scholarly journals CHARACTERIZATION OF THE EXTERNAL PROTEINS OF HAMSTER FIBROBLASTS

1974 ◽  
Vol 62 (2) ◽  
pp. 438-448 ◽  
Author(s):  
Richard O. Hynes ◽  
Kenneth C. Humphryes
Keyword(s):  
Hyaluronic Acid ◽  
Cell Lines ◽  
Fibroblast Cell ◽  
Transformed Cells ◽  
Metabolic Labeling ◽  
Polyacrylamide Gels ◽  
Molecular Weights ◽  
Fibroblast Cell Lines

The results of metabolic labeling studies and enzymatic treatments followed by analysis on polyacrylamide gels show that the external proteins of hamster fibroblast cell lines, which have been identified by lactoperoxidase-catalyzed iodination, do not contain sulphated mucopolysaccharides or hyaluronic acid and are probably unrelated to collagen. Several of the iodinated species comigrate with carbohydrate-containing molecules. In particular, the major iodine-labeled polypeptide of normal fibroblasts appears to be a glycoprotein. This glycoprotein is absent or much reduced in virus-transformed cells, as detected both by iodination and by metabolic labeling. We conclude that the major iodinated polypeptide is not detected on transformed cells because it is absent rather than because it is masked. Approximate molecular weights of the external proteins are also reported.

scholarly journals Establishment and characterization of equine fibroblast cell lines transformed in vivo and in vitro by BPV-1: Model systems for equine sarcoids

Virology ◽  
2008 ◽  
Vol 373 (2) ◽  
pp. 352-361 ◽  
Author(s):  
Z.Q. Yuan ◽  
E.A. Gault ◽  
P. Gobeil ◽  
C. Nixon ◽  
M.S. Campo ◽  
...  
Keyword(s):  
Cell Lines ◽  
Fibroblast Cell ◽  
Model Systems ◽  
Fibroblast Cell Lines

Establishment and characterization of fibroblast cell lines from the skin of the Yangtze finless porpoise

2011 ◽  
Vol 47 (9) ◽  
pp. 618-630 ◽  
Author(s):  
Jingzhen Wang ◽  
Weiting Su ◽  
Wenhui Nie ◽  
Jinhuan Wang ◽  
Wuhan Xiao ◽  
...  
Keyword(s):  
Cell Lines ◽  
Fibroblast Cell ◽  
Finless Porpoise ◽  
Yangtze Finless Porpoise ◽  
Fibroblast Cell Lines

Characterization of the action of retinoids on mouse fibroblast cell lines

1979 ◽  
Vol 119 (2) ◽  
pp. 289-299 ◽  
Author(s):  
A.M. Jetten ◽  
M.E.R. Jetten ◽  
S.S. Shapiro ◽  
J.P. Poon
Keyword(s):  
Cell Lines ◽  
Fibroblast Cell ◽  
Mouse Fibroblast ◽  
Mouse Fibroblast Cell ◽  
Fibroblast Cell Lines

scholarly journals Establishment and characterization of immortalized Gli-null mouse embryonic fibroblast cell lines

2008 ◽  
Vol 9 (1) ◽  
pp. 49 ◽  
Author(s):  
Robert J Lipinski ◽  
Maarten F Bijlsma ◽  
Jerry J Gipp ◽  
David J Podhaizer ◽  
Wade Bushman
Keyword(s):  
Cell Lines ◽  
Fibroblast Cell ◽  
Mouse Embryonic Fibroblast ◽  
Null Mouse ◽  
Mouse Embryonic Fibroblast Cell ◽  
Embryonic Fibroblast ◽  
Fibroblast Cell Lines

scholarly journals Immunization with SV40-transformed cells yields mainly MHC-restricted monoclonal antibodies.

1986 ◽  
Vol 164 (1) ◽  
pp. 196-210 ◽  
Author(s):  
B G Froscher ◽  
N R Klinman
Keyword(s):  
B Cells ◽  
Cell Surface ◽  
Cell Lines ◽  
Fibroblast Cell ◽  
Surface Antigens ◽  
Transformed Cells ◽  
Cell Surface Antigens ◽  
Class I Mhc ◽  
Sv40 Transformation ◽  
Fibroblast Cell Lines

Recognition of antigens on cell surfaces only in the context of the MHC-encoded alloantigens of the presenting cell (self + X) has classically been considered the province of T cells. However, evidence from several sources has indicated that B cells and antibodies can exhibit self + X-restricted recognition as well. This report concerns the mAb response to SV40-transformed H-2b fibroblast cell lines. The specificities of the antibodies obtained have been analyzed for binding to a panel of SV40-transformed H-2-syngeneic, H-2-allogeneic, and H-2b mutant fibroblast cell lines, as well as cell lines not bearing cell surface SV40 transformation-associated antigens. A large proportion of primary C57BL/6 (71%) and BALB/c (68%) splenic B cells responding to in vitro stimulation with SV40-transformed H-2b cells recognize cell surface antigens associated with SV40 transformation only when coexpressed with MHC antigens of the immunizing cell, particularly the Kb molecule, on transformed cells. To extensively define the nature of antigen recognition by these antibodies, we have generated and characterized nine hybridoma antibodies specific for SV40-transformed H-2-syngeneic cell lines. Seven of these hybridoma antibodies recognize SV40-associated transformation antigens in the context of H-2b molecules. Six of these are restricted by the Kb molecule and discriminate among a panel of SV40-transformed Kb mutant cell lines, thus confirming the participation of class I MHC-encoded molecules in the recognition by B cells of cell surface antigens.

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