scholarly journals A low polymorphic mouse H-2 class I gene from the Tla complex is expressed in a broad variety of cell types.

1987 ◽  
Vol 166 (2) ◽  
pp. 341-361 ◽  
Author(s):  
C Transy ◽  
S R Nash ◽  
B David-Watine ◽  
M Cochet ◽  
S W Hunt ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Cdna Clone ◽  
Cell Types ◽  
Class I ◽  
Surface Molecule ◽  
Coding Region ◽  
Genes Encoding ◽  
Broad Variety ◽  
I Gene

We have previously described the isolation of pH-2d-37, a cDNA clone that encodes a so far unknown, poorly polymorphic, class I surface molecule. We report here the isolation of the corresponding gene, its nucleotide sequence, and its localization in the Tla region of the murine MHC. Using a RNase mapping assay, we have confirmed that the second domain coding region of the 37 gene displays very limited polymorphism, and that the gene is transcribed in a broad variety of cell types, in contrast to the genes encoding the known Qa and TL antigens. Possible functions are discussed.

Nucleotide sequence of a rat class I cDNA clone

1989 ◽  
Vol 29 (2) ◽  
pp. 134-137 ◽  
Author(s):  
Andelka Radojcic ◽  
Kimberley S. Stranick ◽  
Joseph Locker ◽  
Heinz W. Kunz ◽  
Thomas J. Gill
Keyword(s):  
Nucleotide Sequence ◽  
Cdna Clone ◽  
Class I

Stable transfer and restricted expression of a cloned class I gene encoding a secreted transplantation-like antigen

1985 ◽  
Vol 5 (6) ◽  
pp. 1295-1300
Author(s):  
Y Barra ◽  
K Tanaka ◽  
K J Isselbacher ◽  
G Khoury ◽  
G Jay
Keyword(s):  
Molecular Mechanisms ◽  
Cell Types ◽  
Class I ◽  
Regulatory Sequences ◽  
Transcriptional Enhancer ◽  
Gene Encoding ◽  
Specific Expression ◽  
Tissue Specific ◽  
Functional Studies ◽  
I Gene

The identification of a unique major histocompatibility complex class I gene, designated Q10, which encodes a secreted rather than a cell surface antigen has led to questions regarding its potential role in regulating immunological functions. Since the Q10 gene is specifically activated only in the liver, we sought to define the molecular mechanisms which control its expression in a tissue-specific fashion. Results obtained by transfection of the cloned Q10 gene, either in the absence or presence of a heterologous transcriptional enhancer, into a variety of cell types of different tissue derivations are consistent with the Q10 gene being regulated at two levels. The first is by a cis-dependent mechanism which appears to involve site-specific DNA methylation. The second is by a trans-acting mechanism which would include the possibility of an enhancer binding factor. The ability to efficiently express the Q10 gene in certain transfected cell lines offers an opportunity to obtain this secreted class I antigen in quantities sufficient for functional studies; this should also make it possible to define regulatory sequences which may be responsible for the tissue-specific expression of Q10.

Molecular cloning and nucleotide sequence analysis of the putative cDNA for the precursor molecule of the chicken LH-β subunit

1989 ◽  
Vol 3 (2) ◽  
pp. 129-137 ◽  
Author(s):  
T. Noce ◽  
H. Ando ◽  
T. Ueda ◽  
K. Kubokawa ◽  
T. Higashinakagawa ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Cdna Clone ◽  
Rabbit Antiserum ◽  
Nucleotide Sequence Analysis ◽  
Cdna Expression Library ◽  
Β Subunit ◽  
Coding Region ◽  
Precursor Molecule ◽  
Cdna Insert ◽  
Hybridization Probe

ABSTRACT A cDNA expression library was constructed from poly(A)+ RNA of broiler chicken adenohypophyses using λ gt11 as a vector. After screening with a rabbit antiserum against chicken LH, a cDNA clone (L12) containing a 436 bp insert was obtained. Using a subclone of L12 in pUC19 (pL12) as the hybridization probe, another cDNA clone (LF127) with a 533 bp insert was isolated. The LF127 contained the full-length cDNA encoding the putative chicken LH-β subunit precursor molecule. Hybridization of the pL12 cDNA insert to adenohypophysial RNA showed that chicken and Japanese quail adenohypophyses contained RNA species of about 0·8 and 1·0 kb respectively. The amount of this RNA species was ten times higher in adult male quails kept under long days at room temperature than in those kept under short days at 7 °C. In-situ hybridization experiments showed the exclusive distribution of the signal in the LH cells of the adenohypophysis. The similarity of the nucleotide sequence of the apoprotein-coding region of LH-β cDNA of the chicken to that of mammals is lower than that among mammals. The deduced amino acid sequence of the chicken LH-β subunit supports the hypothesis that the number of proline residues increases in the LH-β subunit the closer phylogenetically the vertebrate is to mammals.

scholarly journals Stable transfer and restricted expression of a cloned class I gene encoding a secreted transplantation-like antigen.

1985 ◽  
Vol 5 (6) ◽  
pp. 1295-1300 ◽  
Author(s):  
Y Barra ◽  
K Tanaka ◽  
K J Isselbacher ◽  
G Khoury ◽  
G Jay
Keyword(s):  
Molecular Mechanisms ◽  
Cell Types ◽  
Class I ◽  
Regulatory Sequences ◽  
Transcriptional Enhancer ◽  
Gene Encoding ◽  
Specific Expression ◽  
Tissue Specific ◽  
Functional Studies ◽  
I Gene

The identification of a unique major histocompatibility complex class I gene, designated Q10, which encodes a secreted rather than a cell surface antigen has led to questions regarding its potential role in regulating immunological functions. Since the Q10 gene is specifically activated only in the liver, we sought to define the molecular mechanisms which control its expression in a tissue-specific fashion. Results obtained by transfection of the cloned Q10 gene, either in the absence or presence of a heterologous transcriptional enhancer, into a variety of cell types of different tissue derivations are consistent with the Q10 gene being regulated at two levels. The first is by a cis-dependent mechanism which appears to involve site-specific DNA methylation. The second is by a trans-acting mechanism which would include the possibility of an enhancer binding factor. The ability to efficiently express the Q10 gene in certain transfected cell lines offers an opportunity to obtain this secreted class I antigen in quantities sufficient for functional studies; this should also make it possible to define regulatory sequences which may be responsible for the tissue-specific expression of Q10.

scholarly journals Organization, transcription and regulation of the Leishmania infantum histone H3 genes

1996 ◽  
Vol 318 (3) ◽  
pp. 813-819 ◽  
Author(s):  
Manuel SOTO ◽  
Jose M REQUENA ◽  
Luis QUIJADA ◽  
Carlos ALONSO
Keyword(s):  
Nucleotide Sequence ◽  
Leishmania Infantum ◽  
Histone H3 ◽  
State Level ◽  
Protozoan Parasite ◽  
Control Mechanisms ◽  
Mrna Levels ◽  
Coding Region ◽  
Genes Encoding ◽  
Multiple Copies

The genomic organization and transcription of the genes encoding the histone H3 of the protozoan parasite Leishmania infantum have been studied. It was found that there are multiple copies of the histone H3 genes distributed in chromosomal bands XIX and XIV. The nucleotide sequence of two of the L. infantum H3 genes, each one located in a different chromosome, is reported. Although the nucleotide sequence of the coding region of both genes is identical, the sequence of the 3´ untranslated region is highly divergent. It was found also that there exist two different size classes of histone H3 transcripts, each one derived from a different gene, and that they are polyadenylated. The steady-state level of the transcripts dramatically decreases when the parasites enter the stationary phase of growth, suggesting a mode of regulation which is linked to the proliferation status of the cell. Unlike the replication-dependent histones, the L. infantum H3 mRNA levels do not decrease after treatment with DNA synthesis inhibitors. A comparative analysis of the sensitivity of the histone mRNA levels to DNA inhibition in the parasites L. infantum and Trypanosoma cruzi revealed the existence of different control mechanisms in histone expression in these two phylogenetically related protozoan parasites.

The nucleotide sequence of a cDNA clone containing the entire coding region for mouse X-chromo- some-linked phosphoglycerate kinase

Gene ◽  
1986 ◽  
Vol 45 (3) ◽  
pp. 275-280 ◽  
Author(s):  
Mori Nozomu ◽  
Judith Singer-Sam ◽  
Lee Chi-Yu ◽  
Arthur D. Riggs
Keyword(s):  
Nucleotide Sequence ◽  
Cdna Clone ◽  
Phosphoglycerate Kinase ◽  
Coding Region

Nucleotide sequence of the HLA-A26 class I gene: Identification of specific residues and molecular mapping of public HLA class I epitopes

1990 ◽  
Vol 27 (3) ◽  
pp. 155-166 ◽  
Author(s):  
Hélène Zinszner ◽  
Michel Masset ◽  
Jean-François Bourge ◽  
Jacques Colombani ◽  
Daniel Cohen ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Molecular Mapping ◽  
Hla Class I ◽  
Class I ◽  
Gene Identification ◽  
I Gene

Nucleotide sequence of a dog class I cDNA clone

1990 ◽  
Vol 31 (5-6) ◽  
pp. 400-404 ◽  
Author(s):  
Ulla M. Sarmiento ◽  
Rainer Storb
Keyword(s):  
Nucleotide Sequence ◽  
Cdna Clone ◽  
Class I

scholarly journals Expression and T cell recognition of hybrid antigens with amino-terminal domains encoded by Qa-2 region of major histocompatibility complex and carboxyl termini of transplantation antigens.

1985 ◽  
Vol 161 (5) ◽  
pp. 935-952 ◽  
Author(s):  
I Stroynowski ◽  
J Forman ◽  
R S Goodenow ◽  
S G Schiffer ◽  
M McMillan ◽  
...  
Keyword(s):  
T Lymphocyte ◽  
Cytotoxic T Lymphocyte ◽  
Class I ◽  
Coding Region ◽  
Amino Terminal ◽  
L Cells ◽  
Hybrid Class ◽  
Beta 2 Microglobulin ◽  
Transplantation Antigens ◽  
I Gene

Coding potential of the Q6 gene from the Qa-2a region of BALB/c Crgl mice was analyzed by a combination of hybrid class I gene construction and DNA-mediated gene transfer. Recombinant genes were created by exon shuffling of the 5' coding region of the Q6 gene and the 3' coding region of a gene encoding a transplantation antigen (Kd, Dd, or Ld), or the inverse. Some of these hybrid class I genes were expressed in the transfected mouse fibroblasts (L cells). The hybrid class I molecules encoded by the 5' end of the Q6 gene and the 3' end of the Ld gene precipitated as 45,000 mol wt molecules associated with beta 2-microglobulin. The expression of the hybrid proteins indicates that 926 basepairs of the 5' flanking region upstream of the structural Q6 gene contain a promoter that functions as a transcription initiation site in L cells. The 3' portion of the Q6 gene appears to be responsible for the lack of cell surface expression of the intact Q6 and the hybrid Ld/Q6 genes in mouse fibroblasts. Accordingly, this portion of the Q6 class I gene may play a regulatory role in tissue-specific expression. Serological analyses of hybrid Q6 proteins suggested that Q6 may be a structural gene for CR (H-2 crossreactive) antigen found normally on subpopulations of lymphocytes. If this identification is correct, Q6 gene will define a new category of class I genes encoding approximately 40,000 mol wt molecules and carrying a characteristic truncated cytoplasmic tail. Analysis of L cells transfected with Q6 hybrid genes demonstrated also that the cytotoxic T cells specific for Qa-2a region-coded antigens recognize the amino-terminal alpha 1-alpha 2 domain of Q6 fusion products. This recognition can be blocked by anti-Qa-2a alloantiserum and monoclonal antibodies reactive with the alpha 3-beta 2-microglobulin portion of the Q6 hybrids. We propose that the structural requirements for the anti-Qa-2a cytotoxic T lymphocyte-specific epitopes on target molecules are the same as for anti-H-2-alloreactive cytotoxic T lymphocyte determinants on transplantation antigens and that the mechanism of target recognition is similar in both cases. This interpretation is consistent with the following structural similarities found in both categories of class I molecules: (a) Kd and Q6 alpha 1-alpha 2 domains share serologically defined epitopes.(ABSTRACT TRUNCATED AT 400 WORDS)

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