scholarly journals Redistribution of a major cell surface glycoprotein during cell movement.

1984 ◽  
Vol 99 (5) ◽  
pp. 1613-1623 ◽  
Author(s):  
K Jacobson ◽  
D O'Dell ◽  
B Holifield ◽  
T L Murphy ◽  
J T August
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Surface ◽  
Cell Movement ◽  
Leading Edge ◽  
Surface Glycoprotein ◽  
Direct Immunofluorescence ◽  
Surface Distribution ◽  
Cell Surface Glycoprotein ◽  
Cell Functions ◽  
Motile Cells

The distribution in living cells of an 80,000-dalton major cell surface glycoprotein of murine fibroblasts has been studied by use of monoclonal antibodies. The presence of the molecule throughout the plasma membrane and on the substrate attached surface of the cell was demonstrated by immunofluorescence. Cell growth kinetics were not altered and the cells remained motile in the presence of the antibody. The uniform distribution of the direct immunofluorescence stain persisted for long periods (greater than 100 h), which indicates that the fluorescent monoclonal antibodies may be used to trace antigen surface distribution during cell functions. In motile cells, but not G0 or confluent cells, the degree of fluorescent staining decreased toward the leading edge; this gradient increased markedly during the time that the antibody was bound to the cells. However, the gradation was not seen with the lipid probe, dihexadecylindocarbocyanine. The antigen was "patched" only by the application of a second antibody directed to the rat monoclonal antibody and the relationships of these patches to the underlying cytoskeleton were characterized.

Human T-cell leukaemia-associated cell surface glycoprotein gp37: Studies with three monoclonal antibodies and a rabbit antiserum

1986 ◽  
Vol 23 (6) ◽  
pp. 569-580 ◽  
Author(s):  
Ben K. Seon ◽  
Takashi Fukukawa ◽  
Anne L. Jackson ◽  
David Chervinsky ◽  
Cameron K. Tebbi ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
T Cell ◽  
Cell Surface ◽  
Rabbit Antiserum ◽  
Surface Glycoprotein ◽  
Cell Leukaemia ◽  
Cell Surface Glycoprotein ◽  
Human T Cell

Monoclonal Antibodies to a Human Islet Cell Surface Glycoprotein: 4F2 and LC7-2*

Endocrinology ◽  
1987 ◽  
Vol 120 (6) ◽  
pp. 2240-2244 ◽  
Author(s):  
S. SRIKANTA ◽  
M. TELEN ◽  
J. T. POSILLICO ◽  
R. DOLINAR ◽  
K. KRISCH ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Surface ◽  
Islet Cell ◽  
Human Islet ◽  
Surface Glycoprotein ◽  
Cell Surface Glycoprotein

scholarly journals Expression of the human monocyte membrane antigen gp55 by murine fibroblasts after DNA-mediated gene transfer

Blood ◽  
1987 ◽  
Vol 69 (3) ◽  
pp. 886-892 ◽  
Author(s):  
RA Ashmun ◽  
SC Peiper ◽  
MB Rebentisch ◽  
AT Look
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Surface ◽  
Dna Sequences ◽  
Cell Sorting ◽  
Surface Glycoprotein ◽  
Human Monocytes ◽  
Blood Monocytes ◽  
Gene Encoding ◽  
Cell Surface Glycoprotein ◽  
Human Dna

Abstract Human DNA sequences that contain the gene encoding gp55, a cell surface glycoprotein expressed exclusively on mature human monocytes and monocytic leukemia cells, were isolated in a mouse genetic background. DNA from mature human monocytes was cotransfected with DNA from a molecularly cloned feline sarcoma virus containing the v-fms oncogene into NIH-3T3 cells. Transformed mouse fibroblasts that expressed gp55, based on their reactivity with the MY4, B44.1, or LeuM3 monoclonal antibodies, were selected by fluorescence-activated cell sorting. Regardless of which antibody was used for selection, equivalent binding of all three antibodies was observed for positive transformants. Secondary and tertiary mouse cell transformants were obtained after additional rounds of transfection and cell sorting with the use of DNA from primary and then secondary transformants. Southern blot analysis of the cellular DNA from two independently derived tertiary subclones revealed a limited complement of human sequences, thus indicating that the gene encoding gp55 is included in fewer than 50 kilobases of human DNA. Independently derived tertiary subclones displayed concordant patterns of reactivity with 13 monocyte-specific monoclonal antibodies, thus indicating that each recognized an epitope on the product (gp55) of a single human gene. The 55-kilodalton cell surface polypeptide was specifically immunoprecipitated with a representative monoclonal antibody, 26if, from lysates of enzymatically radioiodinated peripheral blood monocytes and tertiary transformants. We conclude that gp55 is highly immunogenic and that a large number of independently derived monoclonal antibodies specific for human monocytes react with epitopes on this one molecule.

scholarly journals EMA: A developmentally regulated cell-surface glycoprotein of CNS neurons that is concentrated at the leading edge of growth cones

1992 ◽  
Vol 194 (4) ◽  
pp. 311-325 ◽  
Author(s):  
Nikki L. Baumrind ◽  
David Parkinson ◽  
Denise B. Wayne ◽  
John E. Heuser ◽  
Alan L. Pearlman
Keyword(s):  
Cell Surface ◽  
Leading Edge ◽  
Growth Cones ◽  
Surface Glycoprotein ◽  
Developmentally Regulated ◽  
Cell Surface Glycoprotein ◽  
Cns Neurons

scholarly journals Expression of the human monocyte membrane antigen gp55 by murine fibroblasts after DNA-mediated gene transfer

Blood ◽  
1987 ◽  
Vol 69 (3) ◽  
pp. 886-892
Author(s):  
RA Ashmun ◽  
SC Peiper ◽  
MB Rebentisch ◽  
AT Look
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Surface ◽  
Dna Sequences ◽  
Cell Sorting ◽  
Surface Glycoprotein ◽  
Human Monocytes ◽  
Blood Monocytes ◽  
Gene Encoding ◽  
Cell Surface Glycoprotein ◽  
Human Dna

Human DNA sequences that contain the gene encoding gp55, a cell surface glycoprotein expressed exclusively on mature human monocytes and monocytic leukemia cells, were isolated in a mouse genetic background. DNA from mature human monocytes was cotransfected with DNA from a molecularly cloned feline sarcoma virus containing the v-fms oncogene into NIH-3T3 cells. Transformed mouse fibroblasts that expressed gp55, based on their reactivity with the MY4, B44.1, or LeuM3 monoclonal antibodies, were selected by fluorescence-activated cell sorting. Regardless of which antibody was used for selection, equivalent binding of all three antibodies was observed for positive transformants. Secondary and tertiary mouse cell transformants were obtained after additional rounds of transfection and cell sorting with the use of DNA from primary and then secondary transformants. Southern blot analysis of the cellular DNA from two independently derived tertiary subclones revealed a limited complement of human sequences, thus indicating that the gene encoding gp55 is included in fewer than 50 kilobases of human DNA. Independently derived tertiary subclones displayed concordant patterns of reactivity with 13 monocyte-specific monoclonal antibodies, thus indicating that each recognized an epitope on the product (gp55) of a single human gene. The 55-kilodalton cell surface polypeptide was specifically immunoprecipitated with a representative monoclonal antibody, 26if, from lysates of enzymatically radioiodinated peripheral blood monocytes and tertiary transformants. We conclude that gp55 is highly immunogenic and that a large number of independently derived monoclonal antibodies specific for human monocytes react with epitopes on this one molecule.

scholarly journals The use of recombinant vaccinia virus to generate monoclonal antibodies against the cell-surface glycoprotein endoglin

FEBS Letters ◽  
1997 ◽  
Vol 413 (2) ◽  
pp. 265-268 ◽  
Author(s):  
Alfonso Luque ◽  
Carlos Cabañas ◽  
Ulla Raab ◽  
Ainhoa Letamendı́a ◽  
Eduardo Páez ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Surface ◽  
Vaccinia Virus ◽  
Recombinant Vaccinia Virus ◽  
Surface Glycoprotein ◽  
Cell Surface Glycoprotein ◽  
Recombinant Vaccinia
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