scholarly journals IDENTIFICATION OF GLYCOGEN IN ELECTRON MICROGRAPHS OF THIN TISSUE SECTIONS

1960 ◽  
Vol 8 (3) ◽  
pp. 575-589 ◽  
Author(s):  
Jean Paul Revel ◽  
Leonard Napolitano ◽  
Don W. Fawcett
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Animal Species ◽  
Short Exposure ◽  
Electron Microscopic ◽  
Thin Sections ◽  
Microscopic Appearance ◽  
Tissue Sections ◽  
Lead Hydroxide ◽  
Electron Microscopic Appearance

The electron microscopic appearance of glycogen has been studied in the organs of several animal species. Glycogen almost always appears as roughly circular granules from 150 to 400 A in diameter. The intrinsic electron density of glycogen varies from tissue to tissue; however, treatment with lead hydroxide as described by Watson deeply stains the granules. Glycogen pellets were isolated from some of the tissues studied by centrifugation. Such pellets were shown to be glycogen by chemical and histochemical criteria. When thin sections of the pellet are examined under the electron microscope they can be seen to consist of densely packed granules similar to those found in the intact tissues. Such pellets are also stained for electron microscopy by short exposure to lead hydroxide.

scholarly journals Light and electron microscopic demonstration of sialic acid residues with the lectin from Limax flavus: a cytochemical affinity technique with the use of fetuin-gold complexes.

1984 ◽  
Vol 32 (11) ◽  
pp. 1167-1176 ◽  
Author(s):  
J Roth ◽  
J M Lucocq ◽  
P M Charest
Keyword(s):  
Electron Microscopy ◽  
Sialic Acid ◽  
Light And Electron Microscopy ◽  
Electron Microscopic ◽  
Gold Complexes ◽  
Thin Sections ◽  
Tissue Sections ◽  
Lowicryl K4m ◽  
Hydrolysis Of ◽  
Electron Microscopic Demonstration

The development of a cytochemical affinity technique for the demonstration of sialic acid residues by light and electron microscopy is reported. The lectin from the slug Limax flavus, with its narrow specificity for N-acetyl- and N-glycolylneuraminic acid, was applied to tissue sections. Subsequently fetuin-gold complexes were used to visualize the tissue-bound lectin. Different cytochemical controls, including sugar inhibition tests, neuraminidase digestion, the use of fetuin-gold complexes alone, or acid hydrolysis of sections, proved the specificity of the technique. Postembedding staining was performed on frozen, paraffin, or semithin resin sections for light microscopy and on thin sections from low temperature Lowicryl K4M-embedded material for electron microscopy. The distribution of sialic acid residues in rat pancreas, liver, and colonic mucosa was investigated.

Electron Microscopy of Caprine Cloisonne Renal Proximal Tubular Basement Membranes

1968 ◽  
Vol 26 ◽  
pp. 192-193
Author(s):  
Norman H. Altman ◽  
I. William Grossman ◽  
Norman B. Jernigan
Keyword(s):  
Electron Microscopy ◽  
Basement Membranes ◽  
Capra Hircus ◽  
Electron Microscopic ◽  
Microscopic Appearance ◽  
Renal Biopsies ◽  
Proximal Tubular ◽  
Electron Microscopic Appearance ◽  
Renal Proximal Tubular ◽  
Metal Wires

The pigmented thickening of renal proximal convoluted tubular basement membranes in the goat (Capra hircus), resembling metal wires separating colored areas of enamelwork, has been termed Cloisonne kidney by Light. An associated hemosiderosis of the proximal tubule is occasionally present and the thickened basement membranes yield variable histochemical reactions for iron. The electron microscopy of the thickened basement membranes was studied and correlated with clinicopathological observations in an attempt to determine the pathogenesis of the Cloisonne lesion.Percutaneous renal biopsies were performed on 300 goats, and 5 of these goats were found to have the Cloisonne lesion by light microscopy. Two of the involved kidneys were examined by electron microscopy and were found to have thickened proximal convoluted tubular basement membranes of uniform electron-density approximately 3 times wider than those of a normal goat (figs. 1 and 2). One of the involved kidneys displayed electron-dense deposits measuring approximately 160 A in diameter which were aggregated into larger clumps of varying size on the outer portion of the basement membrane (fig. 2). The 160 A deposits had subunits of approximately 40 A diameter and were consistent with the electron microscopic appearance of ferritin. This particular kidney did not have an associated hemosiderosis of the proximal convoluted tubule. There was no clinicopathological evidence of hemolytic, erythrocytic or urinary abnormalities in the goats with the Cloisonne lesion when compared with a group of control goats with normal renal biopsies.

Electron microscopy of the microangiopathy and immunohistochemistry of the inflammatory infiltrate in eosinophilia-myalgia syndrome

1991 ◽  
Vol 49 ◽  
pp. 24-25
Author(s):  
Stephen A. Smith
Keyword(s):  
Electron Microscopy ◽  
B Cell ◽  
Inflammatory Infiltrate ◽  
Uranyl Acetate ◽  
T Helper ◽  
Electron Microscopic ◽  
Thin Sections ◽  
Microscopic Appearance ◽  
Muscle Fascia ◽  
Eosinophilia Myalgia Syndrome

The ingestion of L-tryptophan (LT) was linked with the eosinophilia-myalgia syndrome (EMS) in the fall of 1989. The pathology is a microangiopathy consisting of endothelial cell dysfunction and invasion of blood vessels by lymphocytes. Perimysial, fascial, and dermal perivascular lymphocytes and eosinophils are present. A contaminant in the manufacturing process of LT has been identified as the di-L-tryptophan aminal of acetaldehyde. This report describes the electron microscopic appearance of the microangiopathy and the phenotyping of the inflammatory infiltrate by immunohistochemistry.Muscle, fascia, and skin biopsies from 21 affected individuals were rapidly frozen for light microscopy, histochemistry, and immunohistochemistry. For electron microscopy tissues were preserved in 4% formaldehyde-1% glutaraldehyde with post-fixation in osmium tetroxide. Thin sections were stained with uranyl acetate and lead citrate and examined in a JEOL 100CX electron microscope. Eight anlisera were used for the immunohistochemistry to identify lymphocyte subsets and macrophages. The markers were CD-2 (pan T), CD-3 (pan T), CD-4 (T helper), CD-5 (T helper), CD-8 (T suppressor), CD-19 (B cell), CD- 22 (B cell), and CD-68 (macrophage). Three high power fields were examined from the dermis, fascia, and muscle from eight biopsies counting the immunoperoxidase labelled cells for each marker.

The Electron Microscopic Appearance of Presecreted Gastric Mucus in Cystic Fibrosis

PEDIATRICS ◽  
1974 ◽  
Vol 53 (6) ◽  
pp. 913-919
Author(s):  
Clinton B. Lillibridge ◽  
Marilyn R. Brown ◽  
Janet G. Hall
Keyword(s):  
Electron Microscopy ◽  
Cystic Fibrosis ◽  
Analysis Of Variance ◽  
Normal Subjects ◽  
Diagnostic Value ◽  
Gastric Mucus ◽  
Electron Microscopic ◽  
Microscopic Appearance ◽  
Electron Microscopic Appearance

Gastric mucosal biopsies from eight new untreated patients with cystic fibrosis (CF) and two normal subjects were studied by quantitative techniques of electron microscopy (EM) to determine whether presecreted gastric mucus was more compact (viscid) than normal. Analysis of variance techniques show no differences between means of all groups studied. These observations suggest that mucus may not be viscid prior to secretion. Electron microscopy of gastric mucus does not appear to be of diagnostic value in CF.

Electron Microscopic Appearance of Fibrin in Thin Sections

Nature ◽  
1957 ◽  
Vol 179 (4565) ◽  
pp. 868-869 ◽  
Author(s):  
W. J. S. STILL ◽  
E. H. BOULT
Keyword(s):  
Electron Microscopic ◽  
Thin Sections ◽  
Microscopic Appearance ◽  
Electron Microscopic Appearance

Edward George Gray. 11 January 1924 – 14 August 1999

2002 ◽  
Vol 48 ◽  
pp. 151-165
Author(s):  
R.W. Guillery
Keyword(s):  
Nervous System ◽  
Three Dimensional ◽  
Electron Microscopic ◽  
Early Application ◽  
Microscopic Appearance ◽  
Wide Range ◽  
Neural Tissues ◽  
Synaptic Junctions ◽  
The Central Nervous System ◽  
Electron Microscopic Appearance

George Gray was an early contributor to our knowledge of the electron microscopic appearance of the central nervous system. He was skilful with the difficult techniques for preparing the tissues, worked rapidly, and was an astute observer. Sitting with him in the dark, staring at a dim image that George was moving rapidly as he searched for significant detail, could be an exciting experience. He had clear ideas about features that mattered and could quickly relate the two-dimensional electron microscopic images to the three-dimensional neural structures under investigation. He is best known for his detailed and perceptive description of synaptic junctions in the mammalian neocortex, and his name is still linked to two distinct junctional types (Gray's type 1 and Gray's type 2), now recognized as generally distinguishing excitatory from inhibitory junctions. He studied a wide range of neural tissues, played a significant role in the early isolation of ‘synaptosomes’, contributed greatly to the rapid advance of knowledge that accompanied the early application of the electron microscope to neural tissues, and influenced a great many later fine-structural studies of the nervous system.

Bridging the Resolution Gap: Correlated 3D Light and Electron Microscopic Analysis of Large Biological Structures

1999 ◽  
Vol 5 (S2) ◽  
pp. 526-527
Author(s):  
Maryann E. Martone
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Surface Area ◽  
Light Microscope ◽  
Microscopic Analysis ◽  
Electron Microscopic Analysis ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Biological Structures ◽  
Ganglion Neurons

One class of biological structures that has always presented special difficulties to scientists interested in quantitative analysis is comprised of extended structures that possess fine structural features. Examples of these structures include neuronal spiny dendrites and organelles such as the Golgi apparatus and endoplasmic reticulum. Such structures may extend 10's or even 100's of microns, a size range best visualized with the light microscope, yet possess fine structural detail on the order of nanometers that require the electron microscope to resolve. Quantitative information, such as surface area, volume and the micro-distribution of cellular constituents, is often required for the development of accurate structural models of cells and organelle systems and for assessing and characterizing changes due to experimental manipulation. Performing estimates of such quantities from light microscopic data can result in gross inaccuracies because the contribution to total morphometries of delicate features such as membrane undulations and excrescences can be quite significant. For example, in a recent study by Shoop et al, electron microscopic analysis of cultured chick ciliary ganglion neurons showed that spiny projections from the plasmalemma that were not well resolved in the light microscope effectively doubled the surface area of these neurons.While the resolution provided by the electron microscope has yet to be matched or replaced by light microscopic methods, one drawback of electron microscopic analysis has always been the relatively small sample size and limited 3D information that can be obtained from samples prepared for conventional transmission electron microscopy. Reconstruction from serial electron micrographs has provided one way to circumvent this latter problem, but remains one of the most technically demanding skills in electron microscopy. Another approach to 3D electron microscopic imaging is high voltage electron microscopy (HVEM). The greater accelerating voltages of HVEM's allows for the use of much thicker specimens than conventional transmission electron microscopes.

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