scholarly journals From Amplification to Gene in Thyroid Cancer: A High-Resolution Mapped Bacterial-Artificial-Chromosome Resource for Cancer Chromosome Aberrations Guides Gene Discovery after Comparative Genome Hybridization

1998 ◽  
Vol 63 (2) ◽  
pp. 625-637 ◽  
Author(s):  
X.-N. Chen ◽  
J.A. Knauf ◽  
R. Gonsky ◽  
M. Wang ◽  
E.H. Lai ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
High Resolution ◽  
Bacterial Artificial Chromosome ◽  
Chromosome Aberrations ◽  
Gene Discovery ◽  
Artificial Chromosome ◽  
Comparative Genome Hybridization ◽  
Comparative Genome

scholarly journals High-Resolution Physical Map of theSinorhizobium meliloti 1021 pSyma Megaplasmid

2000 ◽  
Vol 182 (4) ◽  
pp. 1185-1189 ◽  
Author(s):  
Frederique Barloy-Hubler ◽  
Delphine Capela ◽  
Melanie J. Barnett ◽  
Sue Kalman ◽  
Nancy A. Federspiel ◽  
...  
Keyword(s):  
High Resolution ◽  
Bacterial Artificial Chromosome ◽  
Sinorhizobium Meliloti ◽  
Physical Map ◽  
Artificial Chromosome ◽  
Sequence Tagged Site ◽  
High Resolution Map ◽  
Site Markers ◽  
Resolution Map

ABSTRACT To facilitate sequencing of the Sinorhizobium meliloti1021 pSyma megaplasmid, a high-resolution map was constructed by ordering 113 overlapping bacterial artificial chromosome clones with 192 markers. The 157 anonymous sequence tagged site markers (81,072 bases) reveal hypothetical functions encoded by the replicon.

Assembly of High-Resolution Bacterial Artificial Chromosome, P1-Derived Artificial Chromosome, and Cosmid Contigs

1995 ◽  
Vol 224 (2) ◽  
pp. 564-571 ◽  
Author(s):  
L.K. Ashworth ◽  
M. Alegriahartman ◽  
M. Burgin ◽  
L. Devlin ◽  
A.V. Carrano ◽  
...  
Keyword(s):  
High Resolution ◽  
Bacterial Artificial Chromosome ◽  
Artificial Chromosome ◽  
Cosmid Contigs

High Resolution Genome-Wide Array-Based Comparative Genome Hybridization Reveals Cryptic Chromosome Changes in AML and MDS Cases with Trisomy 8 as the Sole Cytogenetic Aberration.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 2847-2847
Author(s):  
Kajsa Paulsson ◽  
Markus Heidenblad ◽  
Bodil Strombeck ◽  
Ake Borg ◽  
Thoas Fioretos ◽  
...  
Keyword(s):  
High Resolution ◽  
Array Cgh ◽  
Comparative Genome Hybridization ◽  
Trisomy 8 ◽  
Genetic Event ◽  
Comparative Genome ◽  
Cytogenetic Aberration ◽  
Hemizygous Deletion ◽  
Genome Wide ◽  
Genetic Abnormalities

Abstract Although trisomy 8 as the sole chromosome aberration is the most common numerical abnormality in acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS), next to nothing is known about its pathogenetic effects. Considering that +8 is a frequent secondary change in AML and MDS, cryptic - and possibly primary - genetic aberrations may occur in cases with trisomy 8 as the apparently single anomaly. To date, however, no such hidden anomalies have been reported. We performed a high resolution genome-wide array-based comparative genome hybridization (array CGH) analysis of ten AML/MDS cases with isolated +8, using an array set containing >30,000 BAC and PAC clones. Array CGH revealed intra-chromosomal imbalances, not corresponding to known genomic polymorphisms, in 5/10 cases, comprising ten duplications and hemizygous deletions ranging in size from 0.5 to 2.2 Mb. Most notably, a 1.8 Mb hemizygous deletion at 7p14.1, which had occurred prior to the +8, was identified in one MDS transforming to AML. Furthermore, a hemizygous deletion at 12p13.2, including ETV6, was present in one case. The remaining eight imbalances involved more than 40 genes. The present results show that cryptic genetic abnormalities are frequent in trisomy 8-positive AML and MDS cases and that +8 as the sole cytogenetic aberration is not always the primary genetic event.

Application of Array-based Comparative Genome Hybridization in patients with mental retardation

2010 ◽  
Vol 41 (02) ◽  
Author(s):  
K Storm van's Gravesande ◽  
M Linder-Lucht ◽  
U Köhler ◽  
R Korinthenberg ◽  
H Omran
Keyword(s):  
Mental Retardation ◽  
Comparative Genome Hybridization ◽  
Comparative Genome

The experience of holding the cycles of assisted reproductive technology with defrost, a biopsy, genetic study and refreezing of embryos in patients with multiple unsuccessful implantations

2016 ◽  
pp. 166-170
Author(s):  
Y.V. Masliy ◽  
◽  
I.O. Sudoma ◽  
P.S. Mazur ◽  
D.A. Mykytenko ◽  
...  
Keyword(s):  
Chromosomal Rearrangements ◽  
Genetic Diagnosis ◽  
Morphological Characteristics ◽  
Implantation Rate ◽  
Ovarian Response ◽  
Reproductive Technologies ◽  
Comparative Genome Hybridization ◽  
Comparative Genome ◽  
Vitro Fertilization

The objective: to study the possibility of using frozen blastocysts for biopsy and genetic testing and performance measurement transfer euploeded 5–7-day-old embryos after thawing, biopsies, refreezing and thawing in patients with unsuccessful implantation. Patients and methods. The object of the study was the group of patients with repeated failure of implantation (4) in programs of auxiliary reproductive technologies (ART), subject to transfer to the uterus in total (i.e. in all the programs) for at least 6 good quality embryos based on morphological characteristics). All women had sufficient ovarian reserve. The patient was treated for infertility within the ART programs of the clinic of reproductive medicine "Nadiya" in the period from 2006 to 2016. The sample included couples who were not carriers of chromosomal rearrangements, without anomalies of the uterus (congenital and acquired: a doubling of the uterus, one-horned uterus, intrauterine membrane, synechia, submucous myoma of the uterus). All women had a positive ovarian response to controlled stimulation with gonadotropins (at least 7 oocytes) and a sufficient number of cryopreserved embryos. The first group (G1) included 64 women who trophectodermal a biopsy was performed on fresh blastocysts (in a loop controlled ovarian hyperstimulation). The second group (G2) were included 31 women who underwent thawing previously cryopreserved blastocysts trophectodermal re-biopsy and vitrification of blastocysts. Results. It was found that the performance of transfers euploid embryos that were vitrified, bioptrone and revitriphted, a little lower than those that were bioptrone fresh and vitrified only once. At the same time computationa genetic diagnosis previously vitrified blastocysts using comparative genome hybridization in patients with recurrent failed implantation allows to obtain a reasonable pregnancy rate (58%), implantation rate (33.3 %) and the birth of living children (45.1 %). Conclusion. Reprising biopropane embryos does not cause significant destructive impact and allows you to achieve pregnancy and birth of the alive child. Key words: in vitro fertilization, reusable unsuccessful implantation, a method of comparative genome hybridization, refreezing.

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