scholarly journals Contribution of the Excluded Volume of Bovine Serum Albumin for Solute Molecules to the Apparent Nonsolvent Water

1970 ◽  
Vol 56 (2) ◽  
pp. 180-190 ◽  
Author(s):  
G. W. F. H. Borst-Pauwels ◽  
D. A. Goldstein
Keyword(s):  
Bovine Serum Albumin ◽  
Vapor Pressure ◽  
Solute Concentration ◽  
Critical Examination ◽  
Excluded Volume ◽  
Apparent Increase ◽  
Bovine Albumin ◽  
Pressure Method ◽  
Order Of Magnitude

Addition of a macromolecule to a solution will give rise to a large excluded volume for the centers of the solute molecules. This will cause an apparent increase in solute concentration which is of the same order of magnitude as that associated with the nonsolvent volumes reported in the literature. A critical examination of one of the procedures used for the determination of nonsolvent water—the vapor pressure method of Hill—is given, and it is concluded that, with the use of this method, it is impossible to detect any significant nonsolvent water surrounding bovine albumin for either sugars or polyols. Generally, data reported in the literature for the nonsolvent water of proteins or other macromolecules will be too high unless they are corrected for the excluded volume.

A Critical Examination of Sachs’ Material-Removal Method for Determination of Residual Stress

2003 ◽  
Author(s):  
Anthony P. Parker
Keyword(s):  
Residual Stress ◽  
Residual Stresses ◽  
Critical Examination ◽  
Fatigue Lifetime ◽  
Experimental Procedure ◽  
Linear Elastic ◽  
Order Of Magnitude ◽  
Hoop Stresses ◽  
Elastic Unloading

Sachs’ method is an experimental procedure used primarily in the determination of residual stresses in autofrettaged thick cylinders. In its usual form it involves fixing axial and hoop direction strain gauges to the OD of a tube; strain readings are then obtained after each incremental removal of material from the bore. Sachs’ analysis assumes that the remaining tube unloads in linear-elastic fashion throughout the process and that superposition may therefore be employed to quantify the residual stresses within the original tube. By numerical simulation of two complete Sachs’ experimental sequences with ‘open end’ conditions it is demonstrated that the assumption of elastic unloading is invalidated by the Bauschinger effect. Sachs’ method thereby overestimates compressive residual bore hoop stresses in a typical tube by between 24% and 43%. If used as the basis for cyclic pressurization fatigue lifetime predictions with pre-existing cracks, such discrepancies will cause overestimates in fatigue lifetime of an order of magnitude. Sachs’ experimental procedure is therefore not recommended as a reliable or conservative method for determination of residual stress.

A Critical Examination of Sachs’ Material-Removal Method for Determination of Residual Stress

2004 ◽  
Vol 126 (2) ◽  
pp. 234-236 ◽  
Author(s):  
Anthony P. Parker
Keyword(s):  
Residual Stress ◽  
Residual Stresses ◽  
Critical Examination ◽  
Fatigue Lifetime ◽  
Experimental Procedure ◽  
Linear Elastic ◽  
Order Of Magnitude ◽  
Hoop Stresses ◽  
Elastic Unloading

Sachs’ method is an experimental procedure used primarily in the determination of residual stresses in autofrettaged thick cylinders. In its usual form it involves fixing axial and hoop direction strain gauges to the OD of a tube; strain readings are then obtained after each incremental removal of material from the bore. Sachs’ analysis assumes that the remaining tube unloads in linear-elastic fashion throughout the process and that superposition may therefore be employed to quantify the residual stresses within the original tube. By numerical simulation of two complete Sachs’ experimental sequences with “open end” conditions it is demonstrated that the assumption of elastic unloading is invalidated by the Bauschinger effect. Sachs’ method thereby overestimates compressive residual bore hoop stresses in a typical tube by between 24% and 43%. If used as the basis for cyclic pressurization fatigue lifetime predictions with pre-existing cracks, such discrepancies will cause overestimates in fatigue lifetime of an order of magnitude. Sachs’ experimental procedure is therefore not recommended as a reliable or conservative method for determination of residual stress.

Development of a method for multielemental determination in water by EDXRF with radioisotopic source of 238Pu.

2019 ◽  
Vol 7 (2A) ◽  
Author(s):  
Camilo Fuentes Serrano ◽  
Juan Reinaldo Estevez Alvares ◽  
Alfredo Montero Alvarez ◽  
Ivan Pupo Gonzales ◽  
Zahily Herrero Fernandez ◽  
...  
Keyword(s):  
Thin Layer ◽  
Expanded Uncertainty ◽  
Considerable Decrease ◽  
X Ray ◽  
Precipitation Efficiency ◽  
Method Of Determination ◽  
Sensitivity Curves ◽  
Order Of Magnitude ◽  
Metrological Parameters

A method for determination of Cr, Fe, Co, Ni, Cu, Zn, Hg and Pb in waters by Energy Dispersive X Ray Fluorescence (EDXRF) was implemented, using a radioisotopic source of 238Pu. For previous concentration was employed a procedure including a coprecipitation step with ammonium pyrrolidinedithiocarbamate (APDC) as quelant agent, the separation of the phases by filtration, the measurement of filter by EDXRF and quantification by a thin layer absolute method. Sensitivity curves for K and L lines were obtained respectively. The sensitivity for most elements was greater by an order of magnitude in the case of measurement with a source of 238Pu instead of 109Cd, which means a considerable decrease in measurement times. The influence of the concentration in the precipitation efficiency was evaluated for each element. In all cases the recoveries are close to 100%, for this reason it can be affirmed that the method of determination of the studied elements is quantitative. Metrological parameters of the method such as trueness, precision, detection limit and uncertainty were calculated. A procedure to calculate the uncertainty of the method was elaborated; the most significant source of uncertainty for the thin layer EDXRF method is associated with the determination of instrumental sensitivities. The error associated with the determination, expressed as expanded uncertainty (in %), varied from 15.4% for low element concentrations (2.5-5 μg/L) to 5.4% for the higher concentration range (20-25 μg/L).

scholarly journals Fluorescent Orthopalladated Complexes of 4-Aryliden-5(4H)-Oxazolones from the Kaede Protein: Synthesis and Characterization

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1238
Author(s):  
Eduardo Laga ◽  
David Dalmau ◽  
Sofía Arregui ◽  
Olga Crespo ◽  
Ana I. Jimenez ◽  
...  
Keyword(s):  
Intramolecular Interactions ◽  
Ortho Position ◽  
Fluorescent Properties ◽  
Ancillary Ligands ◽  
X Ray ◽  
Mononuclear Complexes ◽  
Fluorescence Measurements ◽  
Anionic Ligands ◽  
Order Of Magnitude

The goal of the work reported here was to amplify the fluorescent properties of 4-aryliden-5(4H)-oxazolones by suppression of the hula-twist non-radiative deactivation pathway. This aim was achieved by simultaneous bonding of a Pd center to the N atom of the heterocycle and the ortho carbon of the arylidene ring. Two different 4-((Z)-arylidene)-2-((E)-styryl)-5(4H)-oxazolones, the structures of which are closely related to the chromophore of the Kaede protein and substituted at the 2- and 4-positions of the arylidene ring (1a OMe; 1b F), were used as starting materials. Oxazolones 1a and 1b were reacted with Pd(OAc)2 to give the corresponding dinuclear orthometalated palladium derivates 2a and 2b by regioselective C–H activation of the ortho-position of the arylidene ring. Reaction of 2a (2b) with LiCl promoted the metathesis of the bridging carboxylate by chloride ligands to afford dinuclear 3a (3b). Mononuclear complexes containing the orthopalladated oxazolone and a variety of ancillary ligands (acetylacetonate (4a, 4b), hydroxyquinolinate (5a), aminoquinoline (6a), bipyridine (7a), phenanthroline (8a)) were prepared from 3a or 3b through metathesis of anionic ligands or substitution of neutral weakly bonded ligands. All species were fully characterized and the X-ray determination of the molecular structure of 7a was carried out. This structure has strongly distorted ligands due to intramolecular interactions. Fluorescence measurements showed an increase in the quantum yield (QY) by up to one order of magnitude on comparing the free oxazolone (QY < 1%) with the palladated oxazolone (QY = 12% for 6a). This fact shows that the coordination of the oxazolone to the palladium efficiently suppresses the hula-twist deactivation pathway.

scholarly journals Ice Coverage Induced by Depositing a Water Drop onto the Supercooled Substrate at Extreme Low Vapor Pressure

Crystals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 691
Author(s):  
Yugang Zhao ◽  
Zichao Zuo ◽  
Haibo Tang ◽  
Xin Zhang
Keyword(s):  
Vapor Pressure ◽  
Substrate Temperature ◽  
Water Drop ◽  
Industrial Processes ◽  
Aircraft Icing ◽  
Ice Coverage ◽  
Fundamental Understanding ◽  
Order Of Magnitude ◽  
The Impact ◽  
Kinetics Of

Icing/snowing/frosting is ubiquitous in nature and industrial processes, and the accretion of ice mostly leads to catastrophic consequences. The existing understanding of icing is still limited, particularly for aircraft icing, where direct observation of the freezing dynamics is inaccessible. In this work, we investigate experimentally the impact and freezing of a water drop onto the supercooled substrate at extremely low vapor pressure, to mimic an aircraft passing through clouds at a relatively high altitude, engendering icing upon collisions with pendant drops. Special attention is focused on the ice coverage induced by an impinging drop, from the perimeter pointing outward along the radial direction. We observed two freezing regimes: (I) spread-recoil-freeze at the substrate temperature of Ts = −15.4 ± 0.2 °C and (II) spread (incomplete)-freeze at the substrate temperature of Ts = −22.1 ± 0.2 °C. The ice coverage is approximately one order of magnitude larger than the frozen drop itself, and counterintuitively, larger supercooling yields smaller ice coverage in the range of interest. We attribute the variation of ice coverage to the kinetics of vapor diffusion in the two regimes. This fundamental understanding benefits the design of new anti-icing technologies for aircraft.

Expected Improvement in the Determination of G Using an X Pendulum

1997 ◽  
Vol 12 (11) ◽  
pp. 1967-1974 ◽  
Author(s):  
Kazuaki Kuroda ◽  
Mark A. Barton ◽  
Atsushi Onae ◽  
Yukinobu Miki
Keyword(s):  
Gravitational Constant ◽  
New Technique ◽  
Expected Improvement ◽  
Order Of Magnitude ◽  
Experimental Errors ◽  
A New Technique ◽  
Newtonian Gravitational Constant

We propose the application of a new technique, the X pendulum, to determine the Newtonian gravitational constant G. We evaluate the likely experimental errors for configurations realizable with existing technologies and show that improvement of the accuracy by an order of magnitude or more is possible.

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