scholarly journals A unique V-J-C-rearranged gene encodes a gamma protein expressed on the majority of CD3+ T cell receptor-alpha/beta- circulating lymphocytes.

1988 ◽  
Vol 167 (2) ◽  
pp. 694-699 ◽  
Author(s):  
F Triebel ◽  
F Faure ◽  
M Graziani ◽  
S Jitsukawa ◽  
M P Lefranc ◽  
...  
Keyword(s):  
T Cell Receptor ◽  
Peripheral Blood ◽  
Antigenic Determinant ◽  
Cell Receptor ◽  
Gamma Chain ◽  
Gene Encoding ◽  
Circulating Lymphocytes ◽  
Alpha Beta ◽  
Human Pbls ◽  
Cell Receptor Alpha

We have recently described an mAb, anti-Ti gamma A, that recognizes an antigenic determinant carried by a TCR gamma chain. This antibody binds to approximately 3% of human PBLs and delineates a CD2+, CD3+, TCR-alpha/beta-, CD4-, CD8+/-, CD5+, NKH1-, and HLA class II- subset. The present study was designed to identify the gene encoding the Ti gamma A epitope. A first analysis was carried out on a previously characterized TCR gamma + fetal-cloned cell line termed F6C7. It was found that F6C7 cells have one gamma rearrangement on each chromosome: one joins V gamma 3 to J gamma 1, and the second joins V gamma 9 to J gamma P. Because only the latter allele appeared to be transcribed in the F6C7 lymphocytes, these data strongly suggested that anti-Ti gamma A mAb is specific for either a V gamma 9 or a V gamma 9-J gamma P-encoded peptide. To confirm this point, we studied an additional series of 13 randomly selected Ti gamma A+ cloned cells derived from peripheral blood of three distinct adult individuals. Each one of these lymphocytes was shown to both possess and transcribe a V gamma 9-J gamma P-C gamma 1-rearranged gene. It is therefore concluded that a predominant subpopulation of CD3+ TCR-alpha/beta- human circulating T lymphocytes (namely, the subset defined by anti-Ti gamma A mAb) surface expresses a gamma protein with a limited potential of variability from one cell to another.

scholarly journals A novel subset of human lymphocytes with a T cell receptor-gamma complex.

1987 ◽  
Vol 166 (4) ◽  
pp. 1192-1197 ◽  
Author(s):  
S Jitsukawa ◽  
F Faure ◽  
M Lipinski ◽  
F Triebel ◽  
T Hercend
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Human Lymphocytes ◽  
Cell Receptor ◽  
Surface Expression ◽  
Gamma Chain ◽  
A Cells ◽  
Wide Range ◽  
Circulating Lymphocytes ◽  
Alpha Beta

We have previously characterized a CD3+ T cell receptor (TCR) alpha/beta- human fetal cloned cell line, termed F6C7, which surface-expresses a CD3-associated gamma chain identified by anti-NKFi, an mAb with a restricted clonotypic reactivity. Here, we have produced an additional antibody, anti-Ti-gamma A, which recognizes a public epitope of the gamma molecule defined by anti-NKFi. Ti-gamma A is present on approximately 3% of circulating lymphocytes with a wide range (1-15%) among 30 healthy individuals tested. Two-color immunofluorescence experiments performed with anti-Ti-gamma A and BMA 031 mAb (a reagent specific for the TCR-alpha/beta receptor) showed that surface expression of Ti-alpha/beta and Ti-gamma A is mutually exclusive. Moreover, it was found that most Ti-gamma A+ cells are CD2+, CD3+, CD4-, CD5+, NKH1-, HLA class II-negative. In contrast, the expression of the CD8 molecule on these T lymphocytes appears to be variable from one individual to another. Finally, we found that Ti-gamma A+ cells represent a majority of peripheral lymphocytes that express CD3 proteins but not the TCR-alpha/beta heterodimer. The delineation of this unique lymphocyte subset should help further studies on the biology of cells with a CD3-associated gamma complex.

Rearrangement of the T-cell receptor alpha, beta and gamma chain genes in chronic lymphocytic leukemia

1990 ◽  
Vol 14 (2) ◽  
pp. 131-137 ◽  
Author(s):  
Andras Perl ◽  
John P. Di Vincenzo ◽  
Daniel H. Ryan ◽  
Peter Gergely ◽  
Agnes Szigeti ◽  
...  
Keyword(s):  
T Cell ◽  
Chronic Lymphocytic Leukemia ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Lymphocytic Leukemia ◽  
Gamma Chain ◽  
Receptor Alpha ◽  
Alpha Beta ◽  
Cell Receptor Alpha

Lymphoid development in mice congenitally lacking T cell receptor alpha beta-expressing cells

Science ◽  
1992 ◽  
Vol 256 (5062) ◽  
pp. 1448-1452 ◽  
Author(s):  
K. Philpott ◽  
J. Viney ◽  
G Kay ◽  
S Rastan ◽  
E. Gardiner ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Lymphoid Development ◽  
Receptor Alpha ◽  
Alpha Beta ◽  
Cell Receptor Alpha

scholarly journals Oligoclonal repertoire of the CD8 alpha alpha and the CD8 alpha beta TCR-alpha/beta murine intestinal intraepithelial T lymphocytes: evidence for the random emergence of T cells.

1994 ◽  
Vol 180 (4) ◽  
pp. 1345-1358 ◽  
Author(s):  
A Regnault ◽  
A Cumano ◽  
P Vassalli ◽  
D Guy-Grand ◽  
P Kourilsky
Keyword(s):  
T Cells ◽  
Small Intestine ◽  
Lymph Node ◽  
T Cell Receptor ◽  
Global Analysis ◽  
Cell Receptor ◽  
Intraepithelial Lymphocytes ◽  
Beta Chain ◽  
Alpha Beta ◽  
Cell Receptor Alpha

The epithelium of the small intestine in normal euthymic mice contains a large number of intraepithelial lymphocytes (IEL), some of which bear a T cell receptor alpha/beta (TCR-alpha/beta). About half of these TCR-alpha/beta IEL display the CD8 alpha alpha phenotype and the remaining have the CD8 alpha beta or the CD4 phenotypes. To examine whether TCR-alpha/beta IEL have a TCR-beta chain repertoire as diverse as that of TCR-alpha/beta lymph node lymphocytes (LNL), we used a recently described PCR technique that allows a global analysis of the TCR-beta chain repertoire. Within any given mouse, the repertoires expressed in both CD8 alpha alpha and CD8 alpha beta TCR-alpha/beta IEL populations are oligoclonal and nonoverlapping between the two subsets. The clones are largely conserved through the length of the small intestine of the same individual. However, genetically identical individuals raised under indistinguishable environmental conditions display distinct oligoclonal repertoires. Those findings indicate that few cells of CD8 alpha alpha or of the CD8 alpha beta phenotype are responsible for the repopulation of the intestinal epithelium.

scholarly journals The presumptive CDR3 regions of both T cell receptor alpha and beta chains determine T cell specificity for myoglobin peptides.

1990 ◽  
Vol 172 (1) ◽  
pp. 27-33 ◽  
Author(s):  
J S Danska ◽  
A M Livingstone ◽  
V Paragas ◽  
T Ishihara ◽  
C G Fathman
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Amino Acid Sequences ◽  
Antigenic Specificity ◽  
Antigenic Peptides ◽  
Surface Complexes ◽  
Receptor Alpha ◽  
Alpha Beta ◽  
Cell Receptor Alpha

The T cell receptor alpha/beta (TCR-alpha/beta) is encoded by variable (V), diversity (D), joining (J), and constant (C) segments assembled by recombination during thymocyte maturation to produce a heterodimer that imparts antigenic specificity to the T cell. Unlike immunoglobulins (Igs), which bind free antigen, the ligands of TCR-alpha/beta are cell surface complexes of intracellularly degraded antigens (i.e., peptides) bound to and presented by polymorphic products of the major histocompatibility complex (MHC). Therefore, antigen recognition by T cells is defined as MHC restricted. A model has been formulated based upon the similarity between TCR-alpha/beta V region and Ig Fab amino acid sequences, and the crystal structure of the MHC class I and Ig molecules. This model predicts that the complementarity determining regions (CDR) 1 and 2, composed of TCR V alpha and V beta segments, primarily contact residues of the MHC alpha helices, whereas V/J alpha and V/D/J beta junctional regions (the CDR3 equivalent) contact the peptide in the MHC binding groove. Because polymorphism in MHC proteins is limited relative to the enormous diversity of antigenic peptides, the TCR may have evolved to position the highly diverse junctional residues (CDR3), where they have maximal contact with antigen bound in the MHC peptide groove. Here, we demonstrate a definitive association between CDR3 sequences in both TCR alpha and beta chains, and differences in recognition of antigen fine specificity using a panel of I-Ed-restricted, myoglobin-reactive T cell clones. Acquisition of these data relied in part upon a modification of the polymerase chain reaction that uses a degenerate, consensus primer to amplify TCR alpha chains without foreknowledge of the V alpha segments they utilize.

scholarly journals Lymphocyte proliferation in mice congenitally deficient in T-cell receptor alpha beta + cells.

1994 ◽  
Vol 91 (25) ◽  
pp. 11948-11952 ◽  
Author(s):  
J. L. Viney ◽  
L. Dianda ◽  
S. J. Roberts ◽  
L. Wen ◽  
C. A. Mallick ◽  
...  
Keyword(s):  
T Cell ◽  
Beta Cells ◽  
T Cell Receptor ◽  
Lymphocyte Proliferation ◽  
Cell Receptor ◽  
Receptor Alpha ◽  
Alpha Beta ◽  
Cell Receptor Alpha

scholarly journals Regulation of pre-T cell receptor (pT alpha-TCR beta) gene expression during human thymic development.

1996 ◽  
Vol 184 (2) ◽  
pp. 519-530 ◽  
Author(s):  
A R Ramiro ◽  
C Trigueros ◽  
C Márquez ◽  
J L San Millán ◽  
M L Toribio
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
T Cell Development ◽  
Cell Receptor ◽  
Beta Chain ◽  
Thymocyte Development ◽  
Gene Encoding ◽  
Thymic Development ◽  
Alpha Beta ◽  
Beta Gene

In murine T cell development, early thymocytes that productively rearrange the T cell receptor (TCR) beta locus are selected to continue maturation, before TCR alpha expression, by means of a pre-TCR alpha- (pT alpha-) TCR beta heterodimer (pre-TCR). The aim of this study was to identify equivalent stages in human thymocyte development. We show here that variable-diversity-joining region TCR beta rearrangement and the expression of full-length TCR beta transcripts have been initiated in some immature thymocytes at the TCR alpha/beta- CD4+CD8- stage, and become common in a downstream subset of TCR alpha/beta- CD4+CD8+ thymocytes that is highly enriched in large cycling cells. TCR beta chain expression was hardly detected in TCR alpha/beta- CD4+CD8- thymocytes, whereas cytoplasmic TCR beta chain was found in virtually all TCR alpha/beta- CD4+CD8+ blasts. In addition, a TCR beta complex distinct from the mature TCR alpha/beta heterodimer was immunoprecipitated only from the latter subset. cDNA derived from TCR alpha/beta- CD4+CD8+ blasts allowed us to identify and clone the gene encoding the human pT alpha chain, and to examine its expression at different stages of thymocyte development. Our results show that high pT alpha transcription occurs only in CD4+CD8- and CD4+CD8+ TCR alpha/beta- thymocytes, whereas it is weaker in earlier and later stages of development. Based on these results, we propose that the transition from TCR alpha/beta- CD4+CD8- to TCR alpha/beta- CD4+CD8+ thymocytes represents a critical developmental stage at which the successful expression of TCR beta promotes the clonal expansion and further maturation of human thymocytes, independent of TCR alpha.

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