scholarly journals Hepatocytes are Permissive for Human Cytomegalovirus Infection in Human Liver Cell Culture and In Vivo

1999 ◽  
Vol 180 (4) ◽  
pp. 976-986 ◽  
Author(s):  
C. Sinzger ◽  
A. L. Bissinger ◽  
R. Viebahn ◽  
H. Oettle ◽  
C. Radke ◽  
...  
Keyword(s):  
Cell Culture ◽  
Human Cytomegalovirus ◽  
Liver Cell ◽  
Human Liver ◽  
Cytomegalovirus Infection ◽  
Liver Cell Culture ◽  
Human Liver Cell ◽  
Human Cytomegalovirus Infection

Effects of porphyrin-inducing drugs on ferrochelatase activity in isolated mouse hepatocytes

1981 ◽  
Vol 59 (11) ◽  
pp. 1155-1158 ◽  
Author(s):  
Susan P. C. Cole ◽  
Thomas E. Massey ◽  
Gerald S. Marks ◽  
William J. Racz
Keyword(s):  
Cell Culture ◽  
Chick Embryo ◽  
Liver Cell ◽  
Mouse Hepatocyte ◽  
Liver Cell Culture ◽  
Embryo Liver ◽  
Mouse Hepatocytes ◽  
The Difference ◽  
Hepatocyte Suspension

The effects of several concentrations of griseofulvin and 3,5-diethoxycarbonyl-1,4-dihydro-2,4,6-trimethylpyridine (DDC) on ferrochelatase activity in suspensions of isolated mouse hepatocytes were examined. In agreement with previous findings in the intact chick embryo liver and chick embryo liver cell culture, DDC, but not griseofulvin, inhibited the enzyme in the isolated mouse hepatocyte suspension. These results indicate that the difference between the effects of griseofulvin on hepatic ferrochelatase in rodents in vivo (inhibition), the intact chick embryo (no effect), and the chick embryo liver cell culture (no effect) cannot be attributed solely to species differences.

scholarly journals Comparing in vitro human liver models to in vivo human liver using RNA-Seq

2020 ◽  
Author(s):  
Rajinder Gupta ◽  
Yannick Schrooders ◽  
Duncan Hauser ◽  
Marcel van Herwijnen ◽  
Wiebke Albrecht ◽  
...  
Keyword(s):  
Liver Cell ◽  
Liver Tissue ◽  
Hepg2 Cells ◽  
Human Liver ◽  
Rna Seq ◽  
Cell Models ◽  
Human Liver Tissue ◽  
Human Liver Cell

Abstract The liver plays an important role in xenobiotic metabolism and represents a primary target for toxic substances. Many different in vitro cell models have been developed in the past decades. In this study, we used RNA-sequencing (RNA-Seq) to analyze the following human in vitro liver cell models in comparison to human liver tissue: cancer-derived cell lines (HepG2, HepaRG 3D), induced pluripotent stem cell-derived hepatocyte-like cells (iPSC-HLCs), cancerous human liver-derived assays (hPCLiS, human precision cut liver slices), non-cancerous human liver-derived assays (PHH, primary human hepatocytes) and 3D liver microtissues. First, using CellNet, we analyzed whether these liver in vitro cell models were indeed classified as liver, based on their baseline expression profile and gene regulatory networks (GRN). More comprehensive analyses using non-differentially expressed genes (non-DEGs) and differential transcript usage (DTU) were applied to assess the coverage for important liver pathways. Through different analyses, we noticed that 3D liver microtissues exhibited a high similarity with in vivo liver, in terms of CellNet (C/T score: 0.98), non-DEGs (10,363) and pathway coverage (highest for 19 out of 20 liver specific pathways shown) at the beginning of the incubation period (0 h) followed by a decrease during long-term incubation for 168 and 336 h. PHH also showed a high degree of similarity with human liver tissue and allowed stable conditions for a short-term cultivation period of 24 h. Using the same metrics, HepG2 cells illustrated the lowest similarity (C/T: 0.51, non-DEGs: 5623, and pathways coverage: least for 7 out of 20) with human liver tissue. The HepG2 are widely used in hepatotoxicity studies, however, due to their lower similarity, they should be used with caution. HepaRG models, iPSC-HLCs, and hPCLiS ranged clearly behind microtissues and PHH but showed higher similarity to human liver tissue than HepG2 cells. In conclusion, this study offers a resource of RNA-Seq data of several biological replicates of human liver cell models in vitro compared to human liver tissue.

Glycoprotein B genotype correlates with cell tropism in vivo of human cytomegalovirus infection

1998 ◽  
Vol 55 (1) ◽  
pp. 75-81 ◽  
Author(s):  
Ursula Meyer-König ◽  
Christian Vogelberg ◽  
Angela Bongarts ◽  
Danielle Kampa ◽  
Regine Delbrück ◽  
...  
Keyword(s):  
Human Cytomegalovirus ◽  
Cytomegalovirus Infection ◽  
Glycoprotein B ◽  
Cell Tropism ◽  
Human Cytomegalovirus Infection

Human liver cell cultivation for long-term in vitro toxicity studies in a miniaturized multi-compartment 3D bioreactor system

2011 ◽  
Vol 49 (01) ◽  
Author(s):  
SA Hoffmann ◽  
M Lübberstedt ◽  
U Müller-Vieira ◽  
D Knobeloch ◽  
A Nüssler ◽  
...  
Keyword(s):  
Liver Cell ◽  
Human Liver ◽  
In Vitro Toxicity ◽  
Cell Cultivation ◽  
Toxicity Studies ◽  
Human Liver Cell ◽  
Bioreactor System
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