (5/84) and no rearrangements. All telomeres were marked with approximately the same efficiency ranging from 0 to 11 hits in the first 240 transformants. These marked telomeres can be used to complete the physical maps of chromosomes in which the telomere regions are absent. The marked telomeres can be cloned with the appropriate restriction enzymes, thus completing the cloning of individual chromosomes for sequencing projects. The analysis of these clones will lead to a better understanding of telomere region biology. The methodology can also be applied to telomeres of other organisms once they are cloned as telomeric YACs.

scholarly journals Dissecting the telomere region of barley chromosome 5HL using rice genomic sequences as references: new markers for tracking a complex region in breeding

2010 ◽  
Vol 27 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Xiao-Qi Zhang ◽  
Chengdao Li ◽  
Joe Panozzo ◽  
Sharon Westcott ◽  
Guoping Zhang ◽  
...  
Keyword(s):  
Barley Chromosome ◽  
Genomic Sequences ◽  
Complex Region ◽  
Telomere Region

scholarly journals The Promise and Pitfalls of Telomere Region–Specific Probes

2000 ◽  
Vol 67 (5) ◽  
pp. 1356-1358 ◽  
Author(s):  
Blake C. Ballif ◽  
Catherine D. Kashork ◽  
Lisa G. Shaffer
Keyword(s):  
Telomere Region
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