scholarly journals Mode of Action of Chronotropic Agents in Cardiac Purkinje Fibers

1974 ◽  
Vol 64 (3) ◽  
pp. 320-342 ◽  
Author(s):  
Richard W. Tsien
Keyword(s):  
Surface Charge ◽  
External Surface ◽  
Membrane Surface ◽  
Internal Surface ◽  
Phosphodiesterase Inhibitors ◽  
Common Mechanism ◽  
Pacemaker Activity ◽  
Response Curves ◽  
Voltage Shift ◽  
Cardiac Purkinje Fibers

Hauswirth et al. (1968) proposed that epinephrine acts on iKK2 by adding its own positive charge to the external membrane surface near the iKK2 channel. This hypothesis was tested by using noncationic compounds, theophylline and R07-2956, which mimicked epinephrine's effects on pacemaker activity and on iKK2. In maximally effective doses, theophylline or R07-2956 occluded the effect of epinephrine, indicating a shared final common mechanism. Since theophylline and R07-2956 are noncationic at pH 7.4, the common mechanism cannot be a direct change in external surface charge. On the contrary, epinephrine does not interfere with the voltage shift produced by La+++, which is thought to modify the external surface charge. The results argue against the original hypothesis but leave open the possibility that an alteration in internal surface charge generates the observed voltage shift. The potency of theophylline and R07-2956 as phosphodiesterase inhibitors suggests that the final common mechanism begins with the elevation of intracellular cyclic AMP, leading to a saturable process which limits the voltage shift's magnitude. This hypothesis is used to generate dose-response curves describing the combined effects of epinephrine and theophylline, and these are compared with experimental data.

scholarly journals Effects of Epinephrine on the Pacemaker Potassium Current of Cardiac Purkinje Fibers

1974 ◽  
Vol 64 (3) ◽  
pp. 293-319 ◽  
Author(s):  
Richard W. Tsien
Keyword(s):  
Potassium Current ◽  
Rate Coefficient ◽  
Dose Dependence ◽  
Pacemaker Activity ◽  
Maximal Effect ◽  
Activation Curve ◽  
Voltage Shift ◽  
Purkinje Fibers ◽  
Cardiac Purkinje Fibers ◽  
Order Of Magnitude

Epinephrine promotes spontaneous activity in cardiac Purkinje fibers through its action on the pacemaker potassium current (iKK2). The mechanism of the acceleratory effect was studied by means of a voltage clamp technique. The results showed that the hormone speeds the deactivation of iKK2 during pacemaker activity by displacing the kinetic parameters of iKK2 toward less negative potentials. This depolarizing voltage shift is the sole explanation of the acceleratory effect since epinephrine did not alter the rectifier properties of iKK2, or the underlying inward leakage current, or the threshold for iNNa. The dose dependence of the voltage shift in the iKK2 activation curve was similar in 1.8 and 5.4 mM [Ca]o. The maximal voltage shift (usually ∼20 mV) was produced by epinephrine concentrations of > 10-6 M. The half-maximal effect was evoked by 60 nM epinephrine, nearly an order of magnitude lower than required for half-maximal effect on the secondary inward current (Carmeliet and Vereecke, 1969). The ß-blocker propranolol (10-6 M) prevented the effect of epinephrine (10-7M) but by itself gave no voltage shift. Epinephrine shifted the activation rate coefficient α8 to a greater extent than the deactivation rate coefficient ß8, and often steepened the voltage dependence of the steady-state activation curve. These deviations from simple voltage shift behavior were discussed in terms of possible mechanisms of epinephrine's action on the iKK2 channel.

Surface properties and clay mineralogy of hydrated halloysitic soil clays. I: Existence of interlayer K+ specific sites

Clay Minerals ◽  
1990 ◽  
Vol 25 (2) ◽  
pp. 129-139 ◽  
Author(s):  
B. Delvaux ◽  
A. J. Herbillon ◽  
J. E. Dufey ◽  
L. Vielvoye
Keyword(s):  
Adsorption Isotherm ◽  
Surface Charge ◽  
Surface Properties ◽  
External Surface ◽  
Chloride Concentration ◽  
Clay Mineralogy ◽  
Internal Surface ◽  
Surface Areas ◽  
Soil Clays ◽  
Tropical Conditions

AbstractSix deferrated clay (<2 µm) samples from the major B horizons of pedons derived from basaltic pyroclasts (Western Cameroon) were selected. These soil clays, which differ mainly in their contents of halloysite and kaolinite, represent the types of secondary constituents characteristic of different weathering stages in soils derived from such volcanic materials under humid tropical conditions. On these clays, Ca2+-K+ exchange equilibria were performed at pH 6 and 0·01 n chloride concentration. The amounts of K+ specific sites were estimated through a two-site modelling of the exchange isotherm curves. The total and external surface areas of the clays were measured by EGME retention and by the BET treatment of the N2 adsorption isotherm, respectively. Relative halloysite content was found to be directly related to all measured surface charge properties, i.e. CEC, amount of K+ specific sites, and total and internal surface areas. It was also observed that thermal collapse of halloysitic layers reduced both the CEC and the amount of K+ specific sites. All the surface properties observed point to the presence of smectite units intimately associated with halloysite in these clays. The results further suggest that the layer charge of these smectite units differs in different samples.

Highly proton conductive membranes based on carboxylated cellulose nanofibres and their performance in proton exchange membrane fuel cells

2019 ◽  
Author(s):  
Valentina Guccini ◽  
Annika Carlson ◽  
Shun Yu ◽  
Göran Lindbergh ◽  
Rakel Wreland Lindström ◽  
...  
Keyword(s):  
Fuel Cells ◽  
Fuel Cell ◽  
Surface Charge ◽  
Proton Exchange Membrane ◽  
Membrane Surface ◽  
Proton Exchange ◽  
Membrane Thickness ◽  
Fuel Cell Performance ◽  
Cellulose Nanofibres ◽  
Exchange Membrane

The performance of thin carboxylated cellulose nanofiber-based (CNF) membranes as proton exchange membranes in fuel cells has been measured in-situ as a function of CNF surface charge density (600 and 1550 µmol g<sup>-1</sup>), counterion (H<sup>+</sup>or Na<sup>+</sup>), membrane thickness and fuel cell relative humidity (RH 55 to 95 %). The structural evolution of the membranes as a function of RH as measured by Small Angle X-ray scattering shows that water channels are formed only above 75 % RH. The amount of absorbed water was shown to depend on the membrane surface charge and counter ions (Na<sup>+</sup>or H<sup>+</sup>). The high affinity of CNF for water and the high aspect ratio of the nanofibers, together with a well-defined and homogenous membrane structure, ensures a proton conductivity exceeding 1 mS cm<sup>-1</sup>at 30 °C between 65 and 95 % RH. This is two orders of magnitude larger than previously reported values for cellulose materials and only one order of magnitude lower than Nafion 212. Moreover, the CNF membranes are characterized by a lower hydrogen crossover than Nafion, despite being ≈ 30 % thinner. Thanks to their environmental compatibility and promising fuel cell performance the CNF membranes should be considered for new generation proton exchange membrane fuel cells.<br>

Kerusakan Pada Material Pipa Air Bersih Akibat General Corrosion

2019 ◽  
Vol 16 (2) ◽  
Author(s):  
M. N. Setia Nusa
Keyword(s):  
Chemical Composition ◽  
Failure Analysis ◽  
External Surface ◽  
Internal Surface ◽  
General Corrosion ◽  
Water Pipe ◽  
Corrosion Attack ◽  
Corrosion Failure ◽  
Environment Factor

Water pipe of 4.5” diameter and has been operated for 8 year having failure due to corrosion attack on its external and internal surface. It is conducted failure analysis to find out the cause of corrosion by having testing examination of visual fractography, metalography,, SEM EDAX, hardness and chemical composition. Testing and examination results show that the failure / corroded pipe has a for in of general corrosion on the external surface due to environment factor or ground factor arround the pipe and on the internal surface was influenced by excessive root weld creating protrude which then causing turbolens and deposite to accelerate corrosion attack.Pipa berdiameter 4.5” yang berfungsi mengalirkan air bersih dan telah beroperasi selama 8 tahun, terjadi kerusakan berbentuk korosi pada permukaan luar pipa dan permukaan dalam. Untuk itu dilakukan analisa kerusakan untuk mengetahui penyebab terjadinya korosi dengan pengujian dan pemeriksaan secara visual,Fractography, Metallography, SEM, EDAX, Uji Kekerasan dan Uji Komposisi Kimia. Hasil pemeriksaan dan pengujian pada pipa yang rusak / korosi berbentuk jenis general korosi pada permukaan luar pipa yang diakibatkan faktor lingkungan atau tanah disekitar pipa, sedangkan pada bagian dalam pipa dipengaruhi oleh adanya lelehan pengelasan yang kurang sempurna sehingga menimbulkan benjolan yang mengakibatkan aliran air didalam pipa tidak lancar sehingga terjadi turbolensi yang menimbulkan endapan dan mengakibatkan percepatan terjadinya korosi.Keywords: Pipe, environtment, turbolens, corrosion, failure

scholarly journals Experimental Determination of the Heat Transfer Coefficient of Real Cooled Geometry Using Linear Regression Method

Energies ◽  
2020 ◽  
Vol 14 (1) ◽  
pp. 180
Author(s):  
Asif Ali ◽  
Lorenzo Cocchi ◽  
Alessio Picchi ◽  
Bruno Facchini
Keyword(s):  
Heat Transfer ◽  
Heat Flux ◽  
Heat Transfer Coefficient ◽  
Linear Regression ◽  
Surface Temperature ◽  
Transfer Coefficient ◽  
External Surface ◽  
Internal Surface ◽  
Regression Method ◽  
Thermal Transient

The scope of this work was to develop a technique based on the regression method and apply it on a real cooled geometry for measuring its internal heat transfer distribution. The proposed methodology is based upon an already available literature approach. For implementation of the methodology, the geometry is initially heated to a known steady temperature, followed by thermal transient, induced by injection of ambient air to its internal cooling system. During the thermal transient, external surface temperature of the geometry is recorded with the help of infrared camera. Then, a numerical procedure based upon a series of transient finite element analyses of the geometry is applied by using the obtained experimental data. The total test duration is divided into time steps, during which the heat flux on the internal surface is iteratively updated to target the measured external surface temperature. The final procured heat flux and internal surface temperature data of each time step is used to find the convective heat transfer coefficient via linear regression. This methodology is successfully implemented on three geometries: a circular duct, a blade with U-bend internal channel, and a cooled high pressure vane of real engine, with the help of a test rig developed at the University of Florence, Italy. The results are compared with the ones retrieved with similar approach available in the open literature, and the pros and cons of both methodologies are discussed in detail for each geometry.

scholarly journals Effect of Selected Anionic and Cationic Drugs Affecting the Central Nervous System on Electrical Properties of Phosphatidylcholine Liposomes: Experiment and Theory

2021 ◽  
Vol 22 (5) ◽  
pp. 2270
Author(s):  
Joanna Kotyńska ◽  
Monika Naumowicz
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Charge Density ◽  
Surface Charge ◽  
Surface Charge Density ◽  
Barbituric Acid ◽  
Membrane Surface ◽  
Adsorption Equilibria ◽  
Two Component ◽  
The Central Nervous System

Interactions between phospholipid membranes and selected drugs affecting the central nervous system (CNS) were investigated. Small, unilamellar liposomes were used as biomimetic cell membrane models. Microelectrophoretic experiments on two-component liposomes were performed using the electrophoretic light scattering technique (ELS). The effect of both positively (perphenazine, PF) and negatively (barbituric acid, BA) charged drugs on zwitterionic L-α-phosphatidylcholine (PC) membranes were analyzed. Experimental membrane surface charge density (d) data were determined as a function of pH. Quantitative descriptions of the adsorption equilibria formed due to the binding of solution ions to analyzed two-component membranes are presented. Binding constants of the solution ions with perphenazine and barbituric acid-modified membranes were determined. The results of our research show that both charged drugs change surface charge density values of phosphatidylcholine membranes. It can be concluded that perphenazine and barbituric acid are located near the membrane surface, interacting electrostatically with phosphatidylcholine polar heads.

Impact of ion exchange membrane surface charge and hydrophobicity on electroconvection at underlimiting and overlimiting currents

2017 ◽  
Vol 523 ◽  
pp. 36-44 ◽  
Author(s):  
K.A. Nebavskaya ◽  
V.V. Sarapulova ◽  
K.G. Sabbatovskiy ◽  
V.D. Sobolev ◽  
N.D. Pismenskaya ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Surface Charge ◽  
Membrane Surface ◽  
Ion Exchange Membrane ◽  
Exchange Membrane ◽  
Membrane Surface Charge
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