Intestinal Nutrient Uptake Measurements and Tissue Damage: Validating the Everted Sleeves Method

2000 ◽  
Vol 73 (4) ◽  
pp. 454-460 ◽  
Author(s):  
J. Matthias Starck ◽  
William H. Karasov ◽  
Daniel Afik
Author(s):  
Hilton H. Mollenhauer

Various means have been devised to preserve biological specimens for electron microscopy, the most common being chemical fixation followed by dehydration and resin impregnation. It is intuitive, and has been amply demonstrated, that these manipulations lead to aberrations of many tissue elements. This report deals with three parts of this problem: specimen dehydration, epoxy embedding resins, and electron beam-specimen interactions. However, because of limited space, only a few points can be summarized.Dehydration: Tissue damage, or at least some molecular transitions within the tissue, must occur during passage of a cell or tissue to a nonaqueous state. Most obvious, perhaps, is a loss of lipid, both that which is in the form of storage vesicles and that associated with tissue elements, particularly membranes. Loss of water during dehydration may also lead to tissue shrinkage of 5-70% (volume change) depending on the tissue and dehydrating agent.


Author(s):  
R. W. Cole ◽  
J. C. Kim

In recent years, non-human primates have become indispensable as experimental animals in many fields of biomedical research. Pharmaceutical and related industries alone use about 2000,000 primates a year. Respiratory mite infestations in lungs of old world monkeys are of particular concern because the resulting tissue damage can directly effect experimental results, especially in those studies involving the cardiopulmonary system. There has been increasing documentation of primate parasitology in the past twenty years.


2010 ◽  
Vol 80 (2) ◽  
pp. 131-143 ◽  
Author(s):  
Pedro Gonçalves ◽  
João R. Araújo ◽  
Fátima Martel

We studied the effect of some mineral waters and some of their constituents on the apical uptake of 14C-butyrate (14C-BT) and 3H-O-methyl-D-glucose (3H-OMG) by Caco-2 cells. Uptake of 14C-BT increased after a 20-minute exposure to 1 % (v/v) distilled water, and, compared to distilled water, it was decreased by Pedras Salgadas® 1 % (v/v) and Melgaço® 5 % (v/v), and increased by Vidago® 5 % (v/v). Moreover, it increased after a 48-hour exposure to Vidago® or Melgaço® waters (5 % (v/v)). Also, uptake of 14C-BT was reduced after a 20-minute exposure to MgCl2, MgSO4, or CaCl2. Uptake of 3H-OMG was reduced after a 20-minute exposure to Melgaço® water [1 % (v/v)], when compared to distilled water. Also, a 48-hour exposure to Pedras Salgadas® or Melgaço® water (5 % (v/v)) increased and decreased uptake, respectively. Finally, uptake of 3H-OMG decreased after a 20-minute exposure to MgSO4 or NaF. In conclusion, uptake of 14C-BT and 3H-OMG by Caco-2 cells is differently modulated by distinct mineral waters.


2010 ◽  
Vol 48 (01) ◽  
Author(s):  
R Dayoub ◽  
C Dorn ◽  
C Hackl ◽  
O Stölzing ◽  
WE Thasler ◽  
...  

2007 ◽  
Vol 35 (2) ◽  
pp. 253-256
Author(s):  
Milan Biberdžić ◽  
Ivica Đalović ◽  
Aleksandar Paunović ◽  
Ilija Komljenović

1987 ◽  
Vol 49 (1) ◽  
pp. 24-27 ◽  
Author(s):  
Keiko TANAKA ◽  
Yuji SHUDO ◽  
Akira TANAKA

2018 ◽  
Vol 102 (1) ◽  
pp. 11-14 ◽  
Author(s):  
Gabriel Barth ◽  
Eros Francisco ◽  
Juliana Tamie Suyama
Keyword(s):  

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