scholarly journals Components of the Ligand for a Ni++ Reactive Human T Cell Clone

2003 ◽  
Vol 197 (5) ◽  
pp. 567-574 ◽  
Author(s):  
Linh Lu ◽  
Jörg Vollmer ◽  
Corinne Moulon ◽  
Hans Ulrich Weltzien ◽  
Philippa Marrack ◽  
...  
Keyword(s):  
T Cell ◽  
Antigen Processing ◽  
Cell Clone ◽  
Histocompatibility Complex ◽  
Human T Cell ◽  
T Cell Clone ◽  
Restriction Element ◽  
Series Of Experiments ◽  
Β Chain ◽  
Specific Peptide

The major histocompatibility complex (MHC) restriction element for a human Ni2+ reactive T cell, ANi-2.3, was identified as DR52c. A series of experiments established that the functional ligand for this T cell was a preformed complex of Ni2+ bound to the combination of DR52c and a specific peptide that was generated in human and mouse B cells, but not in fibroblasts nor other antigen processing–deficient cells. In addition, ANi-2.3 recognition of this complex was dependent on His81 of the MHC β chain, suggesting a role for this amino acid in Ni2+ binding to MHC. We propose a general model for Ni2+ recognition in which βHis81 and two amino acids from the NH2-terminal part of the MHC bound peptide coordinate Ni2+ which then interacts with some portion of the Vα CDR1 or CDR2 region.

Human T cell autoimmunity against myelin basic protein: CD4+ cells recognizing epitopes of the T cell receptor β chain from a myelin basic protein-specific T cell clone

1993 ◽  
Vol 23 (2) ◽  
pp. 530-536 ◽  
Author(s):  
Güher Saruhan-Direskeneli ◽  
Frank Weber ◽  
Edgar Meinl ◽  
Martin Pette ◽  
Gerhard Giegerich ◽  
...  
Keyword(s):  
T Cell ◽  
Myelin Basic Protein ◽  
T Cell Receptor ◽  
Basic Protein ◽  
Cell Clone ◽  
Cell Receptor ◽  
Cd4 Cells ◽  
Human T Cell ◽  
T Cell Clone ◽  
Β Chain

Specificity and T cell receptor β chain usage of a human collagen type II-reactive T cell clone derived from a healthy individual

1992 ◽  
Vol 22 (1) ◽  
pp. 51-56 ◽  
Author(s):  
Tan Yan ◽  
Harald Burkhardt ◽  
Thomas Ritter ◽  
Barbara Bröker ◽  
Karl Heinz Mann ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Collagen Type ◽  
Cell Clone ◽  
Cell Receptor ◽  
Type Ii ◽  
Collagen Type Ii ◽  
T Cell Clone ◽  
Human Collagen ◽  
Β Chain

scholarly journals Antigen recognition by a T cell clone outside the context of the major histocompatibility complex. A role for Mls in antigen presentation.

1984 ◽  
Vol 159 (1) ◽  
pp. 305-312 ◽  
Author(s):  
S J Waters ◽  
S D Waksal ◽  
G P Norton ◽  
C A Bona
Keyword(s):  
Major Histocompatibility Complex ◽  
T Cell ◽  
Cell Clone ◽  
Antigen Recognition ◽  
Major Histocompatibility ◽  
Spleen Cells ◽  
Differentiation Markers ◽  
Histocompatibility Complex ◽  
T Cell Clone

A T cell clone isolated from antigen-primed CB6/F1 mice was shown to proliferate to keyhole limpet hemocyanin (KLH) in the presence of irradiated syngeneic F1 spleen cells, as well as spleen cells from either parental strain (BALB/c and C57BL/6). The genetic restriction involved in this antigen-specific proliferation was mapped using BXD (C57BL/6 X DBA/2) recombinant inbred strains of mice to the Mls gene on chromosome one. To exclude the role of Ia antigens as the restricting determinants, monoclonal anti-Ia antibodies were used to block the in vitro proliferative response of this clone. Although anti-Iab and anti-Iad blocked the proliferation of this clone to KLH in the presence of irradiated spleen cells from either parent, this effect was shown to be dependent on Ia molecules passively absorbed by the T cell clone from the irradiated filler cells. Since the T clone expressed Thy-1.2 and Lyt-1+ differentiation markers, its helper activity was compared with other KLH carrier-specific clones in an in vitro antibody synthesis assay. The Mls-KLH-restricted T cell clone, in contrast to other carrier-specific, major histocompatibility complex (MHC)-restricted T cell clones, was unable to cooperate with trinitrophenyl (TNP)-primed B cells in the presence of TNP-KLH to generate an anti-TNP response. These experiments suggest that non-MHC determinants, such as autologous Mls gene products, may play a role in genetically restricted antigen recognition by T lymphocytes.

Antisuppressive activity of a human T cell clone expressing a π/δ-receptor

1988 ◽  
Vol 23 (2) ◽  
pp. 132 ◽  
Author(s):  
G. Pawelec ◽  
A. Rehbein ◽  
I. Balko ◽  
H.-J. Bühring
Keyword(s):  
T Cell ◽  
Cell Clone ◽  
Human T Cell ◽  
T Cell Clone

scholarly journals Clonotypic structures involved in antigen-specific human T cell function. Relationship to the T3 molecular complex.

1983 ◽  
Vol 157 (2) ◽  
pp. 705-719 ◽  
Author(s):  
S C Meuer ◽  
K A Fitzgerald ◽  
R E Hussey ◽  
J C Hodgdon ◽  
S F Schlossman ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
T Cell ◽  
Cell Surface ◽  
Cell Function ◽  
Cell Clone ◽  
Interleukin 2 ◽  
Cytotoxic T Cell ◽  
Surface Molecules ◽  
Human T Cell ◽  
T Cell Clone

Monoclonal antibodies were produced against a human cytotoxic T cell clone, CT8III (specificity: HLA-A3), with the view of defining clonally restricted (clonotypic) surface molecules involved in its antigen recognition function. Two individual antibodies, termed anti-Ti1A and anti-Ti1B, reacted exclusively with the CT8III clone when tested on a panel of 80 additional clones from the same donor, resting or activated T cells, B cells, macrophages, thymocytes, or other hematopoietic cells. More importantly, the two antibodies inhibited cell-mediated killing and antigen-specific proliferation of the CT8III clone but did not affect the functions of any other clone tested. This inhibition was not secondary to generalized abrogation of the CT8III clone's function, because interleukin 2 responsiveness was enhanced. To examine the relationship of the structures defined by anti-clonotypic antibodies with known T cell surface molecules, antibody-induced modulation studies and competitive binding assays were performed. The results indicated that the clonotypic structures were associated with, but distinct from, the 20,000-mol wt T3 molecule expressed on all mature T lymphocytes. Moreover, in contrast to anti-T3, anti-Ti1A and anti-Ti1B each immunoprecipitated two molecules of 49,000 and 43,000-mol wt from 131I-labeled CT8III cells under reducing conditions. The development of monoclonal antibodies to such polymorphic T cell surface structures should provide important probes to further define the surface receptor for antigen.

scholarly journals An Amiloride-Sensitive and Voltage-Dependent Na+ Channel in an HLA-DR-Restricted Human T Cell Clone

2000 ◽  
Vol 165 (1) ◽  
pp. 83-90 ◽  
Author(s):  
Zhong-Fang Lai ◽  
Yu-Zhen Chen ◽  
Yasuharu Nishimura ◽  
Katsuhide Nishi
Keyword(s):  
T Cell ◽  
Cell Clone ◽  
Na Channel ◽  
Human T Cell ◽  
Hla Dr ◽  
T Cell Clone ◽  
Voltage Dependent
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