scholarly journals Polymorphism of actin paracrystals induced by polylysine.

1982 ◽  
Vol 93 (2) ◽  
pp. 452-458 ◽  
Author(s):  
W E Fowler ◽  
U Aebi

We describe a method for the induction of different polymorphic forms of actin filament paracrystals. This polymorphism is probably based on differences in the stagger and/or polarity of adjacent filaments in single-layered paracrystals and by superposition of different layers in multilayered paracrystals. The helical parameters defining the filament geometry are indistinguishable for the different polymorphic forms observed and for the four different actins used. Analysis of these paracrystals, some of which are ordered to better than 2.5 nm, should provide a reference structure suitable for alignment and orientation within the actin filament of high resolution models of the actin monomer obtained from crystal data.

2010 ◽  
Vol 138 (9) ◽  
pp. 3418-3433 ◽  
Author(s):  
Tanja Weusthoff ◽  
Felix Ament ◽  
Marco Arpagaus ◽  
Mathias W. Rotach

Abstract High-resolution numerical weather prediction (NWP) models produce more detailed precipitation structures but the real benefit is probably the more realistic statistics gained with the higher resolution and not the information on the specific grid point. By evaluating three model pairs, each consisting of a high-resolution NWP system resolving convection explicitly and its low-resolution-driving model with parameterized convection, on different spatial scales and for different thresholds, this paper addresses the question of whether high-resolution models really perform better than their driving lower-resolution counterparts. The model pairs are evaluated by means of two fuzzy verification methods—upscaling (UP) and fractions skill score (FSS)—for the 6 months of the D-PHASE Operations Period and in a highly complex terrain. Observations are provided by the Swiss radar composite and the evaluation is restricted to the area covered by the Swiss radar stations. The high-resolution models outperform or equal the performance of their respective lower-resolution driving models. The differences between the models are significant and robust against small changes in the verification settings. An evaluation based on individual months shows that high-resolution models give better results, particularly with regard to convective, more localized precipitation events.


2021 ◽  
Vol 13 (19) ◽  
pp. 3997
Author(s):  
Shuyan Zhang ◽  
Yong Ma ◽  
Fu Chen ◽  
Erping Shang ◽  
Wutao Yao ◽  
...  

Clouds play an important role in the energy and moisture cycle of the earth–atmosphere system, which affects many important processes in nature and human societies. However, there are very few fine-grained and high-precision global cloud climatology data available for high-resolution models. In this paper, we produced a fine-grained (1 km resolution) global land cloud climatology (GLHCC) report based on MOD09 cloud masks from 2001 to 2016, with a temporal resolution of 10 days. The two improvements (short-wave infrared and Band 2/6 ratio threshold method) on the original MOD09 cloud mask have reduced the snow, ice, and bright areas mistakenly classified as clouds. The preliminary cloud products undergo the removal of orbital artifacts by Variational Stationary Noise Remover (VSNR) and the removal of abnormal albedo areas to generate the final cloud climatology data. The new product was directly validated by ground-based cloud observations collected from 3777 global weather stations. PATMOS-X from the Advanced Very High Resolution Radiometer (AVHRR) and MOD/MYD35 served as comparison products for consistency check of GLHCC. The assessment results show that GLHCC demonstrated a strong correlation with ground station observations, MOD/MYD35, and PATMOS-X. When the ground observations were taken as the truth value, GLHCC and MOD/MYD35 displayed higher accuracy than PATMOS-X. In most selected interested areas where the three behave differently, GLHCC matched the facts better than MOD/MYD35 and PATMOS-X. The GLHCC can well represent the cloud distribution over the past 16 years and will play an important role in the fine-grained demands of many aspects of nature and human society.


2018 ◽  
Author(s):  
Grigore Moldovan ◽  
Wolfgang Joachimi ◽  
Guillaume Boetsch ◽  
Jörg Jatzkowski ◽  
Frank Altman

Abstract This work presents advanced resistance mapping techniques based on Scanning Electron Microscopy (SEM) with nanoprobing systems and the related embedded electronics. Focus is placed on recent advances to reduce noise and increase speed, such as integration of dedicated in situ electronics into the nanoprobing platform, as well as an important transition from current-sensitive to voltagesensitive amplification. We show that it is now possible to record resistance maps with a resistance sensitivity in the 10W range, even when the total resistance of the mapped structures is in the range of 100W. A reference structure is used to illustrate the improved performance, and a lowresistance failure case is presented as an example of analysis made possible by these developments.


2013 ◽  
Vol 543 ◽  
pp. 35-38 ◽  
Author(s):  
Masaaki Futamoto ◽  
Tatsuya Hagami ◽  
Shinji Ishihara ◽  
Kazuki Soneta ◽  
Mitsuru Ohtake

Effects of magnetic material, coating thickness, and tip radius on magnetic force microscope (MFM) spatial resolution have been systematically investigated. MFM tips are prepared by using an UHV sputtering system by coating magnetic materials on non-magnetic Si tips employing targets of Ni, Ni-Fe, Co, Fe, Fe-B, and Fe-Pd. MFM spatial resolutions better than 9 nm have been confirmed by employing magnetic tips coated with high magnetic moment materials with optimized thicknesses.


PLoS ONE ◽  
2018 ◽  
Vol 13 (12) ◽  
pp. e0208586 ◽  
Author(s):  
Miro Janco ◽  
Till Böcking ◽  
Stanley He ◽  
Adelle C. F. Coster

2008 ◽  
Vol 136 (9) ◽  
pp. 3408-3424 ◽  
Author(s):  
Humphrey W. Lean ◽  
Peter A. Clark ◽  
Mark Dixon ◽  
Nigel M. Roberts ◽  
Anna Fitch ◽  
...  

Abstract With many operational centers moving toward order 1-km-gridlength models for routine weather forecasting, this paper presents a systematic investigation of the properties of high-resolution versions of the Met Office Unified Model for short-range forecasting of convective rainfall events. The authors describe a suite of configurations of the Met Office Unified Model running with grid lengths of 12, 4, and 1 km and analyze results from these models for a number of convective cases from the summers of 2003, 2004, and 2005. The analysis includes subjective evaluation of the rainfall fields and comparisons of rainfall amounts, initiation, cell statistics, and a scale-selective verification technique. It is shown that the 4- and 1-km-gridlength models often give more realistic-looking precipitation fields because convection is represented explicitly rather than parameterized. However, the 4-km model representation suffers from large convective cells and delayed initiation because the grid length is too long to correctly reproduce the convection explicitly. These problems are not as evident in the 1-km model, although it does suffer from too numerous small cells in some situations. Both the 4- and 1-km models suffer from poor representation at the start of the forecast in the period when the high-resolution detail is spinning up from the lower-resolution (12 km) starting data used. A scale-selective precipitation verification technique implies that for later times in the forecasts (after the spinup period) the 1-km model performs better than the 12- and 4-km models for lower rainfall thresholds. For higher thresholds the 4-km model scores almost as well as the 1-km model, and both do better than the 12-km model.


2020 ◽  
Vol 5 (1) ◽  
pp. 13-17
Author(s):  
György Zoltán Radnóczi ◽  
Zoltán Herceg ◽  
Tamás Rafael Kiss

AbstractVery accurate measurement of distances in the order of several µm is demonstrated on a single crystal Si sample by counting the lattice fringes on stitched high resolution TEM/STEM images. Stitching of TEM images commonly relies on correspondence points found in the image, however, the nearly perfect periodic nature of a lattice image renders such a procedure very unreliable. To overcome this difficulty artificial correspondence points are created on the sample using the electron beam. An accuracy better than 1% can be reached while measuring distances in the order of 1 µm. A detailed description of the process is provided, and its usability for accurately measuring large distances is discussed in detail.


2021 ◽  
Vol 12 ◽  
pp. 528
Author(s):  
Yoshimichi Sato ◽  
Toshiki Endo ◽  
Shingo Kayano ◽  
Hitoshi Nemoto ◽  
Kazuki Shimada ◽  
...  

Background: The subcallosal artery (ScA) is a single dominant artery arising from the anterior communicating artery. Its injury causes amnesia and cognitive disturbance. The conventional computed tomographic angiography (C-CTA) is a common evaluation method of the intracranial artery. However, to image tinny perforating arteries such as the ScA is technically demanding for C-CTA. The purpose of this study is to investigate whether the ultra-high-resolution CTA (UHR-CTA) could image the ScA better than C-CTA. UHR-CTA became available in clinical practice in 2017. Its novel features are the improvement of the detector system and a small X-ray focus. Methods: Between April 2019 and May 2020, 77 and 49 patients who underwent intracranial UHR-CTA and C-CTA, respectively, were enrolled in this study. Two board-certified neurosurgeons participated as observers to identify the ScA based on UHR-CTA and C-CTA images. Results: UHR-CTA and C-CTA detected the ScA in 56–58% and 30–40% of the patients, respectively. In visualization of the ScA, UHR-CTA was better than C-CTA (P < 0.05, Fisher’s exact test). Between the two observers, the Cohen’s kappa coefficient was 0.77 for UHR-CTA and 0.78 for C-CTA. Conclusions: UHR-CTA is a simple and accessible method to evaluate intracranial vasculature. Visualization of the ScA with UHR-CTA was better than that with C-CTA. The high quality of UHR-CTA could provide useful information in the neurosurgery field.


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