scholarly journals Isolation of pure cholinergic nerve endings from Torpedo electric organ. Evaluation of their metabolic properties.

1977 ◽  
Vol 75 (1) ◽  
pp. 43-55 ◽  
Author(s):  
N Morel ◽  
M Israel ◽  
R Manaranche ◽  
P Mastour-Frachon
Keyword(s):  
Synaptic Vesicles ◽  
Morphological Analysis ◽  
Electric Organ ◽  
Nerve Endings ◽  
Cholinergic Nerve ◽  
Membrane Attachment ◽  
Biochemical Measurements ◽  
Torpedo Electric Organ ◽  
Metabolic Properties

Pure cholinergic nerve endings (synaptosomes) were isolated from the electric organ of Torpedo by a rapid procedure. These synaptosomes are approximately 3 micron in diameter. They contain an occasional mitochondrion, numerous synaptic vesicles, and sometimes an active zone is observed. No postynaptic membrane attachment is found. This nerve ending fraction is extremely pure as shown by morphological controls and biochemical data. It is rich in choline acetyltransferase (450 nmol/h per mg protein) and acetylcholine (ACh) (130 nmol/mg protein). The isolated endings retain their cytoplasmic components and they synthesize ACh and are stable in vitro for several hours, as shown by biochemical measurements and morphological analysis.

Passive uptake of acetylcholine and other organic cations by synaptic vesicles from Torpedo electric organ

Biochemistry ◽  
1980 ◽  
Vol 19 (18) ◽  
pp. 4373-4379 ◽  
Author(s):  
Richard S. Carpenter ◽  
Robert Koenigsberger ◽  
Stanley M. Parsons
Keyword(s):  
Synaptic Vesicles ◽  
Electric Organ ◽  
Organic Cations ◽  
Torpedo Electric Organ

scholarly journals Stimulation of the production of unesterified fatty acids in nerve endings of guinea-pig brain in vitro by noradrenaline and 5-hydroxytryptamine

1972 ◽  
Vol 126 (3) ◽  
pp. 575-585 ◽  
Author(s):  
C. J. Price ◽  
C. E. Rowe
Keyword(s):  
Fatty Acids ◽  
Cerebral Cortex ◽  
Guinea Pig ◽  
Synaptic Vesicles ◽  
Nerve Endings ◽  
Synaptic Membranes ◽  
Pig Brain ◽  
Guinea Pig Brain ◽  
Stimulation Of

1. Noradrenaline (1mm) and 5-hydroxytryptamine (1mm) stimulated the production of unesterified palmitate, oleate, stearate and arachidonate in nerve endings (synaptosomes) isolated from combined guinea-pig cerebral cortex and cerebellum. 2. Iproniazid phosphate (0.36mm) increased the concentrations of the same acids in osmotically ruptured synaptosomes. Further addition of 1mm-noradrenaline or 1mm-5-hydroxytryptamine reversed this increase. 3. Noradrenaline (0.01mm) stimulated the production of unesterified fatty acids in isolated synaptic membranes. 5-Hydroxytryptamine (0.01mm) stimulated the production of unesterified fatty acids in synaptic membranes and synaptic vesicles.

Kinetics of cation-induced aggregation of Torpedo electric organ synaptic vesicles

1979 ◽  
Vol 557 (2) ◽  
pp. 340-353 ◽  
Author(s):  
Duncan H. Haynes ◽  
Jeffry Lansman ◽  
Anne L. Cahill ◽  
Stephen J. Morris
Keyword(s):  
Synaptic Vesicles ◽  
Electric Organ ◽  
Torpedo Electric Organ ◽  
Induced Aggregation ◽  
Kinetics Of
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