scholarly journals Correlation of T cell receptor V beta gene family with MHC restriction.

1987 ◽  
Vol 166 (2) ◽  
pp. 583-588 ◽  
Author(s):  
P A Morel ◽  
A M Livingstone ◽  
C G Fathman
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Sperm Whale ◽  
Cell Receptor ◽  
Chain Gene ◽  
Beta Chain ◽  
T Cell Clones ◽  
Mhc Restriction ◽  
Cell Clones ◽  
Beta Chain Genes

We have studied a panel of DBA/2 T cell clones specific for sperm whale myoglobin (SpW Mb) for TCR (T cell receptor) beta chain gene expression by FACS analysis using the monoclonal antibodies F23.1 and KJ16 specific for the V beta 8 family of the TCR beta chain genes. Within any given specificity group, all the clones tested came from different mice. 10 of 11 I-Ed-restricted SpW Mb-specific T cell clones were F23.1+; 8 of these were also KJ16+. Only one of the three I-Ad-restricted clones tested was F23.1+; this clone was KJ16 negative. This study has demonstrated that I-Ed-restricted T cell clones from DBA/2 mice express members of the TCR V beta 8 family irrespective of the epitopes of SpW Mb recognized. These data suggest an apparent correlation between TCR V beta expression and MHC restriction.

Preferential expression of a defined T-cell receptor β-chain gene in hapten-specific cytotoxic T-cell clones

Nature ◽  
1986 ◽  
Vol 322 (6077) ◽  
pp. 376-378 ◽  
Author(s):  
Ute Hochgeschwender ◽  
Hans Ulrich Weltzien ◽  
Klaus Eichmann ◽  
R. Bruce Wallace ◽  
Jörg T. Epplen
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Cytotoxic T Cell ◽  
Chain Gene ◽  
T Cell Clones ◽  
Preferential Expression ◽  
Cell Clones ◽  
Β Chain

Detection of Putative T cell Clones Using T cell Receptor β Chain Gene Clonality Assay in Korean Patients with Aplastic Anemia

2009 ◽  
Vol 29 (4) ◽  
pp. 269-276 ◽  
Author(s):  
Hyun-Jung Choi ◽  
Myung-Geun Shin ◽  
Hye-Ran Kim ◽  
Hyeoung-Joon Kim ◽  
Hoon Kook ◽  
...  
Keyword(s):  
T Cell ◽  
Aplastic Anemia ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Chain Gene ◽  
T Cell Clones ◽  
Korean Patients ◽  
Cell Clones ◽  
Β Chain

scholarly journals Predominant expression of a T cell receptor V beta gene subfamily in autoimmune encephalomyelitis.

1988 ◽  
Vol 167 (5) ◽  
pp. 1586-1596 ◽  
Author(s):  
S S Zamvil ◽  
D J Mitchell ◽  
N E Lee ◽  
A C Moore ◽  
M K Waldor ◽  
...  
Keyword(s):  
T Cell ◽  
Mhc Class Ii ◽  
Cell Receptor ◽  
T Cell Response ◽  
Chain Gene ◽  
Beta Chain ◽  
Autoimmune Encephalomyelitis ◽  
Cell Clones ◽  
Beta Chain Genes

TCR beta chain gene expression of individual T cell clones that share the same MHC class II restriction and similar fine specificity for the encephalitogenic NH2 terminus of the autoantigen myelin basic protein (MBP) has been examined. TCR V beta expression was examined by FACS analysis with mAbs specific for the V beta 8 subfamily of TCR beta chain genes. 14 of 18 (78%) NH2-terminal MBP-specific clones examined express a member of the TCR V beta 8 subfamily. Southern analysis was used to identify which member(s) of the TCR V beta 8 subfamily is expressed by these clones. Each of four clones examined uses V beta 8.2, though two different V beta 8.2-J beta 2 combinations were identified. Our findings indicate that there is restricted TCR V beta usage in the autoimmune T cell response to the dominant encephalitogenic NH2-terminal epitope of the MBP. The use of an mAb to the antigen-specific TCR in the prevention of T cell-mediated autoimmune disease has been investigated. Our results demonstrate that in vivo administration of a TCR V beta 8-specific mAb prevents induction of autoimmune encephalomyelitis.

Fine specificity and T-cell receptor ?-chain gene rearrangements of five H-2Db-specific cytotoxic T-cell clones

1987 ◽  
Vol 25 (3) ◽  
pp. 171-178 ◽  
Author(s):  
Marco W. Schilham ◽  
Rosmarie Lang ◽  
Hans Acha-Orbea ◽  
Robbert Benner ◽  
Rolf Joho ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Cytotoxic T Cell ◽  
Chain Gene ◽  
Gene Rearrangements ◽  
T Cell Clones ◽  
Fine Specificity ◽  
Cell Clones ◽  
Receptor Chain

scholarly journals Detection of T cell receptor alpha chain locus rearrangements in human antigen-specific T lymphocyte clones using a DNA probe specific for chromosome region 14q11.

1986 ◽  
Vol 163 (5) ◽  
pp. 1308-1313 ◽  
Author(s):  
D Mathieu-Mahul ◽  
L K Chen ◽  
C J Larsen ◽  
A Bensussan
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cdna Probe ◽  
Cell Receptor ◽  
Dna Probe ◽  
Chain Gene ◽  
T Cell Clones ◽  
Alpha Chain ◽  
Chain Locus ◽  
Cell Clones

Human cloned antigen-specific T lymphocytes were used to characterize DNA rearrangements using a cDNA probe from the T cell receptor (TCR)-alpha chain gene. Rearranged patterns were detected in some T cell clones, confirming that normal mature T cells are rearranged in TCR-alpha locus. Similarly, rearranged DNA patterns were found in T cell clones from the same panel, using a DNA probe (clone K-40, [1]) isolated from chromosome 14 (14q11), where the TCR-alpha locus has been mapped. These results suggest that this genomic DNA clone is located within the TCR-alpha chain locus.

The expansion and selection of T cell receptor αβ intestinal intraepithelial T cell clones

1996 ◽  
Vol 26 (4) ◽  
pp. 914-921 ◽  
Author(s):  
Armelle Regnault ◽  
Jean-Pierre Levraud ◽  
Annick Lim ◽  
Adrien Six ◽  
Christiane Moreau ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
T Cell Clones ◽  
Cell Clones ◽  
Selection Of

scholarly journals Diversification of the antigen-specific T cell receptor repertoire after varicella zoster vaccination

2016 ◽  
Vol 8 (332) ◽  
pp. 332ra46-332ra46 ◽  
Author(s):  
Qian Qi ◽  
Mary M. Cavanagh ◽  
Sabine Le Saux ◽  
Hong NamKoong ◽  
Chulwoo Kim ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Receptor ◽  
Chronic Infection ◽  
Cell Receptor ◽  
Control Mechanisms ◽  
Cell Repertoire ◽  
T Cell Clones ◽  
Varicella Zoster ◽  
Cell Clones

Diversity and size of the antigen-specific T cell receptor (TCR) repertoire are two critical determinants for successful control of chronic infection. Varicella zoster virus (VZV) that establishes latency during childhood can escape control mechanisms, in particular with increasing age. We examined the TCR diversity of VZV-reactive CD4 T cells in individuals older than 50 years by studying three identical twin pairs and three unrelated individuals before and after vaccination with live attenuated VZV. Although all individuals had a small number of dominant T cell clones, the breadth of the VZV-specific repertoire differed markedly. A genetic influence was seen for the sharing of individual TCR sequences from antigen-reactive cells but not for repertoire richness or the selection of dominant clones. VZV vaccination favored the expansion of infrequent VZV antigen–reactive TCRs, including those from naïve T cells with lesser boosting of dominant T cell clones. Thus, vaccination does not reinforce the in vivo selection that occurred during chronic infection but leads to a diversification of the VZV-reactive T cell repertoire. However, a single-booster immunization seems insufficient to establish new clonal dominance. Our results suggest that repertoire analysis of antigen-specific TCRs can be an important readout to assess whether a vaccination was able to generate memory cells in clonal sizes that are necessary for immune protection.

scholarly journals p70 lupus autoantigen binds the enhancer of the T-cell receptor beta-chain gene.

1993 ◽  
Vol 90 (7) ◽  
pp. 2685-2689 ◽  
Author(s):  
H. Messier ◽  
T. Fuller ◽  
S. Mangal ◽  
H. Brickner ◽  
S. Igarashi ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Receptor ◽  
Cell Receptor ◽  
Chain Gene ◽  
Beta Chain ◽  
T Cell Receptor Beta ◽  
Receptor Beta
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