scholarly journals Macrophage activation: increased ingestion of IgG-coated erythrocytes after administration of interferon inducers to mice.

1978 ◽  
Vol 147 (2) ◽  
pp. 593-598 ◽  
Author(s):  
S I Hamburg ◽  
R E Manejias ◽  
M Rabinovitch
Keyword(s):  
Macrophage Activation ◽  
Peritoneal Macrophages ◽  
Response Curves ◽  
Polycytidylic Acid ◽  
Vesicular Stomatitis ◽  
Macrophage Populations ◽  
And Control ◽  
Mouse Peritoneal Macrophages

In vitro phagocytosis of IgG-opsonized sheep erythrocytes (EA) was used to measure the in vivo activation of mouse peritoneal macrophages. Uptake of EA as enhanced by the extraperitoneal administration of Newcastle disease virus, vesicular stomatitis virus, tilorone or polyinosinic-polycytidylic acid. Ingestion of EA was similarly stimulated by lipopolysaccharide or killed Corynebacterium parvum. Dose-response curves relating concentrations of IgG to phagocytosis were parallel for both treated and control animals. This indicates that the heterogeneity of the macrophage populations did not change and that the overall populations were activated with respect to phagocytic ability. Numbers of macrophages were not increased (except in C. parvum-treated mice), suggesting that resident, rather than newly recruited macrophages, were activated by the different agents.

scholarly journals Proliferative capacity of mouse peritoneal macrophages in vitro.

1978 ◽  
Vol 147 (4) ◽  
pp. 1253-1266 ◽  
Author(s):  
B A van der Zeijst ◽  
C C Stewart ◽  
S Schlesinger
Keyword(s):  
Cell Cycle ◽  
Macrophage Activation ◽  
Peritoneal Macrophages ◽  
Precursor Cell ◽  
Proliferative Capacity ◽  
Nucleotidase Activity ◽  
Daughter Cells ◽  
Macrophage Populations ◽  
Mouse Peritoneal Macrophages

Thioglycolate-stimulated mouse peritoneal macrophages cultured in the presence of macrophage growth factor (MGF) will continue to proliferate when they are removed from culture dishes with the local anesthetic lidocaine and subcultured. The number of times the cells can be subcultured and remain in a proliferative state is dependent on the number of previous cell divisions. One precursor cell (colony-forming cell) yields about 2.6 X 10(4) daughter cells. When MGF is removed from actively proliferating macrophages, they leave the cell cycle and enter a "resting" condition. When MGF is readded, cells reenter the cell cycle and proliferate with the same doubling time as if MGF had not been removed. Membrane 5'-nucleotidase activity was used as a probe to identify the state of macrophage activation. Proliferating macrophage populations had significantly higher enzyme levels than stimulated macrophages cultured without MGF. These enzymes levels were, however, lower than those found for resident (unstimulated) macrophages.

scholarly journals Macrophage oxygen-dependent antimicrobial activity. III. Enhanced oxidative metabolism as an expression of macrophage activation.

1980 ◽  
Vol 152 (6) ◽  
pp. 1596-1609 ◽  
Author(s):  
H W Murray ◽  
Z A Cohn
Keyword(s):  
Antimicrobial Activity ◽  
Oxidative Metabolism ◽  
Macrophage Activation ◽  
Systemic Infection ◽  
Peritoneal Macrophages ◽  
H2o2 Production ◽  
Oxygen Intermediates ◽  
Cultivation In Vitro

The capacity of 15 separate populations of mouse peritoneal macrophages to generate and release H2O2 (an index of oxidative metabolism) was compared with their ability to inhibit the intracellular replication of virulent Toxoplasma gondii. Resident macrophages and those elicited by inflammatory agents readily supported toxoplasma multiplication and released 4-20X less H2O2 than macrophages activated in vivo by systemic infection with Bacille Calmette-Guérin or T. gondii, or by immunization with Corynebacterium parvum. Immunologically activated cells consistently displayed both enhanced H2O2 production and antitoxoplasma activity. Exposure to lymphokines generated from cultures of spleen cells from T. gondii immune mice and toxoplasma antigen preserved both the antitoxoplasma activity and the heightened H2O2 release of toxoplasma immune and immune-boosted macrophages, which otherwise were lost after 48-72 h of cultivation. In vitro activation of resident and chemically-elicited cells by 72 h of exposure to mitogen- and antigen-prepared lymphokines, conditions that induce trypanocidal (5) and leishmanicidal activity (14), stimulated O2- and H2O2 release, and enhanced nitroblue tetrazolium reduction in response to toxoplasma ingestion. Such treatment, however, failed to confer any antitoxoplasma activity, indicating that intracellular pathogens may vary in their susceptibility to macrophage microbicidal mechanisms, including specific oxygen intermediates. In contrast, cocultivating normal macrophages with lymphokine plus heart infusion broth for 18H rendered these cells toxoplasmastatic. This in vitro-acquired activity was inhibited by scavengers of O2-, H2O2, OH., and 1O2, demonstrating a role for oxidative metabolites in lymphokine-induced enhancement of macrophage antimicrobial activity. These findings indicate that augmented oxidative metabolism is an consistent marker of macrophage activation, and that oxygen intermediates participate in the resistance of both in vivo- and vitro-activated macrophages toward the intracellular parasite, T. gondii.

scholarly journals A Critical Role of Activin A in Maturation of Mouse Peritoneal Macrophages in vitro and in vivo

2009 ◽  
Vol 6 (5) ◽  
pp. 387-392 ◽  
Author(s):  
Yinan Wang ◽  
Xueling Cui ◽  
Guixiang Tai ◽  
Jingyan Ge ◽  
Nan Li ◽  
...  
Keyword(s):  
Critical Role ◽  
Peritoneal Macrophages ◽  
Activin A ◽  
Mouse Peritoneal Macrophages

scholarly journals Constitutive secretion of cystatin C (gamma-trace) by monocytes and macrophages and its downregulation after stimulation.

1987 ◽  
Vol 166 (6) ◽  
pp. 1912-1917 ◽  
Author(s):  
A H Warfel ◽  
D Zucker-Franklin ◽  
B Frangione ◽  
J Ghiso
Keyword(s):  
Cell Lines ◽  
Cystatin C ◽  
Peritoneal Macrophages ◽  
Inflammatory Conditions ◽  
Monocytes And Macrophages ◽  
Constitutive Secretion ◽  
Established Cell ◽  
Mouse Peritoneal Macrophages

Cystatin C (gamma-trace) was found to be a constitutively secreted protein of isolated human monocytes and mouse peritoneal macrophages, as well as the histiocytic lymphoma cell lines U937, P388D.1, and J774. This proteinase inhibitor is not uniquely secreted by monocytes/macrophages, but was also identified in the conditioned media from several primary cells, including brain cells, and diverse established cell lines. In vitro treatment of resident mouse peritoneal macrophages with either LPS or IFN-gamma caused a downregulation in cystatin C secretion. Elaboration of this protein was also diminished by macrophages that had been stimulated by thioglycollate in vivo, and treatment of these cells with LPS led to further decline. It is suggested that, under some inflammatory conditions, downregulation of cystatin C may contribute to tissue pathology.

Activin A down-regulates the phagocytosis of lipopolysaccharide-activated mouse peritoneal macrophages in vitro and in vivo

2009 ◽  
Vol 255 (1-2) ◽  
pp. 69-75 ◽  
Author(s):  
Jing Zhou ◽  
Guixiang Tai ◽  
Haiyan Liu ◽  
Jingyan Ge ◽  
Ye Feng ◽  
...  
Keyword(s):  
Peritoneal Macrophages ◽  
Activin A ◽  
Mouse Peritoneal Macrophages

scholarly journals Increased production of superoxide anion by macrophages exposed in vitro to muramyl dipeptide or lipopolysaccharide.

1980 ◽  
Vol 151 (1) ◽  
pp. 101-114 ◽  
Author(s):  
M J Pabst ◽  
R B Johnston
Keyword(s):  
Superoxide Anion ◽  
Muramyl Dipeptide ◽  
Peritoneal Macrophages ◽  
Generating Capacity ◽  
Nonadherent Cell ◽  
Mouse Peritoneal Macrophages ◽  
Nonadherent Cells ◽  
Oxygen Metabolites

After in vitro exposure to lipopolysaccharide (LPS) or muramyl dipeptide (MDP), cultured resident mouse peritoneal macrophages were primed to display enhanced generation of superoxide anion (O2-) in response to stimulation by phorbol myristate acetate (PMA) or opsonized zymosan. Priming with LPS (1 microgram/ml) produced a sevenfold enhancement of PMA-stimulated O2- generation; priming was detected within 30 min and persisted for at least 4 d. Exposure to MDP (1 muM) primed the macrophages to double their O2- release; the response was first observed after 4 h and persisted for at least 3 d. The priming response was not observed with stereoisomers of MDP, which are inactive as adjuvants. LPS and MDP appeared to work directly on the macrophages rather than indirectly by interacting with adherent lymphocytes: (a) Addition of nonadherent cell populations that contained lymphocytes had no effect on the response. (b) The response was normal with cells from nude mice, which lack mature T lymphocytes. (c) Macrophages from C3H/HeJ mice, whose B lymphocytes fail to respond to LPS, were weak in their response to priming LPS; the addition of normal (C3Heb/FeJ) nonadherent cells had no effect on this weak response. (d) The macrophage-like cell line J774.1 also showed enhanced O2--generating capacity after a 4-h exposure to LPS or MDP. The O2--generating capacity of macrophages primed with LPS in vitro was equivalent to that previously observed with cells elicited in vivo by injection of LPS or activated by infection with Bacille Calmette-Guérin. The data suggest that previous exposure to bacterial products could prime macrophages to respond with increased production of toxic oxygen metabolites on contact with invading microorganisms or tumor cells.

Evaluation of Immune Function of Mycelium Fermentation of Hirsutella sinensisin In Vitro and In Vivo

2020 ◽  
Vol 12 (11) ◽  
pp. 1337-1343
Author(s):  
Bing Liu ◽  
De Ding ◽  
Ping Zhao ◽  
Xinguo Zhang ◽  
Zhenji Tian ◽  
...  
Keyword(s):  
Biological Activity ◽  
Nk Cells ◽  
Peritoneal Macrophages ◽  
Mouse Spleen ◽  
Spleen Lymphocytes ◽  
Chicken Erythrocytes ◽  
Functional Components ◽  
Mouse Peritoneal Macrophages

In order to confirm whether HSFL (the fermented liquor of mycelia of Hirsutella sinensis) still contains cordycepin, Cordyceps polysaccharide and other functional components, and has the functions of anti-oxidation, tumor inhibition and immunity enhancement, the biological activity of HSFL In Vivo and in vitro was studied in this study. The transformation ability of mouse spleen lymphocytes induced by ConA, the activity of NK cells in mouse spleen, the delayed allergic reaction induced by DNFB and the phagocytosis of chicken red cells by mouse peritoneal macrophages were studied. The results showed that when the concentration of HSFL was 0.5972 mg/kg and 1.1944 mg/kg, the transformation of lymphocytes induced by ConA and the activity of NK cells were significantly increased. HSFL also can significantly improve DNFB induced anaphylaxis in mice and phagocytosis of chicken erythrocytes by peritoneal macrophages in mice when the dose of HSFL is 50 mg/kg and 75 mg/kg, indicating that HSFL has the biological activity of enhancing immunity in vitro and In Vivo.

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