scholarly journals Cytokine Production in Acute versus Chronic Human Schistosomiasis Mansoni: The Cross‐Regulatory Role of Interferon‐γ and Interleukin‐10 in the Responses of Peripheral Blood Mononuclear Cells and Splenocytes to Parasite Antigens

1999 ◽  
Vol 179 (6) ◽  
pp. 1502-1514 ◽  
Author(s):  
Silvia M. L. Montenegro ◽  
Paulo Miranda ◽  
Siddhartha Mahanty ◽  
Frederico G. C. Abath ◽  
Kirte M. Teixeira ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Cytokine Production ◽  
Interleukin 10 ◽  
Interferon Γ ◽  
Peripheral Blood Mononuclear ◽  
Schistosomiasis Mansoni ◽  
Blood Mononuclear Cells

Defective Interleukin-10 Synthesis by Peripheral Blood Mononuclear Cells among Hemodialysis Patients

2002 ◽  
Vol 20 (6) ◽  
pp. 543-550 ◽  
Author(s):  
Mary C. Perianayagam ◽  
Bertrand L. Jaber ◽  
Daqing Guo ◽  
Andrew J. King ◽  
Brian J.G. Pereira ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Interleukin 10 ◽  
Hemodialysis Patients ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

scholarly journals Transcriptomic Profiling of Equine and Viral Genes in Peripheral Blood Mononuclear Cells in Horses during Equine Herpesvirus 1 Infection

Pathogens ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 43
Author(s):  
Lila M. Zarski ◽  
Patty Sue D. Weber ◽  
Yao Lee ◽  
Gisela Soboll Hussey
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Differentially Expressed ◽  
Equine Herpesvirus ◽  
Peripheral Blood Mononuclear ◽  
Equine Herpesvirus 1 ◽  
Blood Mononuclear Cells ◽  
Herpesvirus 1

Equine herpesvirus 1 (EHV-1) affects horses worldwide and causes respiratory disease, abortions, and equine herpesvirus myeloencephalopathy (EHM). Following infection, a cell-associated viremia is established in the peripheral blood mononuclear cells (PBMCs). This viremia is essential for transport of EHV-1 to secondary infection sites where subsequent immunopathology results in diseases such as abortion or EHM. Because of the central role of PBMCs in EHV-1 pathogenesis, our goal was to establish a gene expression analysis of host and equine herpesvirus genes during EHV-1 viremia using RNA sequencing. When comparing transcriptomes of PBMCs during peak viremia to those prior to EHV-1 infection, we found 51 differentially expressed equine genes (48 upregulated and 3 downregulated). After gene ontology analysis, processes such as the interferon defense response, response to chemokines, the complement protein activation cascade, cell adhesion, and coagulation were overrepresented during viremia. Additionally, transcripts for EHV-1, EHV-2, and EHV-5 were identified in pre- and post-EHV-1-infection samples. Looking at micro RNAs (miRNAs), 278 known equine miRNAs and 855 potentially novel equine miRNAs were identified in addition to 57 and 41 potentially novel miRNAs that mapped to the EHV-2 and EHV-5 genomes, respectively. Of those, 1 EHV-5 and 4 equine miRNAs were differentially expressed in PBMCs during viremia. In conclusion, this work expands our current knowledge about the role of PBMCs during EHV-1 viremia and will inform the focus on future experiments to identify host and viral factors that contribute to clinical EHM.

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