scholarly journals Electrophoretic analysis of liver and testis histones of the frog Rana pipiens.

1975 ◽  
Vol 64 (2) ◽  
pp. 389-397 ◽  
Author(s):  
D Alder ◽  
M A Gorovsky
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Rana Pipiens ◽  
Somatic Cells ◽  
Sodium Dodecyl ◽  
Electrophoretic Analysis ◽  
Polyacrylamide Gels ◽  
Histone Fraction ◽  
Calf Thymus ◽  
Late Stages ◽  
Marked Dependence

Histones were extracted from frog livers and testes and analyzed by electrophoresis on long polyacrylamide gels and on sodium dodecyl sulfate (SDS)-containing polyacrylamide gels. Frog histones were found to be similar to those of calf thymus except that frog histone fraction F2A2 showed a marked dependence on the temperature at which the long gels were run, and frog histone fraction F3 could be separated from frog F2B on SDS-containing gels. Comparisons between frog liver and frog testis histones indicated that the testis contains as its major F1 component a fast migrating species not found in liver. Testis histones also showed less microheterogeneity of fractions F3 and F2A1 than liver histones. These were the only differences observed between liver and testis histones, even when testis histones were prepared from sperm suspensions that were rich in cells in the late stages of spermiogenesis. Thus it seems that, in Rana, the electrophoretic properties of the basic proteins of sperm differ from those of somatic cells only in the nature of histone F1 and in the degree of microheterogeneity of fractions F2A1 and F3.

Electrophoretic analysis of Clostridium botulinum types A and B hemagglutinins

1980 ◽  
Vol 26 (8) ◽  
pp. 992-997 ◽  
Author(s):  
Bibhuti R. DasGupta
Keyword(s):  
Molecular Weight ◽  
Sodium Dodecyl Sulfate ◽  
Sulfate Reduction ◽  
Clostridium Botulinum ◽  
Sodium Dodecyl ◽  
Type A ◽  
Electrophoretic Analysis ◽  
The Other ◽  
Polyacrylamide Gels ◽  
Type B

The serologically related hemagglutinins of Clostridium botulinum types A and B were resolved into several bands in polyacrylamide gels in the presence of sodium dodecyl sulfate. Reduction of the samples with mercaptoethanol partly changed the band patterns, but with each hemaglutinin the two fastest moving bands in the reduced and unreduced samples were the same. In type A these two smallest units were of molecular weight 14 500 and 19 500; in type B they were of molecular weight 14 500 and 20 000. The experimentally determined molecular weight of the other resolved units agreed with those calculated on the basis that they were aggregations of the two smallest units in varying but whole multiples.

scholarly journals STUDIES ON HISTONE FRACTION F2A1 IN MACRO- AND MICRONUCLEI OF TETRAHYMENA PYRIFORMIS

1973 ◽  
Vol 57 (3) ◽  
pp. 773-781 ◽  
Author(s):  
Martin A. Gorovsky ◽  
Gloria Lorick Pleger ◽  
Josephine Bowen Keevert ◽  
Carol A. Johmann
Keyword(s):  
Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Single Species ◽  
Tetrahymena Pyriformis ◽  
Electrophoretic Analysis ◽  
Histone Fraction ◽  
Parent Molecule ◽  
Calf Thymus

Histone fraction F2A1 has been isolated and purified from macronuclei of the ciliate Tetrahymena pyriformis. It migrates as a single species on sodium dodecyl sulphate-acrylamide gel electrophoresis, with a molecular weight indistinguishable from that of calf thymus F2A1. The solubility properties of Tetrahymena F2A1 are also similar to those of calf thymus F2A1. Electrophoretic analyses on urea-acrylamide gels indicate that Tetrahymena F2A1 consists of four or five subspecies, the two fastest having electrophoretic mobilities identical with those of the two major electrophoretically separable forms of calf thymus F2A1. High resolution (long gel) electrophoresis coupled with incorporation of radioactive acetate both in vivo and in vitro suggest that, as in the case of calf thymus F2A1, differentical acetylation of a parent molecule can explain the observed electrophoretic heterogeneity of Tetrahymena F2A1. Electrophoretic analysis of histones isolated from the micronucleus, which is genetically less active than the macronucleus, indicates that it contains largely the relatively unacetylated (parent) form of histone F2A1.

Efficacy and compatibility with mass spectrometry of methods for elution of proteins from sodium dodecyl sulfate–polyacrylamide gels and polyvinyldifluoride membranes

2004 ◽  
Vol 330 (1) ◽  
pp. 87-97 ◽  
Author(s):  
Charlotte Sværke Jørgensen ◽  
Mitchell Jagd ◽  
Birgitte Kjær Sørensen ◽  
Jim McGuire ◽  
Vibeke Barkholt ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gels ◽  
Dodecyl Sulfate

scholarly journals Transformation-deficient mutants of Bacillus subtilis impaired in competence-specific nuclease activities

1982 ◽  
Vol 152 (1) ◽  
pp. 166-174
Author(s):  
J A Mulder ◽  
G Venema
Keyword(s):  
Molecular Weight ◽  
Bacillus Subtilis ◽  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Magnesium Ions ◽  
Polyacrylamide Gels ◽  
Wild Type ◽  
Molecular Weights ◽  
Defective Mutant ◽  
Mutant Strains

A comparison of the nucleolytic activities in competent and physiologically low-competent wild-type cultures of Bacillus subtilis in DNA-containing sodium dodecyl sulfate-polyacrylamide gels revealed the existence of three competence-associated nuclease activities with apparent molecular weights of 13,000, 15,000, and 26,000. The three activities, which were dependent on manganese or magnesium ions, were specifically present in the competent fraction of a competent culture. The competence-associated nucleolytic activities of eight transformation-defective mutant strains were assayed, resulting in the following three classes of mutants: (i) four strains which, according to this assay, were not impaired in any of the nucleolytic activities mentioned above; (ii) one strain which was strongly impaired in the 13,000- and 26,000-molecular-weight activities, but showed a considerable level of the 15,000-molecular-weight activity; and (iii) three strains which were severely impaired in all three activities. The results indicated that the 26,000-molecular-weight activity was a dimer of the 13,000-molecular-weight activity and that this nuclease was involved in the entry of DNA.

Detection of N-glycans on small amounts of glycoproteins in tissue samples and sodium dodecyl sulfate–polyacrylamide gels

2012 ◽  
Vol 423 (2) ◽  
pp. 253-260 ◽  
Author(s):  
Takeshi Yoshimura ◽  
Gen Yamada ◽  
Mai Narumi ◽  
Takako Koike ◽  
Akihiro Ishii ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gels ◽  
Tissue Samples ◽  
Dodecyl Sulfate
Sign in / Sign up

Export Citation Format

Share Document