scholarly journals The Microimmunofluorescence Test forChlamydia pneumoniaeInfection: Technique and Interpretation

2000 ◽  
Vol 181 (s3) ◽  
pp. S421-S425 ◽  
Author(s):  
San‐pin Wang
Keyword(s):  
Microimmunofluorescence Test

scholarly journals Psittacosis in a highly endemic area in Italy

1987 ◽  
Vol 99 (2) ◽  
pp. 413-419 ◽  
Author(s):  
C. Maffei ◽  
A. Marracino ◽  
F. Di Stanislao ◽  
P. Pauri ◽  
M. Clementi ◽  
...  
Keyword(s):  
Tract Infection ◽  
Lower Respiratory Tract ◽  
Endemic Area ◽  
Blood Donors ◽  
X Rays ◽  
Source Of Infection ◽  
Epidemiological Features ◽  
Probable Source ◽  
The University ◽  
Microimmunofluorescence Test

SUMMARYIn one locality in Italy where the incidence of psittacosis has increased rapidly since 1980, a hospital-based study and a seroepidemiological survey were carried out in order to define the clinical and epidemiological features of psittacosis in that area.Registers of the Virology Unit of the University of Ancona, Italy, were reviewed and all hospitalized patients with a serological diagnosis of psittacosis were identified. A total of 76 cases were found and studied. A presumptive bird source was identified in 80% of 62 patients, on whom a detailed investigation had been possible. Poultry represented the most frequent probable source of infection. Clinically, the predominant pattern of illness was a moderately severe lower respiratory tract infection, with chest X-rays showing pulmonary shadowings in 68 patients (89%).In the seroepidemiological study, 51 out of 143 subjects were exposed to birds (35·7%), but only 7 out of 90 urban adult blood donors (7·3%) were positive for chlamydial antibodies using the microimmunofluorescence test.

Assessment of the Microimmunofluorescence Test for Antibodies to Chlamydia trachomatis in Adults with Respiratory Disorders

1983 ◽  
Vol 148 (6) ◽  
pp. 1167-1168 ◽  
Author(s):  
J. N. Kurltsky ◽  
J. Schachter ◽  
C. V. Broome ◽  
H. W. Wilkinson ◽  
H. Wulff
Keyword(s):  
Chlamydia Trachomatis ◽  
Respiratory Disorders ◽  
Microimmunofluorescence Test

An Indirect Microimmunofluorescence Test for Detection of Chlamydial Antibodies in Ovine Fetal Fluids

1994 ◽  
Vol 6 (3) ◽  
pp. 315-320 ◽  
Author(s):  
T. P. Sanderson ◽  
A. A. Andersen ◽  
L. D. Miller ◽  
J. J. Andrew ◽  
B. H. Janke ◽  
...  
Keyword(s):  
Chlamydial Infection ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Rapid Test ◽  
Chlamydia Psittaci ◽  
Diagnostic Laboratory ◽  
Specific Antibodies ◽  
Ovine Fetal ◽  
Microimmunofluorescence Test ◽  
Fetal Fluids

The objective of this study was to evaluate an indirect microimmunofluorescence test (IMIF) for detection of Chlamydial antibodies in serum and/or thoracic fluids of aborted ovine fetuses. One hundred eighty-two ovine fetuses, including 64 fetuses from 40 ewes that were experimentally infected with an ovine abortion strain of Chlamydia psittaci at gestation days 90–100, 10 fetuses from 6 normal ewes, and 108 fetuses selected from those received at the Iowa Veterinary Diagnostic Laboratory, were evaluated in this study. Fetuses from experimentally infected ewes were examined 4–60 days after inoculation. The IMIF findings were compared with the results of complement fixation serology for Chlamydiae and concentrations of immunoglobulin (IgG). Chlamydiae-specific antibodies were detected by IMIF in 28 of 38 fetuses infected with C. psittaci. Elevated levels of IgG and IMIF titers ≥ 1:8 were consistent findings in ovine fetuses infected with Chlamydiae for more than 24 days. IgG levels and titers of Chlamydial antibodies increased with maturity of the fetus and duration of Chlamydial infection. Chlamydial antibodies were not detected with the complement fixation test. Fluids from ovine fetuses aborted as a result of other causes also were examined, and IMIF results were negative. The results of this study indicate that the IMIF is a useful and relatively rapid test for identification of Chlamydial antibodies in ovine fetuses.

scholarly journals Serotyping of US Isolates of Chlamydophila Psittaci from Domestic and Wild Birds

2005 ◽  
Vol 17 (5) ◽  
pp. 479-482 ◽  
Author(s):  
Arthur A. Andersen
Keyword(s):  
Monoclonal Antibodies ◽  
Disease Control ◽  
Rapid Method ◽  
Epidemiological Studies ◽  
Wild Birds ◽  
Disease Prognosis ◽  
Chlamydophila Psittaci ◽  
Microimmunofluorescence Test ◽  
Order Passeriformes

The identities of chlamydial strains, which can infect a given host, are important to know for disease prognosis, disease control, and epidemiology. The microimmunofluorescence test (MIFT) was used with a panel of 14 serovar-specific monoclonal antibodies (MAbs) to serotype 150 chlamydial isolates from domestic and wild birds. The isolates were obtained from birds submitted to diagnostic laboratories or during investigation of outbreaks. The 150 US isolates included 96 from the order Psittaciformes, 14 isolates from the order Columbiformes, 2 from the order Passeriformes, 16 from the order Galliformes, 12 from the order Struthioniformes, and 3 from the order Falconiformes. A total of 93, or 97%, of the Psittaciformes isolates were of serovar A; 11, or 79%, of the Columbiformes isolates were of serovar B; 64% of the Galliformes isolates were of serovar D, and all the Struthioniformes isolates were of serovar E. The 3 Falconiformes isolates did not react with any of the MAbs to the avian and mammalian isolates and are presumed to represent a new strain. The results show that specific chlamydial strains are usually associated with certain types of birds and that some serovars may be unusually virulent for certain species of birds. The MIFT using serovar-specific MAbs provides a rapid method to serotype new isolates, making it a useful system for epidemiological studies.

scholarly journals Seroprevalence of astrovirus types 1 and 6 in London, determined using recombinant virus antigen

1996 ◽  
Vol 117 (1) ◽  
pp. 159-164 ◽  
Author(s):  
S. Kriston ◽  
M. M. Willcocks ◽  
M. J. Carter ◽  
W. D. Cubitt
Keyword(s):  
Recombinant Virus ◽  
Age Groups ◽  
Recombinant Baculovirus ◽  
Virus Antigen ◽  
Capsid Proteins ◽  
Human Astrovirus ◽  
Microimmunofluorescence Test

SummaryWe have developed a microimmunofluorescence test (IF) which uses cells infected with a recombinant baculovirus which expresses the capsid proteins of astrovirus types 1 or 6. The IF test was sensitive and specific and the results for human astrovirus type 1 (HAst-1) were comparable to those obtained by immune electronmicroscopy and radioimmunoassay. Application of the test to a panel of 273 sera collected from patients and staff at two childrens hospitals in London showed that over 50% of the population were infected by Hast-1 between the age of 5 and 12 months rising to 90% by 5 years, whereas human astrovirus type 6 (HAst6) was relatively uncommon (10–30%) in all age groups.

scholarly journals Interlaboratory Reliability of Microimmunofluorescence Test for Measurement of Chlamydia pneumoniae-Specific Immunoglobulin A and G Antibody Titers

2004 ◽  
Vol 11 (3) ◽  
pp. 615-617 ◽  
Author(s):  
Alyson J. Littman ◽  
Lisa A. Jackson ◽  
Emily White ◽  
Mark D. Thornquist ◽  
Charlotte A. Gaydos ◽  
...  
Keyword(s):  
Measurement Error ◽  
Immunoglobulin G ◽  
Chlamydia Pneumoniae ◽  
Immunoglobulin A ◽  
Specific Immunoglobulin ◽  
Disease Associations ◽  
Antibody Titers ◽  
Iga Antibody ◽  
Microimmunofluorescence Test

ABSTRACT To evaluate the reliability of Chlamydia pneumoniae-specific immunoglobulin G (IgG) and IgA antibody titers as measured by the microimmunofluorescence (MIF) test, we compared results from 392 individuals using a standard MIF protocol at two academic laboratories. The kappas for dichotomous titers (≥16 versus <16) were 0.39 for IgA and 0.53 for IgG. Measurement error likely attenuates C. pneumoniae-disease associations; the magnitude of attenuation can be estimated from results of studies such as this one.

Simplified serological test for antibodies to Chlamydia trachomatis

1976 ◽  
Vol 4 (1) ◽  
pp. 6-10
Author(s):  
B J Thomas ◽  
P Reeve ◽  
J D Oriel
Keyword(s):  
Chlamydia Trachomatis ◽  
Serological Test ◽  
Venereal Disease ◽  
Preliminary Screening ◽  
Single Antigen ◽  
Chlamydial Antibody ◽  
Microimmunofluorescence Test

Three-hundred sixty sera from unselected patients attending two London venereal disease clinics were examined by a microimmunofluorescence test. Eleven egg-grown serotypes of Chlamydia trachomatis and the so-called "fast" strain SA2(f) were used as antigens. Of the 360 sera tested, 119 (33%) reacted to a titer of 1:16 or above with at least one antigen. Of these positive sera, over 50% cross-reacted with all 12 serotypes, and 95.5% reacted with SA2(f) in addition to other antigenic types. It is suggested that SA2(f) could be used as a single antigen for preliminary screening of a large number of sera for the presence or absence of chlamydial antibody.

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