scholarly journals Interaction between Group A Streptococci and the Plasmin(ogen) System Promotes Virulence in a Mouse Skin Infection Model

1999 ◽  
Vol 179 (4) ◽  
pp. 907-914 ◽  
Author(s):  
Zhuqing Li ◽  
Victoria A. Ploplis ◽  
Esther L. French ◽  
Michael D. P. Boyle
Keyword(s):  
Skin Infection ◽  
Mouse Skin ◽  
Infection Model ◽  
Group A Streptococci ◽  
Group A

scholarly journals Biofilm/Persister/Stationary Phase Bacteria Cause More Severe Disease Than Log Phase Bacteria – II Infection with Persister Forms of Staphylococcus aureus Causes a Chronic Persistent Skin Infection with More Severe Lesion that Takes Longer to Heal and is not Eradicated by the Current Recommended Treatment in Mice

2018 ◽  
Author(s):  
Rebecca Yee ◽  
Yuting Yuan ◽  
Cory Brayton ◽  
Andreina Tarff Leal ◽  
Jie Feng ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Stationary Phase ◽  
Skin Infection ◽  
Bacterial Load ◽  
Mouse Skin ◽  
Infection Model ◽  
Persister Cells ◽  
Persistent Infections ◽  
Recommended Treatment ◽  
Biofilm Bacteria

AbstractStaphylococcus aureus is an opportunistic pathogen that can cause persistent infections clinically. Treatment for chronic S. aureus infections ranges from at least one week to several months and such infections are prone to relapse likely due to the presence of persistent forms of bacteria such as persister cells. Persister cells, which are bacterial cells that become dormant under stress conditions, can be isolated in vitro but their clinical significance in in vivo infections are largely unclear. Here, we evaluated S. aureus persistent forms using stationary phase cultures and biofilm bacteria (enriched in persisters) in comparison with log phase cultures in terms of their ability to cause disease in a mouse skin infection model. Surprisingly, we found that infection of mice with stationary phase cultures and biofilm bacteria produced a more severe chronic skin infection with more pronounced lesions which took longer to heal than log phase (actively growing) cultures. After two week infection, the bacterial load and skin tissue pathology, as determined by hyperplasia, immune cell infiltration, and crust/lesion formation, of mice infected with the more persistent forms (e.g. stationary phase bacteria and biofilm bacteria) were greater than mice infected with log phase bacteria. Using our persistent infection mouse model, we showed that the clinically recommended treatment for recurrent S. aureus skin infection, doxycycline + rifampin, was not effective in eradicating the bacteria in the treatment study, despite reducing lesion sizes and pathology in infected mice. Analogous findings were also observed in a Caenorhabditis elegans model, where S.aureus stationary phase cultures caused a greater mortality than log phase culture as early as two days post-infection. Thus, we established a new model for chronic persistent infections using persister bacteria that could serve as a relevant model to evaluate therapeutic options for persistent infections in general. Our findings connect persisters with persistent infections, have implications for understanding disease pathogenesis, and are likely to be broadly valid for other pathogens.

scholarly journals Intranasal Vaccination with Streptococcal Fibronectin Binding Protein Sfb1 Fails To Prevent Growth and Dissemination of Streptococcus pyogenes in a Murine Skin Infection Model

2004 ◽  
Vol 72 (12) ◽  
pp. 7342-7345 ◽  
Author(s):  
J. McArthur ◽  
E. Medina ◽  
A. Mueller ◽  
J. Chin ◽  
B. J. Currie ◽  
...  
Keyword(s):  
Streptococcus Pyogenes ◽  
Skin Infection ◽  
Group A Streptococcus ◽  
Binding Protein ◽  
Infection Model ◽  
Cholera Toxin B Subunit ◽  
Protective Antigens ◽  
Group A ◽  
Fibronectin Binding Protein ◽  
Fibronectin Binding

ABSTRACT Fibronectin binding protein F1 (Sfb1) of Streptococcus pyogenes (group A streptococcus [GAS]) is a well-characterized adhesin that has been shown to induce protection in mice against a lethal intranasal GAS challenge after intranasal immunization with cholera toxin B subunit (CTB) as adjuvant. With a murine skin infection model, we have shown that Sfb1/CTB vaccination neither elicits opsonizing antibodies nor prevents systemic bacterial growth and dissemination to internal organs after a subcutaneous GAS challenge. These results indicate that an Sfb1-based vaccine should be complemented with additional protective antigens in order to be used in areas such as the tropical north of Australia, where the skin is the primary route of entry for invasive streptococcal diseases.

scholarly journals Molecular typing ofStreptococcus pyogenesfrom remote Aboriginal communities where rheumatic fever is common and pyoderma is the predominant streptococcal infection

2007 ◽  
Vol 135 (8) ◽  
pp. 1398-1405 ◽  
Author(s):  
M. I. McDONALD ◽  
R. J. TOWERS ◽  
P. FAGAN ◽  
J. R. CARAPETIS ◽  
B. J. CURRIE
Keyword(s):  
Molecular Typing ◽  
Skin Infection ◽  
Streptococcal Infection ◽  
Group A Streptococci ◽  
Remote Communities ◽  
Aboriginal Communities ◽  
Aboriginal Australians ◽  
Pattern Type ◽  
Group A ◽  
Rheumatic Heart

SUMMARYAboriginal Australians in remote communities have high rates of rheumatic heart disease (RHD); yet pharyngitis is reportedly rare whilst pyoderma is common. Some strains of group A streptococci (GAS) have preference for the throat and others for the skin depending on M protein type. A study in three remote communities provided 350 GAS isolates foremmsequence typing, 244 were alsoemmpattern typed. There was 100% correlation betweenemmsequence and pattern type. Patterns D and E (non-throat tropic) made up 71% of throat and 87% of skin isolates although patterns A–C (throat tropic) were more common in the throat than the skin (RR 2·3, 95% CI 1·4–3·8) whilst the opposite was found for pattern D (RR 2·2, 95% CI 1·7–3·0). Pattern E favoured the throat (RR 1·4, 95% CI 1·1–1·8). Where environmental factors predispose to skin infection,emmpattern types D and E prevail, whatever the recovery site.

Adherence and fibronectin binding are environmentally regulated in the group A streptococci

1993 ◽  
Vol 9 (6) ◽  
pp. 1213-1222 ◽  
Author(s):  
Tambryn VanHeyningen ◽  
George Fogg ◽  
Debra Yates ◽  
Emanuel Hanski ◽  
Michael Caparon
Keyword(s):  
Group A Streptococci ◽  
Group A ◽  
Fibronectin Binding

Kinetics of Serum Cytokine Profile in Mice after Injection of Supernatants of Group A Streptococci Culture

2019 ◽  
Vol 167 (3) ◽  
pp. 367-370 ◽  
Author(s):  
Т. А. Danilova ◽  
G. А. Danilina ◽  
А. А Аdzhieva ◽  
A. G. Minko
Keyword(s):  
Cytokine Profile ◽  
Group A Streptococci ◽  
Serum Cytokine ◽  
Group A ◽  
Kinetics Of
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