scholarly journals Immune Complexes from Serum of Patients with Lyme Disease ContainBorrelia burgdorferiAntigen and Antigen‐Specific Antibodies: Potential Use for Improved Testing

2000 ◽  
Vol 182 (2) ◽  
pp. 534-539 ◽  
Author(s):  
Michael Brunner ◽  
Leonard H. Sigal
Keyword(s):  
Lyme Disease ◽  
Immune Complexes ◽  
Specific Antibodies ◽  
Potential Use

Immune complexes in early Lyme disease

2007 ◽  
Vol 53 (12) ◽  
pp. 1375-1377 ◽  
Author(s):  
Daniela Lenčáková ◽  
Astéria Štefančíková ◽  
Renáta Ivanová ◽  
Branislav Peťko
Keyword(s):  
Polyethylene Glycol ◽  
Lyme Disease ◽  
Immune Complexes ◽  
Erythema Migrans ◽  
Serological Diagnosis ◽  
Early Disease ◽  
Specific Antibodies ◽  
Igm Antibodies

The study investigated the presence of Borrelia -specific antibodies captured in immune complexes (ICs) in patients with early Lyme disease manifested by erythema migrans. Out of 18 patients, 15 (83.3%) tested positive for polyethylene glycol-precipitated ICs containing IgM antibodies, while only 4 (22.2%) were IgG positive. These results are in accordance with our findings obtained by standard ELISA and recombinant blot, which indicated that ICs might be used for serological diagnosis of the early disease.

Sequestration of antibody to Borrelia burgdorferi in immune complexes in seronegative Lyme disease

The Lancet ◽  
1990 ◽  
Vol 335 (8685) ◽  
pp. 312-315 ◽  
Author(s):  
S.E. Schutzer ◽  
P.K. Coyle ◽  
A.L. Belman ◽  
M.G. Golightly ◽  
J. Drulle
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Immune Complexes

scholarly journals STUDIES ON ANTIGEN-ANTIBODY COMPLEXES

1971 ◽  
Vol 133 (4) ◽  
pp. 713-739 ◽  
Author(s):  
Mart Mannik ◽  
William P. Arend ◽  
Anthony P. Hall ◽  
Bruce C. Gilliland
Keyword(s):  
Immune Complexes ◽  
Disulfide Bonds ◽  
Complement Fixation ◽  
Solid Phase ◽  
Complement Components ◽  
Specific Antibodies ◽  
Optimal Function ◽  
Soluble Immune Complexes ◽  
Rapid Removal ◽  
Antigen Antibody

Solid phase immunoadsorbents were prepared by coupling antigens to agarose. With this technique specific antibodies were easily isolated in large amounts. The γG-globulin class of antibodies isolated in this manner were not denatured as judged by their normal biological half-life in rabbits. Soluble immune complexes at fivefold antigen excess were prepared from isolated specific antibodies and HSA, human λ-chains, human λG-globulins, and a Waldenström's macroglobulin as antigens. In all these preparations a characteristic immune complex was encountered that represented the smallest stable antigen-antibody union. In the HSA-anti-HSA system they were found to be AgAb2 complexes, and Ag2Ab complexes in the γG-anti-γG system. These stable complexes fixed complement ineffectively. Also, a spectrum of larger complexes was present in each system, and these complexes fixed complement effectively. With intact antibodies the disappearance curves of immune complexes from the circulation were composed of three exponential components. The immune complexes larger than AgAb2 were quickly removed from the circulation with half-lives of 0.09–0.37 hr. Their clearance was not dependent on complement components, in that depletion of complement by cobra venom factor and aggregated γG-globulin did not alter the pattern of their removal from the circulation. However, when the interchain disulfide bonds of antibodies were reduced and alkylated, the removal of the λ-anti-λ, HSA-anti-HSA, and γG-anti-γG complexes was altered. In these experiments the disappearance curves were composed of two exponential components and the rapid removal of the greater than AgAb2 complexes did not occur. The immune complexes prepared from reduced and alkylated antibodies fixed complement ineffectively. The presented data indicate that the rapid removal of circulating immune complexes, containing γG-globulin molecules as antibodies, depends primarily on the number of antibodies involved. Furthermore, complement fixation is not involved in the rapid removal of such complexes. Nevertheless, the rapid removal of immune complexes and their ability to fix complement have similarities for optimal function in that both processes require intact interchain disulfide bonds of antibodies and complexes that exceed the AgAb2 combination.

scholarly journals The Tick Salivary Protein Salp15 Inhibits the Killing of Serum-Sensitive Borrelia burgdorferi Sensu Lato Isolates

2008 ◽  
Vol 76 (7) ◽  
pp. 2888-2894 ◽  
Author(s):  
Tim J. Schuijt ◽  
Joppe W. R. Hovius ◽  
Nathalie D. van Burgel ◽  
Nandhini Ramamoorthi ◽  
Erol Fikrig ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Ixodes Scapularis ◽  
Membrane Attack Complex ◽  
Salivary Protein ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Protective Effects ◽  
Tick Saliva ◽  
Specific Antibodies ◽  
Normal Human

ABSTRACT Borrelia burgdorferi, the agent of Lyme disease, is transmitted by ticks. During transmission from the tick to the host, spirochetes are delivered with tick saliva, which contains the salivary protein Salp15. Salp15 has been shown to protect spirochetes against B. burgdorferi-specific antibodies. We now show that Salp15 from both Ixodes ricinus and Ixodes scapularis protects serum-sensitive isolates of Borrelia burgdorferi sensu lato against complement-mediated killing. I. ricinus Salp15 showed strong protective effects compared to those of I. scapularis Salp15. Deposition of terminal C5b to C9 (one molecule each of C5b, C6, C7, and C8 and one or more molecules of C9) complement complexes, part of the membrane attack complex, on the surface of B. burgdorferi was inhibited in the presence of Salp15. In the presence of normal human serum, serum-sensitive Borrelia burgdorferi requires protection against complement-mediated killing, which is provided, at least in part, by the binding to the tick salivary protein Salp15.

Comparative study of assays detecting circulating immune complexes and specific antibodies in patients infected with Toxocara canis

1987 ◽  
Vol 61 (3) ◽  
pp. 196-202 ◽  
Author(s):  
C. Aguila ◽  
C. Cuéllar ◽  
S. Fenoy ◽  
J. L. Guillén
Keyword(s):  
Monoclonal Antibodies ◽  
Comparative Study ◽  
Immune Complexes ◽  
Sandwich Elisa ◽  
Toxocara Canis ◽  
Circulating Immune Complexes ◽  
Soluble Antigen ◽  
Elisa Method ◽  
Active Infection ◽  
Specific Antibodies

ABSTRACTA sandwich ELISA method using previously described E/S antigen-specific monoclonal antibodies has been developed to detect circulating immune complexes in patients infected with Toxocara canis. This technique could be used for the study of the dynamics of the parasite-host relationship, as we believe the detection of immune complexes and/or soluble antigen to be an improvement over detection of antibodies only. In this parasitosis, antibodies may be present in residual levels for prolonged periods after active infection.

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