Estimation of the Thermal Niche of Drosophila melanogaster Using a Temperature-Sensitive Mutation

1987 ◽  
Vol 130 (1) ◽  
pp. 83-90 ◽  
Author(s):  
J. S. Jones ◽  
Jerry A. Coyne ◽  
Linda Partridge
Keyword(s):  
Drosophila Melanogaster ◽  
Temperature Sensitive ◽  
Thermal Niche ◽  
Temperature Sensitive Mutation

Genetic analysis of vitellogenesis in Drosophila melanogaster: the identification of a temperature-sensitive mutation affecting one of the yolk proteins

Development ◽  
1978 ◽  
Vol 47 (1) ◽  
pp. 111-120
Author(s):  
M. Bownes ◽  
B. D. Hames
Keyword(s):  
Drosophila Melanogaster ◽  
Genetic Analysis ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Temperature Sensitive ◽  
Large Reduction ◽  
Yolk Proteins ◽  
Protein Uptake ◽  
Temperature Sensitive Mutation ◽  
Transplant Experiments

A number of female sterile mutations on the first and third chromosomes of Drosophila melanogaster have been screened for defects in the yolk proteins using polyacrylamide gel electrophoresis. Two new mutants were identified. 6m45 accumulates all three yolk proteins (YP1, YP2 and YP3) in the haemolymph but they are all absent from the ovaries suggesting it is a yolk-protein-uptake mutant. In contrast, 1163 is a temperature-sensitive mutation with a large reduction in the quantity of YP1 in the haemolymph and ovaries at 29 °C. Both mutants are autonomous in ovary transplant experiments.

scholarly journals Reversible alteration in the neuromuscular junctions of Drosophila melanogaster bearing a temperature-sensitive mutation, shibire.

1979 ◽  
Vol 81 (3) ◽  
pp. 520-527 ◽  
Author(s):  
C A Poodry ◽  
L Edgar
Keyword(s):  
Drosophila Melanogaster ◽  
Temperature Change ◽  
Synaptic Vesicles ◽  
Neuromuscular Junctions ◽  
Prior Treatment ◽  
Temperature Sensitive ◽  
Large Numbers ◽  
Temperature Sensitive Mutation

In this study we report a relationship between the ultrastruct of the neuromuscular junctions of tibial muscles and the temperature-induced paralysis in shibire flies. There is a decrease in the number of synaptic vesicles of neuromuscular junctions in flies which are held at or above 29 degrees. Shortly after return to 22 degrees C, the synaptic vesicles are again present in large numbers. Prior treatment with tetrodotoxin or barbiturate protects the junctions from the temperature change in morphology.

scholarly journals THE MECHANISM OF A BROODING EFFECT ASSOCIATED WITH SEGREGATION DISTORTION IN DROSOPHILA MELANOGASTER

Genetics ◽  
1973 ◽  
Vol 74 (4) ◽  
pp. 619-631
Author(s):  
D L Hartl
Keyword(s):  
Drosophila Melanogaster ◽  
Segregation Distortion ◽  
Mature Sperm ◽  
Temperature Sensitive ◽  
Short Time

ABSTRACT The recovery of the SD chromosome from a heterozygous SD male increases with brood. This is independent of the age of the female, occurs during the time the sperm are stored in the females, disappears when the segregation distortion is suppressed, and is temperature-sensitive-temperature shocks above or below 25°C applied to the mature sperm both tend to accelerate the increase in the recovery of SD. All this suggests the existence of a class of sperm affected by SD in which the sperm are able to fertilize eggs for a short time following ejaculation but become dysfunctional thereafter.

Genetic exploration of interactive domains in RNA polymerase II subunits

1990 ◽  
Vol 10 (5) ◽  
pp. 1908-1914
Author(s):  
C Martin ◽  
S Okamura ◽  
R Young
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Temperature Sensitive ◽  
Suppressor Mutations ◽  
Region I ◽  
Temperature Sensitive Mutation ◽  
Allele Specific ◽  
Separate Protein

The two large subunits of RNA polymerase II, RPB1 and RPB2, contain regions of extensive homology to the two large subunits of Escherichia coli RNA polymerase. These homologous regions may represent separate protein domains with unique functions. We investigated whether suppressor genetics could provide evidence for interactions between specific segments of RPB1 and RPB2 in Saccharomyces cerevisiae. A plasmid shuffle method was used to screen thoroughly for mutations in RPB2 that suppress a temperature-sensitive mutation, rpb1-1, which is located in region H of RPB1. All six RPB2 mutations that suppress rpb1-1 were clustered in region I of RPB2. The location of these mutations and the observation that they were allele specific for suppression of rpb1-1 suggests an interaction between region H of RPB1 and region I of RPB2. A similar experiment was done to isolate and map mutations in RPB1 that suppress a temperature-sensitive mutation, rpb2-2, which occurs in region I of RPB2. These suppressor mutations were not clustered in a particular region. Thus, fine structure suppressor genetics can provide evidence for interactions between specific segments of two proteins, but the results of this type of analysis can depend on the conditional mutation to be suppressed.

scholarly journals Characterization of a temperature-sensitive mutation that impairs the function of yeast tRNA nucleotidyltransferase

Yeast ◽  
2008 ◽  
Vol 25 (3) ◽  
pp. 219-233 ◽  
Author(s):  
Xunying Shan ◽  
Tara A. Russell ◽  
Scott M. Paul ◽  
David B. Kushner ◽  
Paul B. M. Joyce
Keyword(s):  
Temperature Sensitive ◽  
Yeast Trna ◽  
Temperature Sensitive Mutation
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