scholarly journals T cell determinants of myelin basic protein include a unique encephalitogenic I-E-restricted epitope for Lewis rats.

1989 ◽  
Vol 170 (2) ◽  
pp. 355-367 ◽  
Author(s):  
H Offner ◽  
G A Hashim ◽  
B Celnik ◽  
A Galang ◽  
X B Li ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Guinea Pig ◽  
Autoimmune Diseases ◽  
Myelin Basic Protein ◽  
Basic Protein ◽  
Lewis Rats ◽  
Gene Combination ◽  
First Time ◽  
Restricted Epitope

The major encephalitogenic epitope for Lewis rats is the 72-89 sequence of guinea pig basic protein (GP-BP) or rat basic protein (Rt-BP). T cells responsive to this epitope are I-A restricted and preferentially express the V alpha 2:V beta 8 gene combination in their TCR. In this work, we describe for the first time the delayed appearance of T cells specific for additional discrete determinant of BP, the nonencephalitogenic 55-68 sequence of GP-BP restricted by I-A, and the encephalitogenic 87-99 sequence of Rt-BP restricted by I-E. The TCR V alpha 2:V beta 8 gene combination was expressed by both encephalitogenic GP-BP S72-89 and Rt-BP S87-99 T cell specificities but not by GP-BP 44-68-specific T cells. This is the first demonstration of I-E-restricted encephalitogenic T cells in Lewis rats and supports the conclusion that the I-E class II locus is involved in autoimmune diseases.

Lewis Rat T Cells Can Reutilize, Process, and Present Myelin Basic Protein to Antigen-Specific T Cell Lines

1994 ◽  
Vol 156 (1) ◽  
pp. 36-53 ◽  
Author(s):  
Joanne M. St. Louis ◽  
John M. Pasick ◽  
Colleen Stein ◽  
David Freeman ◽  
Bhagirath Singh ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Myelin Basic Protein ◽  
Cell Lines ◽  
Basic Protein ◽  
Lewis Rat ◽  
T Cell Lines

scholarly journals Encephalitogenic T cell clones specific for myelin basic protein. An unusual bias in antigen recognition.

1985 ◽  
Vol 162 (6) ◽  
pp. 2107-2124 ◽  
Author(s):  
S S Zamvil ◽  
P A Nelson ◽  
D J Mitchell ◽  
R L Knobler ◽  
R B Fritz ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Myelin Basic Protein ◽  
Basic Protein ◽  
Lymphocytic Infiltration ◽  
Class Ii ◽  
Demyelinating Diseases ◽  
Complex Molecules ◽  
T Cell Clones ◽  
Cell Clones

Class II-restricted T cell clones specific for myelin basic protein (MBP) have been generated from PL/J and (PL/J X SJL/J)F1 [((PLSJ)F1] mice following sensitization to rat MBP. Of 17 T cell clones generated from (PLSJ)F1 mice, 5 are I-Au(A alpha uA beta u) restricted, one is restricted to I-As(A alpha sA beta s), 10 are restricted to hybrid I-A(u X s)F1 (A alpha sA beta u) determinants, and one clone is restricted to hybrid I-E(u X s) (E alpha uE beta s) molecules. Thus, of 16 I-A-restricted T cell clones generated from (PLSJ)F1 mice, only one is I-As-restricted, reflecting a lack of priming to MBP in association with I-As. T cell clones restricted to I-Au and to I-E (E alpha u E beta s) molecules recognize mouse (self) MBP. Furthermore, only the five T cell clones restricted to I-Au molecules recognize a determinant in common with mouse (self) MBP within the encephalitogenic N-terminal peptide. Three such I-Au restricted T cell clones, derived independently, cause paralysis in 100% of (PL/J X SJL/J)F1 mice tested. Acute, chronic unremitting, and chronic relapsing paralysis are all induced following injection of these clones. Administration of greater numbers of cloned T cells causes acute and fatal experimental allergic encephalomyelitis, while administration of lower numbers of cloned T cells is associated with chronic unremitting and relapsing paralysis. Paralysis induced with T cell clones shares many clinical, immunologic, and histologic aspects with human demyelinating diseases such as multiple sclerosis. Histopathology reveals perivascular lymphocytic infiltration, demyelination, and remyelination. These studies demonstrate the utility of T cell clones for analyzing the association between class II major histocompatibility complex molecules and disease susceptibility.

scholarly journals Transformation of human T-cell clones by Herpesvirus saimiri: intact antigen recognition by autonomously growing myelin basic protein-specific T cells

1993 ◽  
Vol 90 (23) ◽  
pp. 11049-11053 ◽  
Author(s):  
F Weber ◽  
E Meinl ◽  
K Drexler ◽  
A Czlonkowska ◽  
S Huber ◽  
...  
Keyword(s):  
Signal Transduction ◽  
T Cells ◽  
T Cell ◽  
Myelin Basic Protein ◽  
Interferon Gamma ◽  
Basic Protein ◽  
Herpesvirus Saimiri ◽  
T Cell Clones ◽  
Human T Cell ◽  
Cell Clones

Herpesvirus saimiri has recently been shown to immortalize human T cells. It was unknown, however, whether Herpesvirus saimiri transformation affects T-cell receptor (TCR) expression and signal transduction. In the present study, we have transformed CD4+ human T-cell clones specific for human myelin basic protein. The transformed T cells were grown in interleukin 2 and divided in the absence of antigen and antigen-presenting cells. They retained the membrane phenotype of activated T cells and secreted the cytokines interferon gamma and lymphotoxin, but interleukin 4 was not detected. Further, the transformed T cells continued to express the original TCR as demonstrated by TCR variable-region-V beta-specific monoclonal antibodies and TCR sequencing. Antigen-specific recognition and signal transduction by the TCR were demonstrated by myelin-basic-protein-induced HLA-DR-restricted secretion of interferon gamma and lymphotoxin and by myelin-basic-protein-specific proliferation. Antigen specificity and reactivity have been maintained for > 1 year after transformation. Transformation with Herpesvirus saimiri now allows the production of virtually unlimited numbers of (auto)antigen-specific T cells expressing functional TCR and a stable membrane phenotype. This technology will facilitate studies of the pathogenesis of putative autoimmune diseases, such as multiple sclerosis, and may be of help in TCR-targeted immunotherapy.

scholarly journals Regulatory T Cells Maintain Long-Term Tolerance to Myelin Basic Protein by Inducing a Novel, Dynamic State of T Cell Tolerance

2007 ◽  
Vol 178 (2) ◽  
pp. 887-896 ◽  
Author(s):  
Sarah E. Cabbage ◽  
Eric S. Huseby ◽  
Blythe D. Sather ◽  
Thea Brabb ◽  
Denny Liggitt ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Regulatory T Cells ◽  
Myelin Basic Protein ◽  
Basic Protein ◽  
Dynamic State ◽  
T Cell Tolerance ◽  
Cell Tolerance

Lipoderivatives of an immunodominant epitope of myelin basic protein increased T cell responsiveness in Lewis rats

2005 ◽  
pp. 700-702
Author(s):  
Anna M. Papini ◽  
Silvia Mazzucco ◽  
Daniela Pinzani ◽  
Massimo Biondi ◽  
Mario Chelli ◽  
...  
Keyword(s):  
T Cell ◽  
Myelin Basic Protein ◽  
Basic Protein ◽  
Immunodominant Epitope ◽  
Lewis Rats
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