scholarly journals Highly Active Antiretroviral Therapy and Progressive Multifocal Leukoencephalopathy: Effects on Cerebrospinal Fluid Markers of JC Virus Replication and Immune Response

2000 ◽  
Vol 30 (1) ◽  
pp. 95-99 ◽  
Author(s):  
B. Giudici ◽  
B. Vaz ◽  
S. Bossolasco ◽  
S. Casari ◽  
A. M. Brambilla ◽  
...  
Keyword(s):  
Immune Response ◽  
Cerebrospinal Fluid ◽  
Antiretroviral Therapy ◽  
Progressive Multifocal Leukoencephalopathy ◽  
Virus Replication ◽  
Highly Active Antiretroviral Therapy ◽  
Jc Virus ◽  
Highly Active

scholarly journals Dual Qualitative-Quantitative Nested PCR for Detection of JC Virus in Cerebrospinal Fluid: High Potential for Evaluation and Monitoring of Progressive Multifocal Leukoencephalopathy in AIDS Patients Receiving Highly Active Antiretroviral Therapy

1999 ◽  
Vol 37 (3) ◽  
pp. 724-728 ◽  
Author(s):  
Darío García de Viedma ◽  
Roberto Alonso ◽  
Pilar Miralles ◽  
Juan Berenguer ◽  
Marta Rodriguez-Créixems ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Antiretroviral Therapy ◽  
Progressive Multifocal Leukoencephalopathy ◽  
Nested Pcr ◽  
Highly Active Antiretroviral Therapy ◽  
Control Group ◽  
Analytical Sensitivity ◽  
Aids Patients ◽  
Term Survival ◽  
Highly Active

JC polyomavirus (JCV) is the causative agent of progressive multifocal leukoencephalopathy (PML), a central nervous system infection that mainly affects AIDS patients. The extensive application of highly active antiretroviral therapy (HAART) is leading to the appearance of “long-term” survival PML patients. A reliable and feasible qualitative-quantitative test for both the detection of JCV and follow-up of its viral burden in this emerging group of patients is clearly required. With this aim, a dual qualitative-quantitative nested PCR is presented in this study for the analysis of JCV DNA in cerebrospinal fluid (CSF). Two newly designed internal controls, one competitive and the other noncompetitive, have been constructed to adapt this PCR to either measure the JCV burden or to allow a highly confident determination of JCV presence or clearance. The analytical sensitivity of the technique allows the detection of 0.01 fg (three genomes) of JCV DNA. Its qualitative application has been evaluated by analyzing single CSF samples from a group of 17 patients with PML and a control group of 20 patients with diverse neurological conditions other than PML, yielding sensitivity and specificity values of 100 and 90%, respectively. The quantitative application has been evaluated in vitro in blind tests with samples including serial dilutions of JCV, and in all cases the samples were successfully ordered considering the JCV titer. The dual quantitative-qualitative application offered by this nested PCR may provide an answer to the new requirements for evaluating and finely monitoring PML in AIDS patients receiving HAART.

Characterization of the HIV-1 Specific Humoral Immune Response During Highly Active Antiretroviral Therapy (HAART)

2001 ◽  
Vol 28 (5) ◽  
pp. 405-415 ◽  
Author(s):  
Mary Kate Morris ◽  
David A. Katzenstein ◽  
Dennis Israelski ◽  
Andrew Zolopa ◽  
R. Michael Hendry ◽  
...  
Keyword(s):  
Immune Response ◽  
Antiretroviral Therapy ◽  
Humoral Immune Response ◽  
Highly Active Antiretroviral Therapy ◽  
Humoral Immune ◽  
Highly Active ◽  
Hiv 1

scholarly journals Pediatric Immune Response Syndrome after Initiation of Highly Active Antiretroviral Therapy

1999 ◽  
Vol 45 (4, Part 2 of 2) ◽  
pp. 159A-159A
Author(s):  
Daniel H Conway ◽  
Mark L Bagarazzi ◽  
Kelly Hassey ◽  
Audrey S Kamrin ◽  
Harold W Lischner
Keyword(s):  
Immune Response ◽  
Antiretroviral Therapy ◽  
Highly Active Antiretroviral Therapy ◽  
Highly Active

Highly active antiretroviral therapy significantly improves the prognosis of patients with HIV-associated progressive multifocal leukoencephalopathy

AIDS ◽  
1998 ◽  
Vol 12 (10) ◽  
pp. 1149-1154 ◽  
Author(s):  
Helmut Albrecht ◽  
Christian Hoffmann ◽  
Olaf Degen ◽  
Albrecht Stoehr ◽  
Andreas Plettenberg ◽  
...  
Keyword(s):  
Antiretroviral Therapy ◽  
Progressive Multifocal Leukoencephalopathy ◽  
Highly Active Antiretroviral Therapy ◽  
Highly Active

Inflammatory reactions in progressive multifocal leukoencephalopathy after highly active antiretroviral therapy

AIDS ◽  
2001 ◽  
Vol 15 (14) ◽  
pp. 1900-1902 ◽  
Author(s):  
Pilar Miralles ◽  
Juan Berenguer ◽  
Cesar Lacruz ◽  
Jaime Cosín ◽  
Juan Carlos López ◽  
...  
Keyword(s):  
Antiretroviral Therapy ◽  
Progressive Multifocal Leukoencephalopathy ◽  
Highly Active Antiretroviral Therapy ◽  
Inflammatory Reactions ◽  
Highly Active
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