scholarly journals TECHNIQUE OF CULTIVATING HUMAN TISSUES IN VITRO

1916 ◽  
Vol 24 (4) ◽  
pp. 367-372 ◽  
Author(s):  
Robert A. Lambert
Keyword(s):  
Human Serum ◽  
Human Plasma ◽  
Culture Medium ◽  
Mechanical Injury ◽  
Human Tissues ◽  
Tissue Cultures ◽  
Active Growth ◽  
Homologous Serum

1. Unmodified human plasma is not a satisfactory culture medium for human tissues owing to the susceptibility of human fibrin to digestion by tissue ferments. The necessary framework is thus destroyed before the cells begin to migrate. The difficulty can be overcome by adding to human plasma or serum a small quantity of fowl or pigeon plasma, the fibrin of which is highly resistant to digestion. Human tissues have been propagated in this medium for several months through subcultures, and growth in vitro can probably be maintained indefinitely. 2. Human tissues show no greater sensitiveness to changes in temperature and mechanical injury associated with preparation of cultures than those of lower animals. They may be preserved in an ordinary ice box at 10–15°C. as long as 6 or 8 days. Tissues obtained at operation give best results, but pieces of organs removed at autopsy 1 to 4 hours after death sometimes show active growth. 3. The presence of normally existing iso-antibodies (agglutinins and hemolysins) in human serum is without influence on the growth of human tissues in vitro. In other words, autogenous serum has no advantage in tissue cultures over homologous serum.

Comparison of results obtained with human serum and a protein solution as a supplement for in vitro fertilization culture medium

1992 ◽  
Vol 58 (3) ◽  
pp. 637-639 ◽  
Author(s):  
Gerritdina J. Huisman ◽  
Nadia M. Lo-A-Njoe ◽  
Albert Th. Alberda ◽  
Robert A. Leerentveld ◽  
Arie Verhoeff ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Human Serum ◽  
Culture Medium ◽  
Protein Solution ◽  
Vitro Fertilization ◽  
Comparison Of Results

scholarly journals THE DEVELOPMENT OF LEISHMANIA DONOVANI IN VITRO AT 37°C

1953 ◽  
Vol 97 (2) ◽  
pp. 177-188 ◽  
Author(s):  
William Trager
Keyword(s):  
Human Serum ◽  
Human Erythrocyte ◽  
Small Proportion ◽  
Culture Medium ◽  
Leishmania Donovani ◽  
Cell Extract ◽  
Rabbit Serum ◽  
Red Cell

Suspensions of leishmanias from the spleen of hamsters infected with Leishmania donovani were placed in culture flasks and incubated at 37°C. In a medium of human erythrocyte extract and human serum there appeared within a day or two aflagellate forms resembling leishmanias but larger, as well as other aflagellate forms more nearly resembling rounded leptomonads. These intermediate forms multiplied during the first 4 days of culture. They then slowly died off, despite frequent renewal of the culture medium. Sometimes a small proportion of motile, typical leptomonads also appeared in such cultures. Leptomonads from cultures maintained at 28°C., when placed in the human red cell extract-human serum medium and incubated at 37°C., survived at least 4 days. For both types of effect, human serum could be replaced by normal hamster serum but not by rabbit serum. Nicotinamide, added to the human red cell extract-human serum medium at a concentration of 400 mg. per 100 ml., completely prevented the development of intermediate forms from leishmanias and brought about the rapid death of leptomonads at 37°C.

scholarly journals MULTIPLICATION OF TUBERCLE BACILLI WITHIN MONONUCLEAR PHAGOCYTES IN TISSUE CULTURES DERIVED FROM NORMAL ANIMALS AND ANIMALS VACCINATED WITH BCG

1953 ◽  
Vol 97 (2) ◽  
pp. 235-245 ◽  
Author(s):  
Emanuel Suter
Keyword(s):  
Tissue Culture ◽  
Guinea Pig ◽  
Guinea Pigs ◽  
Culture Medium ◽  
Normal Animal ◽  
Mononuclear Phagocytes ◽  
Tissue Cultures ◽  
Inhibition Of Growth ◽  
Intracellular Multiplication

When monocytes derived from normal guinea pigs or rabbits were infected with tubercle bacilli and cultivated in vitro, the bacilli multiplied abundantly within the cytoplasm of these cells. By contrast, intracellular multiplication of the bacilli was retarded or completely inhibited within the monocytes of rabbits or guinea pigs vaccinated with BCG. This inhibition of growth was observed with both virulent or attenuated strains of tubercle bacilli. Under the conditions used in the present study, the ability of monocytes to inhibit bacillary proliferation was the same whether serum from a normal animal or from vaccinated animals was used in the tissue culture medium. Moreover, the serum of vaccinated animals did not inhibit multiplication of tubercle bacilli within monocytes derived from a normal animal. The ability of guinea pig monocytes to interfere with intracellular bacillary proliferation was first perceptible 8 days after vaccination.

scholarly journals Regulation of in vitro somatic embryogenesis with emphasis on to the role of endogenous hormones

2001 ◽  
Vol 13 (2) ◽  
pp. 196-223 ◽  
Author(s):  
VÍCTOR M. JIMÉNEZ
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Growth ◽  
Growth Regulators ◽  
Culture Medium ◽  
Endogenous Hormones ◽  
Tissue Cultures ◽  
Factors Associated ◽  
General Aspects

Different aspects of the in vitro somatic embryogenesis regulation are reviewed in this paper.work. A description of g General aspects, such as terminology, uses, stages of development and factors associated with the somatic embryogenesis, are described. is carried out. Although a brief description ofn the effects of the addition of different plant growth regulators to the culture medium wasis given, the article is centereds itself on the effect that the endogenous hormone concentrations in the initial explants and in the tissue cultures derived from them could play oin the induction and expression of somatic embryogenesis. It is significant that few to emphasize the low amount of systematic studies have been conducted, in this subject, in which different species and hormone groups were compared in cultures with and without embryogenic capacity. Moreover, the lack of correlation between the results presented in different studies the distinct works indicates that the hormone content of the cultures is not the only factor involved.

scholarly journals In vitro plant regeneration system for date palm (Phoenix dactylifera L.): effect of chelated iron sources

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Ahmed Madi Waheed Al-Mayahi
Keyword(s):  
Biochemical Analysis ◽  
Culture Medium ◽  
Date Palm ◽  
Phoenix Dactylifera ◽  
Tissue Cultures ◽  
In Vitro Plant Regeneration ◽  
Regeneration System ◽  
Iron Chelate ◽  
Phoenix Dactylifera L

Abstract Background Iron chelate sources and their concentrations are important factors in in vitro propagation of date palm. This study’s objective was to investigate the effect of the iron chelated form on the growth and development of tissue cultures of Barhee cultivar. Results The addition of FeEDDHA to the culture medium was more effective than FeEDTA on callus growth, shoot regeneration, and the number of shoots per jar, where the best result (220.8mg callus, 86.67% and 17.2 shoots per jar, respectively) was obtained by using 93.5 mg L−1 FeEDDHA (5.6 mg L−1 Fe), compared with other treatments. The results also indicate that using 93.5 mg L−1 FeEDDHA (5.6 mg L−1 Fe) as a supplement can decrease antioxidant enzymes CAT and POD activity compared to the rest of the treatments. Medium equipped with 187.0 mg L−1 FeEDDHA (11.2 mg L−1Fe) had the highest rooting percentage and number of roots per shoot than other treatments. The biochemical analysis results showed that treatments with FeEDDHA of 280.5 mg L−1 (16.8 mg L−1 Fe) and 187.0 mg L−1 (11.2 mg L−1Fe) significantly increased the iron content. The results showed that shoot maximum chlorophyll and endogenous IAA level content were recorded in a medium supplemented with 187.0 mg L−1 FeEDDHA (11.2 mg L−1Fe) as Fe source. Conclusion FeEDDHA used in the present study was proven to be a promising iron chelate source in comparison with the FeEDTA sources.

scholarly journals Lymphocyte-mediated lysis of antibody coated human red cells in the presence of human serum

Blood ◽  
1979 ◽  
Vol 53 (6) ◽  
pp. 1197-1202
Author(s):  
RJ Kurlander ◽  
WF Rosse
Keyword(s):  
Human Serum ◽  
Culture Medium ◽  
Human Albumin ◽  
Peripheral Blood Lymphocytes ◽  
Red Cells ◽  
The Other ◽  
High Concentration ◽  
The Third ◽  
Human Red Cells

When peripheral blood lymphocytes and human red cells coated with IgG were incubated in vitro in culture medium, antibody-dependent lymphocyte-mediated lysis was observed. This lysis was markedly inhibited by the addition of purified monoclonal IgG1 (1000 microgram/ml) to the culture medium. In contrast, lysis by lymphocytes of sensitized red cells in the presence of undiluted human serum was equal to or greater than lysis in medium alone, even in the presence of IgG1 at 1000 microgram/ml, despite the high concentration of IgG in human serum (6000--19,000 microgram/ml). Serum heated to 56 degrees C for 30 min also restored lysis in the presence of IgG1. When serum was separated into three fractions by passage through a Sephadex G-200 column, the third fraction, which contained proteins with a molecular weight of less than 100,000 d (but neither of the other two fractions nor purified human albumin), restored lymphocyte-mediated lysis in the presence of IgG1.

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