scholarly journals Epidermal cells adhere preferentially to type IV (basement membrane) collagen.

1979 ◽  
Vol 80 (1) ◽  
pp. 197-202 ◽  
Author(s):  
J C Murray ◽  
G Stingl ◽  
H K Kleinman ◽  
G R Martin ◽  
S I Katz
Keyword(s):  
Basement Membrane ◽  
Guinea Pig ◽  
Dermal Fibroblasts ◽  
Epidermal Cells ◽  
Pig Skin ◽  
Type Iv ◽  
Basement Membrane Collagen ◽  
Membrane Collagen

Epidermal cells from adult guinea pig skin attach and differentiate preferentially on substrates of type IV (basement membrane) collagen, compared to those of types I--III collagen. In contrast, guinea pig dermal fibroblasts attach equally well to all four collagen substrates. Fibronectin mediates the attachment of fibroblasts but not of epidermal cells to collagen.

scholarly journals Subunit structure and assembly of the globular domain of basement-membrane collagen type IV

1984 ◽  
Vol 139 (2) ◽  
pp. 401-410 ◽  
Author(s):  
Sabine WEBER ◽  
Jurgen ENGEL ◽  
Hanna WIEDEMANN ◽  
Robert W. GLANVILLE ◽  
Rupert TIMPL
Keyword(s):  
Basement Membrane ◽  
Collagen Type ◽  
Collagen Type Iv ◽  
Subunit Structure ◽  
Globular Domain ◽  
Type Iv ◽  
Basement Membrane Collagen ◽  
Membrane Collagen

scholarly journals Immunohistochemical evidence for the intracellular formation of basement membrane collagen (type IV) in developing tissues.

1980 ◽  
Vol 28 (12) ◽  
pp. 1267-1274 ◽  
Author(s):  
G W Laurie ◽  
C P Leblond ◽  
I Cournil ◽  
G R Martin
Keyword(s):  
Basement Membrane ◽  
Type Iv Collagen ◽  
Early Stage ◽  
Basement Membranes ◽  
Incisor Tooth ◽  
Rat Incisor ◽  
Type Iv ◽  
Stage Of Development ◽  
Basement Membrane Collagen ◽  
Membrane Collagen

Antibodies to type IV collagen obtained from the basement membrane of the mouse EHS tumor were incubated with sections of rat incisor teeth and other tissues for immunostaining by direct or indirect methods. In all locations, the immunostaining was pronounced in basement membranes in which it was restricted to the "basal lamina" layer, from which "bridges" often extended to nearby basal laminae. Usually no immunostaining was detectable in the cells associated with the basement membranes. However, examination of the capillaries at the posterior extremity of the rat incisor tooth, where tissues are at an early stage of development, showed immunostaining not only of the basement membrane, but also of the endothelial cells. The staining was localized in rough endoplasmic reticulum cisternae, some Golgi saccules and their peripheral distensions, and structures believed to be secretory granules. These findings suggest that the synthesis of type IV collagen proceeds along the classical secretory pathways through rough endoplasmic reticulum and Golgi apparatus. At the same time, immunostaining was usually lacking in the cells of the capillaries that had migrated about 2 mm away from the posterior end of the incisor tooth and also in the cells of most other tissues examined, even though the associated basal laminae were reactive. It is, therefore, presumed that the production of type IV collagen may be high in cells at an early stage of development and that any later production and turnover of basement membrane collagen can only be minimal.

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