scholarly journals STUDIES BASED ON A MALIGNANT TUMOR OF THE RABBIT

1924 ◽  
Vol 40 (5) ◽  
pp. 583-602 ◽  
Author(s):  
Wade H. Brown ◽  
Louise Pearce ◽  
Chester M. Van Allen
Keyword(s):  
Tumor Cells ◽  
Malignant Tumor ◽  
Death Rate ◽  
Experimental Conditions ◽  
Percentage Distribution ◽  
Paradoxical Situation ◽  
Secondary Tumors ◽  
Apparent Reduction ◽  
The Subject

A report is given of the results obtained by intratesticular inoculation of a malignant tumor of the rabbit based on a study of the first twenty generations. The subject is presented from the standpoint of variations in growth and malignancy as they occurred with continued transplantation. Essential features of the experimental conditions and of clinical and postmortem observations are condensed and recorded graphically. On analyzing the results of these experiments, it was found that many changes had occurred in the behavior of the tumor during transplantation, but that the changes were of a very irregular character and as a rule did not proceed constantly in any given direction. Moreover, the evidence as to the effect of transplantation on the growth and malignancy of the tumor was contradictory in that there was a great deal of evidence to show that there had been a decided increase in the activity and capacity for growth on the part of the tumor cells. But, with the exception of a few generations, there was an apparent reduction in the incidence and percentage distribution of secondary tumors, while the death rate was unaltered or actually diminished. It was believed that this paradoxical situation afforded a basis for an explanation of the results that had been obtained by transplantation.

scholarly journals Reversibility of acid and alkaline deoxyribonuclease deficiency in malignant tumor cells.

1981 ◽  
Vol 29 (9) ◽  
pp. 1053-1060 ◽  
Author(s):  
H S Taper
Keyword(s):  
Tumor Cells ◽  
Malignant Tumor ◽  
Tumor Necrosis ◽  
Lead Nitrate ◽  
Dnase Activity ◽  
Masking Effect ◽  
Experimental Conditions ◽  
Early Stages ◽  
Acid Dnase

The nature of DNAse deficiency, which appears to be characteristic for malignant tumor cells, was investigated by the histochemical lead nitrate technique under various experimental conditions. Reappearance of distinct alkaline and acid DNAse activity was observed on the periphery of spontaneously occurring tumor necrosis, at early stages of the in vitro induced tumor necrosis, in necrotic tumor cells after in vivo irradiation and after in vitro treatment with different compounds. A membrane releaser did not reactivate DNAses in viable tumor cells, whereas the homogenate from tumor tissue inhibited DNAses in normal rat liver. These findings indicate that alkaline and acid DNAse deficiency in malignant tumor cells is a reversible phenomenon. This reversal of enzymatic activity has different histochemical and chronological patterns and specific reactivating factors for each DNAse. The masking effect of DNAse activity in malignant tumor cells is probably linked to natural enzyme inhibitors and its reversal to early stages of tumor necrosis.

CYTOLOGICAL AND IMMUNOCYTOCHEMICAL DIAGNOSIS OF TUMOR PLEURITIS

2017 ◽  
Vol 63 (6) ◽  
pp. 894-899
Author(s):  
Viktor Novik ◽  
A. Nefedova ◽  
Ye. Yakubo ◽  
O. Ivanov ◽  
Yekaterina Shalina ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Malignant Tumor ◽  
Cytological Study ◽  
False Negative ◽  
Unknown Primary ◽  
Cytological Examination ◽  
Immunocytochemical Studies ◽  
Negative Conclusion ◽  
Primary Localization

Cytological examination of smears from the sediment after centrifugation of pleural fluids was performed in 479 patients who underwent examination and treatment at our institution in 2014-2016. In 249 (52%) patients tumor cells were not detected in smears, in 230 (48%) observations a suspicion (28 observations) or a confident conclusion (202 observations) on the presence of malignant tumor cells in the exudates was cytologically expressed. In 38 cases immunocytochemical studies was additionally performed. In two observations a false-negative conclusion about the absence of tumor cells in smears was expressed. The sensitivity of the cytological study in the diagnosis of malignant pleuritis was 99.0%. Affirmative cytological conclusions on the presence of malignant pleuritis were given in 87.0% of observations, suspicious cytological responses - in 12.0% of cases. Immunocytochemical studies significantly expanded the possibilities of cytological research and were of great importance in the diagnosis of metastases of tumors of unknown primary localization.

scholarly journals Impairing flow-mediated endothelial remodeling reduces extravasation of tumor cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Gautier Follain ◽  
Naël Osmani ◽  
Valentin Gensbittel ◽  
Nandini Asokan ◽  
Annabel Larnicol ◽  
...  
Keyword(s):  
Blood Flow ◽  
Tumor Cells ◽  
Molecular Mechanisms ◽  
Metastatic Progression ◽  
Metastatic Dissemination ◽  
Secondary Tumors ◽  
Flow Profiles ◽  
Endothelial Response ◽  
Life Threatening

AbstractTumor progression and metastatic dissemination are driven by cell-intrinsic and biomechanical cues that favor the growth of life-threatening secondary tumors. We recently identified pro-metastatic vascular regions with blood flow profiles that are permissive for the arrest of circulating tumor cells. We have further established that such flow profiles also control endothelial remodeling, which favors extravasation of arrested CTCs. Yet, how shear forces control endothelial remodeling is unknown. In the present work, we aimed at dissecting the cellular and molecular mechanisms driving blood flow-dependent endothelial remodeling. Transcriptomic analysis of endothelial cells revealed that blood flow enhanced VEGFR signaling, among others. Using a combination of in vitro microfluidics and intravital imaging in zebrafish embryos, we now demonstrate that the early flow-driven endothelial response can be prevented upon specific inhibition of VEGFR tyrosine kinase and subsequent signaling. Inhibitory targeting of VEGFRs reduced endothelial remodeling and subsequent metastatic extravasation. These results confirm the importance of VEGFR-dependent endothelial remodeling as a driving force of CTC extravasation and metastatic dissemination. Furthermore, the present work suggests that therapies targeting endothelial remodeling might be a relevant clinical strategy in order to impede metastatic progression.

High-throughput concentration of rare malignant tumor cells from large-volume effusions by multistage inertial microfluidics

Lab on a Chip ◽  
2022 ◽  
Author(s):  
Nan Xiang ◽  
Zhonghua Ni
Keyword(s):  
Tumor Cells ◽  
High Throughput ◽  
Malignant Tumor ◽  
Large Volume ◽  
Concentration Factor ◽  
Pleural Effusions ◽  
Inertial Microfluidics ◽  
Low Concentration ◽  
Malignant Pleural Effusions ◽  
On Chip

On-chip concentration of rare malignant tumor cells (MTCs) in malignant pleural effusions (MPEs) with a large volume is challenging. Previous microfluidic concentrators suffer from a low concentration factor (CF) and...

scholarly journals VE-PTP participates in vasculogenic mimicry by preventing autophagic degradation of VE-cadherin

2019 ◽  
Author(s):  
Daniel Delgado-Bellido ◽  
Concepción Bueno-Galera ◽  
Angel Garcia-Diaz ◽  
F. Javier Oliver
Keyword(s):  
Endothelial Cells ◽  
Malignant Melanoma ◽  
Tumor Cells ◽  
Malignant Tumor ◽  
Vasculogenic Mimicry ◽  
P120 Catenin ◽  
Human Malignant Melanoma ◽  
Aberrant Phenotype ◽  
Autophagic Degradation ◽  
Vascular Expression

AbstractAberrant extra-vascular expression of VE-cadherin has been observed in metastasis associated with Vasculogenic Mimicry (VM); we have recently shown that in VM prone (VM+) tumor cells VE-cadherin is mainly in the form of pVE-cadherin in Y658 allowing an increased plasticity that potentiates VM development. As excessive VE-cadherin phosphorylation is regulated by the phosphatase VEPTP in endothelial cells in the current study we analysed its role in this aberrant phenotype in malignant tumor cells. We show that human malignant melanoma cells VM+, also express VE-PTP although at lower levels than endothelial cells. The complex VE-PTP/VE-Cadherin/p120-catenin act as a safeguard to prevent VE-cadherin degradation by autophagy. Indeed, silencing of VE-PTP results in complete degradation of VE-cadherin with the features of autophagy and increases the global p120 tyrosine phosphorylation status. In summary, we show that VE-PTP is involved in VM formation and disruption of VE-PTP/VE-Cadherin/p120 complex results in enhanced autophagy in aggressive VM+ cells.

scholarly journals Extraneural metastases in medulloblastoma

2011 ◽  
Vol 69 (2b) ◽  
pp. 328-331 ◽  
Author(s):  
V M F Muoio ◽  
S O Shinjo ◽  
H Matushita ◽  
S Rosemberg ◽  
M J Teixeira ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Tumor Cells ◽  
Malignant Tumor ◽  
Ventriculoperitoneal Shunt ◽  
Clinical Profile ◽  
Invasive Growth ◽  
Extraneural Metastases

Medulloblastoma is the most common childhood malignant tumor of central nervous system, but it may also occur in adults. It presents high invasive growth with spreading of tumor cells into the leptomeningeal space along the neuroaxis early in the course of the disease. Extraneural metastases are rare but frequently lethal, occurring only in 1 to 5% of patients, and are related, in the most of cases, to the presence of ventriculoperitoneal shunt. Here we characterize the clinical profile of five cases of medulloblastoma with systemic spreading of tumor cells, also comparing them to cases already described in the literature.

A study of the localization of transferrin receptor in malignant tumor cells

1995 ◽  
Vol 28 (3-4) ◽  
pp. 172-180 ◽  
Author(s):  
Yutaka Kimijima ◽  
Nobuyuki Tanaka ◽  
Yoshinobu Eishi ◽  
Takao Miyamoto ◽  
Kenichi Shionoya ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Malignant Tumor ◽  
Transferrin Receptor

Non-apoptotic cell death in malignant tumor cells and natural compounds

2018 ◽  
Vol 420 ◽  
pp. 210-227 ◽  
Author(s):  
Jing Ye ◽  
Ruonan Zhang ◽  
Fan Wu ◽  
Lijuan Zhai ◽  
Kaifeng Wang ◽  
...  
Keyword(s):  
Cell Death ◽  
Tumor Cells ◽  
Malignant Tumor ◽  
Apoptotic Cell ◽  
Natural Compounds ◽  
Apoptotic Cell Death
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