The Physiological Basis for Altered Na+ and Cl− Movements across the Gills of Rainbow Trout (Oncorhynchus mykiss) in Alkaline (pH = 9.5) Water

1999 ◽  
Vol 72 (3) ◽  
pp. 360-368 ◽  
Author(s):  
Michael P. Wilkie ◽  
Pierre Laurent ◽  
Chris M. Wood
1995 ◽  
Vol 52 (12) ◽  
pp. 2583-2593 ◽  
Author(s):  
Deke T. Gundersen ◽  
Lawrence R. Curtis

Rainbow trout (Oncorhynchus mykiss) were acclimated for 10 d to soft (0.1 mM as CaCO3) or hard (1.0 mM as CaCO3) water at weakly alkaline pH (8.06–8.34). Following acclimation, individual gill arches were removed for examining the effects of low hardness or high hardness acclimation on gill water permeability, gill Ca2+ interactions, and gill aluminum interactions. Isolated gill arches were exposed to water of varying Ca2+ (0.0–1.0 mM) and aluminum (3.7–37 μM) concentration for osmotic permeability experiments. High hardness acclimated gills had significantly greater percent weight gain over time caused by osmotic water entry than low hardness acclimated gills, when exposed to distilled water (32.34 ± 1.15 and 24.86 ± 0.62%, respectively, after 60-min incubations); these differences were absent when Ca2+ (0.1–1.0 mM) was added to the incubation medium. Gill arch Ca2+ binding experiments resolved two gill surface binding site populations, which differed in their Ca2+ binding affinity. The higher affinity sites were probably associated with gill membrane permeability, because low hardness acclimated gills had more such sites (binding capacity, 0.322 ± 0.027 μmol Ca2+∙g−1) and less permeable gills than high hardness acclimated gills (binding capacity, 0.198 ± 0.004 μmol Ca2+∙g−1). Aluminum had little influence on gill permeability and gill Ca2+ binding.


1994 ◽  
Vol 51 (6) ◽  
pp. 1345-1355 ◽  
Author(s):  
Deke T. Gundersen ◽  
Sjahrul Bustaman ◽  
Wayne K. Seim ◽  
Lawrence R. Curtis

Juvenile rainbow trout (Oncorhynchus mykiss) were exposed for either 96 h (acute) or 16 d (subacute) to various combinations of aluminum and hardness concentrations or aluminum and humic acid concentrations between pH 7.14 and 8.58. Aluminum-induced mortality was higher at weakly alkaline pH (7.95–8.58) (attributed to up to 10-fold higher filterable aluminum concentrations) than near-neutral pH (7.14–7.64). Growth rates were higher in 16-d hardness tests at weakly alkaline pH (0.188–1.600% of initial weight∙d−1) than for fish exposed to similar aluminum concentrations at near-neutral pH (−0.535–0.756%∙d−1). This suggested that polymeric and colloidal forms of aluminum were more potent than soluble forms in restricting growth. Hardness and humic acid appeared most protective to trout against subacute aluminum toxicity. Cumulative mortality of trout exposed to 1.50 mg aluminum∙L−1 at 103 or 20 mg∙L−1 hardness as CaCO3 was 10 and 45%, respectively. Hardness did not significantly protect against aluminum-induced growth inhibition. Trout exposed for 16 d to aluminum (0.53–2.56 mg∙L−1) and humic acid 4.31–5.23 mg∙L−1) had higher specific growth rates and decreased mortality than those exposed to aluminum and ho humic acid at any pH.


Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
A Ghasemi Pirbalouti ◽  
E Pirali ◽  
G Pishkar ◽  
S Mohammadali Jalali ◽  
M Reyesi ◽  
...  

2014 ◽  
Author(s):  
Silvia Gonzalez-Rojo ◽  
Cristina Fernandez-Diez ◽  
Marta Lombo ◽  
Vanesa Robles Rodriguez ◽  
Herraez Maria Paz

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