scholarly journals Genetic Basis of Peroxisome-Assembly Mutants of Humans, Chinese Hamster Ovary Cells, and Yeast: Identification of a New Complementation Group of Peroxisome-Biogenesis Disorders Apparently Lacking Peroxisomal-Membrane Ghosts

1998 ◽  
Vol 63 (6) ◽  
pp. 1898-1906 ◽  
Author(s):  
Nobuyuki Shimozawa ◽  
Yasuyuki Suzuki ◽  
Zhongyi Zhang ◽  
Atsushi Imamura ◽  
Naomi Kondo ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Genetic Basis ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Complementation Group ◽  
Peroxisome Biogenesis ◽  
Yeast Identification ◽  
Peroxisomal Membrane ◽  
Ovary Cells ◽  
Peroxisome Biogenesis Disorders

scholarly journals Selection of specific wheat germ agglutinin-resistant (WgaR) phenotypes from Chinese hamster ovary cell populations containing numerous lecR genotypes.

1981 ◽  
Vol 1 (8) ◽  
pp. 687-696 ◽  
Author(s):  
P Stanley
Keyword(s):  
Chinese Hamster Ovary ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Complementation Group ◽  
Ovary Cell ◽  
Mutant Type ◽  
Similar Frequency ◽  
Ovary Cells

Three distinct Chinese hamster ovary mutants selected for resistance to wheat germ agglutinin were previously described by this laboratory. In this paper, evidence is provided that each phenotype occurs at a similar frequency in an unmutagenized population of Chinese hamster ovary cells. Two novel wheat germ agglutinin resistance phenotypes (WgaR), which also appear to occur at similar frequencies were uncovered in the course of these studies. One mutant type belongs to a new, recessive complementation group (VIII), and the second belongs to a previously defined complementation group (VI). Mutants from each of the four WgaR complementation groups (I, II, III, and VIII) exhibited characteristic and unique patterns of resistance to the toxicity of a variety of plant lectins. These properties were used in developing independent selection protocols which were highly specific for the isolation of each of the mutant types.

scholarly journals Identification and characterization of a third complementation group of emetine-resistant Chinese hamster cell mutants.

1981 ◽  
Vol 1 (1) ◽  
pp. 58-65 ◽  
Author(s):  
J J Wasmuth ◽  
J M Hill ◽  
L S Vock
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Chinese Hamster Cell ◽  
Resistant Cell ◽  
Complementation Group ◽  
Ovary Cells ◽  
Complementation Groups ◽  
Resistant Mutants ◽  
Biosynthetic Machinery

We have isolated emetine-resistant cell lines from Chinese hamster peritoneal fibroblasts and have shown that they represent a third distinct class or complementation group of emetine-resistant mutants, as determined by three different criteria. These mutants, like those belonging to the two other complementation groups we have previously defined, which were isolated from Chinese hamster lung and Chinese hamster ovary cells, have alterations that directly affect the protein biosynthetic machinery. So far, there is absolute cell line specificity with respect to the three complementation groups, in that all the emetine-resistant mutants we have isolated from Chinese hamster lung cells belong to one complementation group, all those we have isolated from Chinese hamster ovary cells belong to a second complementation group, and all those isolated from Chinese hamster peritoneal cells belong to a third complementation group. Thus, in cultured Chinese hamster cells, mutations in at least three different loci, designated emtA, emtB, and emtC, encoding for different components of the protein biosynthetic machinery, can give rise to the emetine-resistant phenotype.

Selection of specific wheat germ agglutinin-resistant (WgaR) phenotypes from Chinese hamster ovary cell populations containing numerous lecR genotypes

1981 ◽  
Vol 1 (8) ◽  
pp. 687-696
Author(s):  
P Stanley
Keyword(s):  
Chinese Hamster Ovary ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Complementation Group ◽  
Ovary Cell ◽  
Mutant Type ◽  
Similar Frequency ◽  
Ovary Cells

Three distinct Chinese hamster ovary mutants selected for resistance to wheat germ agglutinin were previously described by this laboratory. In this paper, evidence is provided that each phenotype occurs at a similar frequency in an unmutagenized population of Chinese hamster ovary cells. Two novel wheat germ agglutinin resistance phenotypes (WgaR), which also appear to occur at similar frequencies were uncovered in the course of these studies. One mutant type belongs to a new, recessive complementation group (VIII), and the second belongs to a previously defined complementation group (VI). Mutants from each of the four WgaR complementation groups (I, II, III, and VIII) exhibited characteristic and unique patterns of resistance to the toxicity of a variety of plant lectins. These properties were used in developing independent selection protocols which were highly specific for the isolation of each of the mutant types.

scholarly journals Impaired lysosomes in a temperature-sensitive mutant of Chinese hamster ovary cells.

1989 ◽  
Vol 108 (6) ◽  
pp. 2211-2219 ◽  
Author(s):  
P A Colbaugh ◽  
M Stookey ◽  
R K Draper
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Complementation Group ◽  
Temperature Sensitive ◽  
Restrictive Temperature ◽  
Ovary Cells ◽  
Acidification Activity ◽  
Protein Toxins ◽  
Beta Galactosidase

We describe here the properties of a mutant of Chinese hamster ovary cells that expresses a conditional-lethal mutation affecting dense lysosomes. This mutant, termed V.24.1, is a member of the End4 complementation group of temperature-sensitive mutants selected for resistance to protein toxins (Colbaugh, P. A., C.-Y. Kao, S.-P. Shia, M. Stookey, and R. K. Draper. 1988. Somatic Cell Mol. Genet. 14:499-507). Vesicles present in postnuclear supernatants prepared from V.24.1 cells harvested at the restrictive temperature had a 50% reduction in acidification activity, assessed by the ATP-stimulated accumulation of the dye acridine orange in acidic vesicles. To investigate whether specific populations of vesicles were impaired in acidification, we measured acidification activity in three subcellular fractions prepared from Percoll gradients: one containing endosomal and Golgi markers, one containing buoyant lysosomes, and the third containing dense lysosomes. Activity in dense lysosomes was reduced by 90%, activity in the buoyant lysosome fraction was unaffected, and activity in the endosome-Golgi fraction was mildly reduced. The activity of three lysosomal enzymes--beta-hexosaminidase, beta-galactosidase, and beta-glucocerebrosidase--was also reduced in dense lysosomes but nearly normal in the buoyant lysosome fraction. However, beta-hexosaminidase and beta-glucocerebrosidase activity was increased two- to threefold in the endosome-Golgi fraction. We conclude that the lesion selectively impairs dense lysosomes but has little effect on properties of buoyant lysosomes.

Identification and characterization of a third complementation group of emetine-resistant Chinese hamster cell mutants

1981 ◽  
Vol 1 (1) ◽  
pp. 58-65
Author(s):  
J J Wasmuth ◽  
J M Hill ◽  
L S Vock
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Chinese Hamster Cell ◽  
Resistant Cell ◽  
Complementation Group ◽  
Ovary Cells ◽  
Complementation Groups ◽  
Resistant Mutants ◽  
Biosynthetic Machinery

We have isolated emetine-resistant cell lines from Chinese hamster peritoneal fibroblasts and have shown that they represent a third distinct class or complementation group of emetine-resistant mutants, as determined by three different criteria. These mutants, like those belonging to the two other complementation groups we have previously defined, which were isolated from Chinese hamster lung and Chinese hamster ovary cells, have alterations that directly affect the protein biosynthetic machinery. So far, there is absolute cell line specificity with respect to the three complementation groups, in that all the emetine-resistant mutants we have isolated from Chinese hamster lung cells belong to one complementation group, all those we have isolated from Chinese hamster ovary cells belong to a second complementation group, and all those isolated from Chinese hamster peritoneal cells belong to a third complementation group. Thus, in cultured Chinese hamster cells, mutations in at least three different loci, designated emtA, emtB, and emtC, encoding for different components of the protein biosynthetic machinery, can give rise to the emetine-resistant phenotype.

Malignancy-related characteristics of wild type and drug-resistant chinese hamster ovary cells

Pathology ◽  
1993 ◽  
Vol 25 (3) ◽  
pp. 268-276 ◽  
Author(s):  
Wanda B. Mackinnon ◽  
Marlen Dyne ◽  
Rebecca Hancock ◽  
Carolyn E. Mountford ◽  
Adrienne J. Grant ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Drug Resistant ◽  
Wild Type ◽  
Ovary Cells

Review of the current methods of Chinese Hamster Ovary (CHO) cells cultivation for production of therapeutic protein

2020 ◽  
Vol 17 ◽  
Author(s):  
Shazid Md. Sharker ◽  
Md. Atiqur Rahman
Keyword(s):  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Mass Production ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Therapeutic Protein ◽  
Specific Productivity ◽  
Ovary Cells ◽  
Further Development ◽  
Interesting Area

Most of clinical approved protein-based drugs or under in clinical trial have a profound impact in the treatment of critical diseases. The mammalian eukaryotic cells culture approaches, particularly the CHO (Chinese Hamster Ovary) cells are mainly used in the biopharmaceutical industry for the mass-production of therapeutic protein. Recent advances in CHO cell bioprocessing to yield recombinant proteins and monoclonal antibodies have enabled the expression of quality protein. The developments of cell lines are possible to upgrade specific productivity. As a result, it holds an interesting area for academic as well as industrial researchers around the world. This review will concentrate on the recent progress of the mammalian CHO cells culture technology and the future scope of further development for the mass-production of protein therapeutics.

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