scholarly journals Calcium-containing structures in vertebrate glial cells. Ultrastructural and microprobe analysis.

1975 ◽  
Vol 64 (2) ◽  
pp. 322-330 ◽  
Author(s):  
P Gambetti ◽  
S E Erulkar ◽  
A P Somlyo ◽  
N K Gonatas
Keyword(s):  
Glial Cells ◽  
Electron Probe Microanalysis ◽  
Electron Probe ◽  
Microprobe Analysis ◽  
Dense Material ◽  
Electron Dense Material ◽  
Human Astrocytes ◽  
Calcium And Phosphorus ◽  
Osmiophilic Particles

Electron probe microanalysis has revealed that vesicular or cisternal structures containing electron-dense material in frog ependymal glial cells contain deposits of calcium and phosphorus. The so-called "osmiophilic particles" in human astrocytes also contain calcium. It is suggested that these organelles are storage sites of calcium.

Origine et formation de différents types de vacuoles induites par la multiplication d'Alphavirus Sindbis dans divers systemes cellulaires

1979 ◽  
Vol 25 (12) ◽  
pp. 1452-1459 ◽  
Author(s):  
Yves Lombard ◽  
Philippe Poindron ◽  
Aimé Porte
Keyword(s):  
Endoplasmic Reticulum ◽  
Glial Cells ◽  
Chicken Embryo ◽  
Cytoplasmic Membrane ◽  
Dense Material ◽  
Newborn Mice ◽  
Electron Dense Material ◽  
Double Membrane ◽  
Single Membrane ◽  
Chicken Embryo Fibroblasts

Spherule-containing vacuoles and nucleocapsid-bearing vacuoles (cytopathic vacuoles types 1 and 2 respectively of Grimley et al. 1968) induced by Alphavirus Sindbis were studied in brains from newborn mice, chicken embryo fibroblasts, and two lines of tumoral glial cells from muridae. Endoplasmic reticulum (ER) elements and finely granular electron-dense material also seen in contact with nucleocapsids seemed to be involved in the formation of the classical single-membrane spherule-containing vacuoles. A second type of spherule-containing vacuoles were characterized by their double membrane and an amorphous electron-dense content and were probably derived from mitochondria. Nucleocapsid-bearing vacuoles were formed from modified ER elements and seemed to be linked to excessive synthesis of viral material. Such ER alterations were not observed in RG6 cells. In these cells, there were only spherule-containing vacuoles, while nucleocapsids were seen associated with the cytoplasmic membrane only.

Electron Probe Microanalysis: A Review

1968 ◽  
Vol 22 (5) ◽  
pp. 395-403 ◽  
Author(s):  
Kurt F. J. Heinrich
Keyword(s):  
Quantitative Analysis ◽  
Future Development ◽  
Electron Microprobe ◽  
Electron Probe Microanalysis ◽  
Electron Probe ◽  
Microprobe Analysis ◽  
Electron Microprobe Analysis ◽  
The Future

This review covers the development of electron microprobe analysis in the last ten years. After a brief decription of the instrument, the following subjects are discussed: progress and instrumentation; progress in the theory of quantitative analysis; and the future development of microprobe analysis.

Electron Microprobe Analysis of Precipitates in the Laser Crystal Material Fluorapatite

1969 ◽  
Vol 23 (5) ◽  
pp. 490-492 ◽  
Author(s):  
P. J. Walitsky ◽  
R. H. Hopkins
Keyword(s):  
Electron Microprobe ◽  
Electron Probe Microanalysis ◽  
Electron Probe ◽  
Microprobe Analysis ◽  
Electron Microprobe Analysis ◽  
Laser Crystal ◽  
Crystal Material ◽  
Melt Composition

Electron probe microanalysis has been used to identify precipitates in calcium fluorapatite Ca5(PO4)3F. Crystals grown from nonstoichiometric melts were found to have defects of either CaF2 or Ca3(PO4)2 depending on the melt composition.

Electron Probe Microanalysis of Frozen Hydrated Kidneys, Isolated Cells, and Cultured Cells

1982 ◽  
Vol 40 ◽  
pp. 402-403 ◽  
Author(s):  
Claude Lechene
Keyword(s):  
Liquid Nitrogen ◽  
Electron Probe Microanalysis ◽  
Electron Probe ◽  
Cultured Cells ◽  
Liquid Nitrogen Temperature ◽  
Elemental Content ◽  
Isolated Cells ◽  
Renal Tubular Cells ◽  
Diamond Saw ◽  
Saw Blade

Electron probe microanalysis of frozen hydrated kidneysThe goal of the method is to measure on the same preparation the chemical elemental content of the renal luminal tubular fluid and of the surrounding renal tubular cells. The following method has been developed. Rat kidneys are quenched in solid nitrogen. They are trimmed under liquid nitrogen and mounted in a copper holder using a conductive medium. Under liquid nitrogen, a flat surface is exposed by sawing with a diamond saw blade at constant speed and constant pressure using a custom-built cryosaw. Transfer into the electron probe column (Cameca, MBX) is made using a simple transfer device maintaining the sample under liquid nitrogen in an interlock chamber mounted on the electron probe column. After the liquid nitrogen is evaporated by creating a vacuum, the sample is pushed into the special stage of the instrument. The sample is maintained at close to liquid nitrogen temperature by circulation of liquid nitrogen in the special stage.

Comparative Morphology of Intercellular Bridges Between Developing Germ Cells of the Ovary

1970 ◽  
Vol 28 ◽  
pp. 328-329
Author(s):  
J. R. Ruby ◽  
R. F. Dyer ◽  
R. G. Skalko ◽  
R. F. Gasser ◽  
E. P. Volpe
Keyword(s):  
Germ Cells ◽  
Rana Pipiens ◽  
Comparative Morphology ◽  
Dense Material ◽  
Individual Species ◽  
Microscope Examination ◽  
Electron Dense Material ◽  
Intercellular Bridges ◽  
Cytoplasmic Side ◽  
Intercellular Connections

An electron microscope examination of fetal ovaries has revealed that developing germ cells are connected by intercellular bridges. In this investigation several species have been studied including human, mouse, chicken, and tadpole (Rana pipiens). These studies demonstrate that intercellular connections are similar in morphology regardless of the species.Basically, all bridges are characterized by a band of electron-dense material on the cytoplasmic side of the tri-laminar membrane surrounding the connection (Fig.l). This membrane is continuous with the plasma membrane of the conjoined cells. The dense material, however, never extends beyond the limits of the bridge. Variations in the configuration of intercellular connections were noted in all ovaries studied. However, the bridges in each individual species usually exhibits one structural characteristic seldom found in the others. For example, bridges in the human ovary very often have large blebs projecting from the lateral borders whereas the sides of the connections in the mouse gonad merely demonstrate a slight convexity.

Analysis of completed commercial semiconductors using EPMA

1996 ◽  
Vol 54 ◽  
pp. 502-503
Author(s):  
R. Packwood ◽  
M.W. Phaneuf ◽  
V. Weatherall ◽  
I. Bassignana
Keyword(s):  
Electron Probe Microanalysis ◽  
Electron Probe ◽  
Semiconductor Industry ◽  
Detection Limits ◽  
High Technology ◽  
Depth Resolution ◽  
Analytical Instruments ◽  
Wide Range ◽  
Numerous Element ◽  
Choice Method

The development of specialized analytical instruments such as the SIMS, XPS, ISS etc., all with truly incredible abilities in certain areas, has given rise to the notion that electron probe microanalysis (EPMA) is an old fashioned and rather inadequate technique, and one that is of little or no use in such high technology fields as the semiconductor industry. Whilst it is true that the microprobe does not possess parts-per-billion sensitivity (ppb) or monolayer depth resolution it is also true that many times these extremes of performance are not essential and that a few tens of parts-per-million (ppm) and a few tens of nanometers depth resolution is all that is required. In fact, the microprobe may well be the second choice method for a wide range of analytical problems and even the method of choice for a few.The literature is replete with remarks that suggest the writer is confusing an SEM-EDXS combination with an instrument such as the Cameca SX-50. Even where this confusion does not exist, the literature discusses microprobe detection limits that are seldom stated to be as low as 100 ppm, whereas there are numerous element combinations for which 10-20 ppm is routinely attainable.

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