scholarly journals Fluorescein colchicine. Synthesis, purification, and biological activity.

1978 ◽  
Vol 76 (3) ◽  
pp. 619-627 ◽  
Author(s):  
J I Clark ◽  
D Garland
Keyword(s):  
Spectral Analysis ◽  
Biological Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Fluorescein Isothiocyanate ◽  
Significant Loss ◽  
Colchicine Derivative ◽  
Layer Chromatography ◽  
Kinetics Of ◽  
Brain Tubulin

The synthesis of a fluorescent colchicine derivative permits the localization of colchicine-binding receptors in cells. Fluorescein colchicine (FC) was prepared by the addition of fluorescein isothiocyanate to deacetyl colchicine. The product, FC, was separated from the reactants by thin-layer chromatography (TLC). The purity of FC was demonstrated by TLC, UV spectral analysis, and analysis of the kinetics of photodecomposition. FC inhibited [3H] colchicine binding to purified brain tubulin. The biological activity of FC was compared to the activity of unlabeled colchicine on mitosis, motility, secretion, and myogenesis. The effects of FC were identical to those of unlabeled colchicine in all biological systems tested. The results demonstrate that FC may be substituted for colchicine in biological experiments without significant loss in specificity or effectiveness.

Characterization of fluorescein isothiocyanate dyes by infrared and thin-layer chromatography methods

1969 ◽  
Vol 29 (3) ◽  
pp. 526-532 ◽  
Author(s):  
Roger M. McKinney ◽  
Frederick C. Churchill ◽  
Janet T. Spillane ◽  
George W. Pearce
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Fluorescein Isothiocyanate ◽  
Layer Chromatography

scholarly journals Thin Layer Chromatography-A Tool to Investigate Kinetics of Michael Addition Reaction

2018 ◽  
Vol 10 (3) ◽  
pp. 323-329 ◽  
Author(s):  
M. A. B. H. Susan ◽  
G. Ara ◽  
M. M. Islam ◽  
M. M. Rahman ◽  
M. Y. A. Mollah
Keyword(s):  
Ionic Liquids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Michael Addition ◽  
Binary Systems ◽  
Addition Reaction ◽  
Average Rate ◽  
Michael Addition Reaction ◽  
Layer Chromatography ◽  
Kinetics Of

Thin layer chromatography (TLC), a tool for defining identity, purity, and quantitation of a compound, has also been proved useful for monitoring the progress of a chemical reaction. However, the   technique is yet to be exploited for investigating the kinetics of a reaction systematically. In this work, we used TLC for measuring the time for consumption of a reactant in Michael addition reaction of acetylacetone with 2-cyclohexene-1-one. Average rate of the model reaction was determined using a definite initial concentration of acetylacetone for a series of catalyst systems like NaOH, 1,8-diazabicyclo[5.4.0]undec-7-ene (DBU), DBU based protic ionic liquids (PILs) 1,8-diazabicyclo[5.4.0]undec-7-ene-8-ium hydroxide ([HDBU]OH) and 1,8-diazabicyclo[5.4.0]undec-7-ene-8-ium acetate ([HDBU]CH3COO), a DBU based aprotic ionic liquid (AIL), 8-hexyl-1,8-diazabicyclo [5.4.0]undec-7-ene-8-ium hydroxide ([C6DBU]OH), and binary systems of the ionic liquids with molecular solvents, such as, water, DBU, and acetic acid. The kinetic data on the performance of the chosen catalysts were interpreted on the basis of the established mechanism of the reaction. Finally, the prospect of the method based on TLC as a suitable technique for kinetic study of chemical reactions has been discussed.

Post-Mortem Pink Teeth

1984 ◽  
Vol 24 (2) ◽  
pp. 130-134 ◽  
Author(s):  
D. H. Clark ◽  
Marion Law
Keyword(s):  
Spectral Analysis ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Biochemical Analysis ◽  
Violent Death ◽  
Post Mortem ◽  
Pink Teeth ◽  
Layer Chromatography

One hundred coroners' cases were examined for the presence of post-mortem pink teeth. Four cases were found. The pink teeth were subjected to biochemical analysis by means of thin layer chromatography and spectral analysis in an attempt to isolate the compound causing the discoloration. The compound isolated from powdered pink dentine was found to be protoporphyrin. The reason why this phenomenon occurs in some cases and not in others suffering identical forms of violent death remains unsolved at present.

scholarly journals Evaluation of Stability of Potential Betaadrenolytics in Various Solutions by thin Layer Chromatography and by Kinetics of Hydrolysis

2012 ◽  
Vol 59 (2) ◽  
pp. 54-62
Author(s):  
M. Stankovičová ◽  
Ž. Bezáková ◽  
P. Mokrý ◽  
J. Csöllei ◽  
M. Pažitná ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Degradation Products ◽  
Base Hydrolysis ◽  
Alkaline Media ◽  
Kinetics Of Hydrolysis ◽  
Acid And Base ◽  
The Stability ◽  
Layer Chromatography ◽  
Kinetics Of

Abstract The aim of this work is the study of stability and kinetics of hydrolysis of the chosen compounds, derivatives of 2-hydroxy-3-[2-(4-methoxyphenyl)ethylamino]propyl-4- [(alkoxycarbonyl)amino]benzoates and 2-hydroxy-3-[2-(2-methoxyphenyl)ethylamino] propyl-4-[(alkoxycarbonyl)amino]benzoates with potential ultra-short beta-adrenolytic activity. The studied compounds are different in the position of the substituent on the benzene ring in the side chain as well as in the aromatic ring in position 4 with alkyl- (methyl- to butyl-) carbamate. Thin layer chromatography and UV-area spectrophotometry are used in order to establish the stability of these potential pharmaceuticals. The stability studies of the compounds were examined in acidic and alkaline media, in buffers and due oxidation at room and at elevated temperature chromatographically, and Rf values of incipient products and degradation products were detected. Kinetics of acid and base hydrolysis in various solutions at temperatures 80 °C and 100 °C were examined through UV-area spectrophotometry. Kinetic parameters such as rate constant k, half-life period t1/2 and usable life t90 were determined.

Behaviour of iodide, iodate and periodate in TLC with alcohol-aqueous ammonia eluent

1979 ◽  
Vol 44 (4) ◽  
pp. 1034-1039 ◽  
Author(s):  
Jaroslav Beneš
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Aqueous Phase ◽  
Aqueous Ammonia ◽  
Ammonium Ions ◽  
Open Chain ◽  
Alcohol Water ◽  
Layer Chromatography ◽  
Kinetics Of

The behaviour of I-, IO3- and IO4- in silica gel thin layer chromatography and their separation with ammonia-alcohol eluents (saturated open-chain C1 to C5 alcohols being used) have been studied with the help of radioisotopes. Changes in RF values of the studied anions caused by changes of the alcohol, ratio of the organic to aqueous phase in the mixture, and overall analytical concentration of ammonium ions have been determined. Influence of alcohol on kinetics of development of the chromatograms with the alcohol-water mixtures has been determined, too.

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