scholarly journals Immunocytochemical localization of microtubule-associated protein 1 in rat cerebellum using monoclonal antibodies.

1984 ◽  
Vol 98 (2) ◽  
pp. 777-781 ◽  
Author(s):  
G Huber ◽  
A Matus
Keyword(s):  
Monoclonal Antibodies ◽  
Threshold Level ◽  
Specific Protein ◽  
Tissue Preparation ◽  
Rat Cerebellum ◽  
Different Types ◽  
Apparent Concentration ◽  
Golgi Neurons ◽  
Brain Microtubules ◽  
Different Levels

Immunohistochemical staining with monoclonal antibodies showed that microtubule-associated protein 1 (MAP1) has a restricted cellular distribution in the rat cerebellum. Anti-MAP1 staining was found only in neurons, where it was much stronger in dendrites than in axons. There were striking variations in the apparent concentration of MAP1 in different classes of neurons. Purkinje cells were the most strongly labeled, while granule cell neurons gave a faint, threshold-level reaction with the antibody. The reaction of Golgi neurons was intermediate between these two extremes. Equivalent results were obtained using two different methods of tissue preparation. Thus MAP1 appears to be a neuron-specific protein that is highly concentrated in dendrites and occurs at markedly different levels in different types of neurons. These observations provide further indications of heterogeneity among brain microtubules.

scholarly journals Snail mucus from the mantle and foot of two land snails, Lissachatina fulica and Hemiplecta distincta, exhibits different protein profile and biological activity

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Nattaphop Noothuan ◽  
Kantamas Apitanyasai ◽  
Somsak Panha ◽  
Anchalee Tassanakajon
Keyword(s):  
Biological Activities ◽  
Protein Pattern ◽  
Protein Profile ◽  
Antibacterial Properties ◽  
Biological Properties ◽  
Specific Protein ◽  
Land Snails ◽  
Snail Species ◽  
Significant Difference ◽  
Different Types

Abstract Objective Snails secrete different types of mucus that serve several functions, and are increasingly being exploited for medical and cosmetic applications. In this study, we explored the protein pattern and compared the biological properties of the mucus secreted from the mantle collar and foot of two snail species, Lissachatina fulica and Hemiplecta distincta. Result Protein profile showed a different pattern between the two species and between the two secretory parts. The mantle-specific protein bands were further characterized and among them was an antibacterial protein, achacin. Accordingly, the mucus from the mantle exhibited the higher antibacterial activity than that from the foot in both snail species. The mucus from H. distincta, first reported here, also showed antibacterial properties, but with a lower activity compared to that for L. fulica. Snail mucus also exhibited anti-tyrosinase activity and antioxidant activity but with no significant difference between the foot and mantle mucus. These results indicate some different protein compositions and biological activities of snail slime from the mantle and foot, which might be associated with their specific functions in the animal and are useful for medical applications.

scholarly journals Monoclonal Antibodies as Neurological Therapeutics

2021 ◽  
Vol 14 (2) ◽  
pp. 92
Author(s):  
Panagiotis Gklinos ◽  
Miranta Papadopoulou ◽  
Vid Stanulovic ◽  
Dimos D. Mitsikostas ◽  
Dimitrios Papadopoulos
Keyword(s):  
Monoclonal Antibodies ◽  
Molecular Mechanisms ◽  
Neurological Diseases ◽  
Therapeutic Agents ◽  
Medical Specialties ◽  
Specific Effects ◽  
Different Types ◽  
Neurological Conditions ◽  
Disease Pathways

Over the last 30 years the role of monoclonal antibodies in therapeutics has increased enormously, revolutionizing treatment in most medical specialties, including neurology. Monoclonal antibodies are key therapeutic agents for several neurological conditions with diverse pathophysiological mechanisms, including multiple sclerosis, migraines and neuromuscular disease. In addition, a great number of monoclonal antibodies against several targets are being investigated for many more neurological diseases, which reflects our advances in understanding the pathogenesis of these diseases. Untangling the molecular mechanisms of disease allows monoclonal antibodies to block disease pathways accurately and efficiently with exceptional target specificity, minimizing non-specific effects. On the other hand, accumulating experience shows that monoclonal antibodies may carry class-specific and target-associated risks. This article provides an overview of different types of monoclonal antibodies and their characteristics and reviews monoclonal antibodies currently in use or under development for neurological disease.

Accessing offshoring advantages: what and how to offshore

2015 ◽  
Vol 8 (2/3) ◽  
pp. 262-283 ◽  
Author(s):  
Alona Mykhaylenko ◽  
Ágnes Motika ◽  
Brian Vejrum Waehrens ◽  
Dmitrij Slepniov
Keyword(s):  
Performance Outcomes ◽  
Offshore Outsourcing ◽  
Strategic Decisions ◽  
Content Type ◽  
Different Types ◽  
Captive Offshoring ◽  
Performance Results ◽  
Governance Modes ◽  
Different Levels ◽  
Do So

Purpose – The purpose of this paper is to advance the understanding of factors that affect offshoring performance results. To do so, this paper focuses on the access to location-specific advantages, rather than solely on the properties of the offshoring company, its strategy or environment. Assuming that different levels of synergy may exist between particular offshoring strategic decisions (choosing offshore outsourcing or captive offshoring and the type of function) and different offshoring advantages, this work advocates that the actual fact of realization of certain offshoring advantages (getting or not getting access to them) is a more reliable predictor of offshoring success. Design/methodology/approach – A set of hypotheses derived from the extant literature is tested on the data from a quantitative survey of 1,143 Scandinavian firms. Findings – The paper demonstrates that different governance modes and types of offshored function indeed provide different levels of access to different types of location-specific offshoring advantages. This difference may help to explain the ambiguity of offshoring initiatives performance results. Research limitations/implications – Limitations of the work include using only the offshoring strategy elements and only their limited variety as factors potentially influencing access to offshoring advantages. Also, the findings are limited to Scandinavian companies. Originality/value – The paper introduces a new concept of access, which can help to more reliably predict performance outcomes of offshoring initiatives. Recommendations are also provided to practitioners dealing with offshoring initiatives.

The initial synthesis of haemoglobin in de-embryonated chick blastoderms.

Development ◽  
1964 ◽  
Vol 12 (4) ◽  
pp. 609-619
Author(s):  
Anna Hell
Keyword(s):  
Protein Synthesis ◽  
Deoxyribonucleic Acid ◽  
Primitive Streak ◽  
Specific Protein ◽  
In Vitro System ◽  
Embryonic Tissues ◽  
Different Types ◽  
Excellent Tool ◽  
Made In

Enormous progress has been made in the last few years towards the elucidation of the mechanism of protein synthesis, and great interest is centred on the steps leading to cellular differentiation and specific protein synthesis. We know that genetic information is passed on from one generation of cells to the next by deoxyribonucleic acid (DNA), and that this material directs all protein synthesis by the intermediary of the different types of ribonucleic acid (RNA). A simple in vitro system described by O'Brien (1959) seemed to offer an excellent tool for the study of the differentiation of the blood islands, and the initial formation of a well-known protein, haemoglobin (Hb), in chick embryonic tissues. After de-embryonation, chick blastoderms, from the stage of primitive streak onwards, can be cultured in vitro on a saline agar medium supplemented with glucose.

Sticks and Stones May Break My Bones . . . Reasoning About Illness Causality and Body Functioning in Children Who Have a Chronic Illness

PEDIATRICS ◽  
1991 ◽  
Vol 88 (3) ◽  
pp. 608-619
Author(s):  
Ellen C. Perrin ◽  
Aline G. Sayer ◽  
John B. Willett
Keyword(s):  
Chronic Illness ◽  
Age Differences ◽  
Conceptual Development ◽  
Seizure Disorder ◽  
Healthy Children ◽  
Cognitive Reasoning ◽  
Different Types ◽  
Reasoning Skills ◽  
Experience Of Illness ◽  
Different Levels

Children's concepts about illness causality and bodily functioning change in a predictable way with advancing age. Differences in the understanding of these concepts in healthy children vs children with a chronic illness have not been clearly delineated. This study included 49 children with a seizure disorder, 47 children with an orthopaedic condition, and 96 healthy children, all with normal intelligence and ranging in age from 5 to 16 years. It demonstrates systematic differences in children's general reasoning skills and in their understanding of concepts about illness causality and bodily functioning, as a function of their age and experience of illness. At all ages, children who had a condition with orthopaedic involvement reported less sophisticated general reasoning and concepts about illness than did healthy children; children with a seizure disorder reported similar general reasoning skills to those of healthy children, but considerably less sophisticated concepts about illness. children's concepts about body functioning did not differ as a function of the presence of a chronic illness. When their different levels of general cognitive reasoning were statistically controlled, children with a chronic illness had somewhat more sophisticated concepts about bodily functioning than did healthy children. Differences in conceptual development among children with different types of illnesses lead to interesting speculations with regard to the effects of particular illness characteristics on children's cognitive development.

scholarly journals The Parietal Cortex in Sensemaking: The Dissociation of Multiple Types of Spatial Information

2013 ◽  
Vol 2013 ◽  
pp. 1-14 ◽  
Author(s):  
Yanlong Sun ◽  
Hongbin Wang
Keyword(s):  
Parietal Cortex ◽  
Spatial Information ◽  
Mathematical Thinking ◽  
Hierarchical Organization ◽  
Levels Of Abstraction ◽  
Perceptual Analysis ◽  
Different Types ◽  
Sensemaking Process ◽  
Network Simulations ◽  
Different Levels

According to the data-frame theory, sensemaking is a macrocognitive process in which people try to make sense of or explain their observations by processing a number of explanatory structures called frames until the observations and frames become congruent. During the sensemaking process, the parietal cortex has been implicated in various cognitive tasks for the functions related to spatial and temporal information processing, mathematical thinking, and spatial attention. In particular, the parietal cortex plays important roles by extracting multiple representations of magnitudes at the early stages of perceptual analysis. By a series of neural network simulations, we demonstrate that the dissociation of different types of spatial information can start early with a rather similar structure (i.e., sensitivity on a common metric), but accurate representations require specific goal-directed top-down controls due to the interference in selective attention. Our results suggest that the roles of the parietal cortex rely on the hierarchical organization of multiple spatial representations and their interactions. The dissociation and interference between different types of spatial information are essentially the result of the competition at different levels of abstraction.

Who Wins and Who Loses in the Provincial Courts of Appeal? A Statistical Analysis, 1920–1990

1994 ◽  
Vol 9 (2) ◽  
pp. 21-40 ◽  
Author(s):  
Peter McCormick
Keyword(s):  
United States ◽  
Statistical Analysis ◽  
Great Britain ◽  
Data Base ◽  
The United States ◽  
Different Types ◽  
Party Capability ◽  
Time Periods ◽  
Appeal Courts ◽  
Different Levels

AbstractGiven the visibility and obvious importance of judicial power in the age of the Charter, it is important to develop the conceptual vocabulary for desribing and assessing this power. One such concept that has been applied to the study of appeal courts in the United States and Great Britain is “party capability”, a theory which suggests that different types of litigant will enjoy different levels of success as both appellant and respondent. Using a data base derived from the reported decisions of the provincial courts of appeal for the second and seventh year of each decade since the 1920s, this article applies party capability theory to the performance of the highest courts of the ten provinces; comparisons are attempted across regions and across time periods, as well as with the findings of similar studies of American and British courts.

Reactions of human antibodies with CR1 immobilised by mouse monoclonal antibody E11 Red cell phenotype Murine MAb Human anti- Absorbance Ratio Kn(a+) ] Kn(a-) E11 Kna 0.755 0.195 4:1 McC(a+) 0.538 McC(a-) E11 McC 0.136 4:1 Yk(a+) 0.315 Yk(a-) E11 Yka 0.120 26:1 Sl(a+) 0.342 Sl(a-) E11 Sla 0.074 4.6:1 Cs(a+) 0.139 Cs(a-) E11 Cs 0.108 Mapping relative positions of antigens on a specific protein When several murine monoclonal antibodies to different epitopes on the same protein are available, MAIEA can be used to study the relative position of antigens on that protein. This application of MAIEA depends on mutual inhibition of murine monoclonal antibodies and human antibodies. A negative result is obtained when human and monoclonal antibodies compete for the same epitope, or bind to very closely located epitopes, so no tri-molecular complex is produced. Several monoclonal antibodies to the Kell protein have been used in MAIEA to study the relationships of the Kell system antigens [10]. The decay accelerating factor DAF, CD55, is detected by several monoclonal antibodies. Three antibodies BRIC 230, BRIC 110 and BRIC 216 were known from competitive binding assays to bind to different short consensus repeats (SCR) [11]. So three of the four SCRs of the DAF molecule were positively identified (Table II). Strong positive reactions were observed with all three BRIC antibodies and anti-Cr3, anti-WES8, and anti-WESb showing that MAIEA is a useful techique for studying this system [12]. The results showed that Cr8, WESa, and WESb are not on the first three SCRs and must

1995 ◽  
pp. 190-190
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Molecular Complex ◽  
Specific Protein ◽  
Cell Phenotype ◽  
Binding Assays ◽  
Human Antibodies ◽  
Short Consensus Repeats ◽  
Murine Monoclonal Antibodies ◽  
Competitive Binding Assays

scholarly journals Utilization of Salicornia fruticosa herb for producing antioxidants

2014 ◽  
Vol 49 (1) ◽  
pp. 53-58 ◽  
Author(s):  
EM Elsebaie ◽  
SYA Elsanat ◽  
MS Gouda ◽  
KM Elnemr
Keyword(s):  
Corn Oil ◽  
Natural Antioxidants ◽  
Polyphenolic Compounds ◽  
Synthetic Antioxidant ◽  
Oxidative Rancidity ◽  
Total Polyphenols ◽  
Keeping Quality ◽  
Different Types ◽  
Phenolic Extract ◽  
Different Levels

The present work was aimed to study the effect of extracted phenolic compounds from Salicornia air part by several solvents as natural antioxidants on preservation of corn oil comparing with synthetic antioxidant (TBA) on the oil stability against oxidative rancidity during storage at 70 °C for 5 days. The results indicate that the best solvent for extracting polyphenolic compounds was methanol followed by ethanol, chloroform and water. HPLC analysis for the total polyphenols extracted from the air part of salicornia fruticosa indicated to presence high percentages of Pyrogallol, Ellagic, B-OH Benzoic and Catechin. The extracted phenolic acids were tested against corn oil keeping quality. Results show that peroxide value and TBA values of corn oil that treated by different types of extracts at different levels were lower than control. Keywords: Salicornia fruticosa; DPPH; Corn oil; Phenolic extract. DOI: http://dx.doi.org/10.3329/bjsir.v49i1.18856 Bangladesh J. Sci. Ind. Res. 49(1), 53-58, 2014

Sign in / Sign up

Export Citation Format

Share Document