scholarly journals Long-Term Cultivation of Human Cells (Chang) in Chemically Defined Medium and Effect of Added Peptone Fractions

1959 ◽  
Vol 6 (3) ◽  
pp. 535-536 ◽  
Author(s):  
Richard Holmes
Keyword(s):  
Defined Medium ◽  
Human Cells ◽  
Chemically Defined Medium

scholarly journals An inverse small molecule screen to design a chemically defined medium supporting long-term growth of Drosophila cell lines

2014 ◽  
Vol 10 (10) ◽  
pp. 2713-2723 ◽  
Author(s):  
M. Burnette ◽  
T. Brito-Robinson ◽  
J. Li ◽  
J. Zartman
Keyword(s):  
Protein Interaction ◽  
Defined Medium ◽  
Chemically Defined Medium ◽  
Drosophila Cell ◽  
Interaction Database ◽  
Novel Approach ◽  
Small Molecule Screen ◽  
Growth Promoting ◽  
Protein Interaction Database

We describe a novel approach to screen for growth promoting compounds and score putative targets using a drug–protein interaction database.

scholarly journals SERUM FRACTIONATION AND THE EFFECTS OF BOVINE SERUM FRACTIONS ON HUMAN CELLS GROWN IN A CHEMICALLY DEFINED MEDIUM

1961 ◽  
Vol 10 (3) ◽  
pp. 389-401 ◽  
Author(s):  
Richard Holmes ◽  
Sonya W. Wolfe
Keyword(s):  
Sodium Bicarbonate ◽  
Hela Cells ◽  
Bovine Serum ◽  
Defined Medium ◽  
Human Cells ◽  
Cell Multiplication ◽  
Chemically Defined Medium ◽  
Continuous Growth ◽  
Serum Fractions ◽  
Serum Fractionation

Serum has been fractionated by curtain electrophoresis using carboxymethyl cellulose dissolved in sodium bicarbonate electrolyte. Various fractions were produced from bovine serum and added to replicate cultures of Chang's endoepithelial cells and HeLa cells grown in a chemically defined medium. The effects of each of the various fractions on the appearance of the cultures and on cell multiplication were studied. Three different fractions were obtained and two were subjected to further purification. One fraction associated with albumin promoted survival, attachment, and flattening as well as cell multiplication. A second fraction associated with the alpha globulins promoted survival and multiplication of some cells. A third fraction caused cells to aggregate and form free floating clumps. An adequate chemically defined medium for continuous growth of human cells was used throughout the study. The response of cells to alterations in their environment which simulated some of the effects produced by serum fractions is described.

scholarly journals Long-term, hormone-responsive organoid cultures of human endometrium in a chemically defined medium

2017 ◽  
Vol 19 (5) ◽  
pp. 568-577 ◽  
Author(s):  
Margherita Y. Turco ◽  
Lucy Gardner ◽  
Jasmine Hughes ◽  
Tereza Cindrova-Davies ◽  
Maria J. Gomez ◽  
...  
Keyword(s):  
Defined Medium ◽  
Human Endometrium ◽  
Chemically Defined Medium ◽  
Organoid Cultures

Long-term cultivation of cryopreserved human fetal brain cells in a chemically defined medium

1989 ◽  
Vol 30 (2) ◽  
pp. 121-131 ◽  
Author(s):  
G. Haselbacher ◽  
P. Groscurth ◽  
U. Otten ◽  
H. Vedder ◽  
U. Lutz ◽  
...  
Keyword(s):  
Fetal Brain ◽  
Defined Medium ◽  
Brain Cells ◽  
Chemically Defined Medium ◽  
Human Fetal Brain
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