scholarly journals A Neoplastic Connective Tissue Mast Cell Capable of Continuous Growth In Tissue Culture

1959 ◽  
Vol 6 (3) ◽  
pp. 361-368 ◽  
Author(s):  
William J. Williams ◽  
Edna Larson ◽  
Theodore L. Phillips
Keyword(s):  
Tissue Culture ◽  
Mast Cell ◽  
Connective Tissue ◽  
Periodic Acid ◽  
Calf Serum ◽  
Basal Medium ◽  
Tissue Mast Cell ◽  
Periodic Acid Schiff ◽  
Continuous Growth ◽  
Sudan Black B

A neoplastic connective tissue mast cell from a dog mast cell sarcoma has been grown in tissue culture for 50 passages over a period of 2 years. The cells were grown as monolayer cultures in glass bottles, using Eagle's basal medium fortified with calf serum. The cultures were contaminated with an Alkaligenes sp. for 10 months but finally were sterilized bacteriologically by treatment with specific antiserum combined with antibiotics. The cells grow in a fibroblastic pattern, and contain mitochondria, mast cell granules, and lipid granules or droplets. The mast cell granules stain basophilic with Giemsa's stain and metachromatically with azure A or toluidine blue. They also stain with Sudan black B and with periodic acid-Schiff stain. The interphase nuclei are vesicular, contain from 1 to 20 nucleoli, and frequently show bizarre outlines. Multinucleate cells are often seen, as are mitotic figures. Extracellular fibrous material occurs in all cultures and apparently originates from the cell surface. This material does not have the structure of connective tissue fibers and has not been identified. The cells develop an increased number of metachromatic granules when grown in medium containing heparin and an increased number of sudanophilic granules when grown in medium containing stearic acid. Only small amounts of histamine were present in the tumor from which this cell line was derived and in the cells grown in tissue culture.

scholarly journals EXTRACTION RESISTANCE OF SUDAN STAINED MAST CELLS AFTER PREVIOUS ACID TREATMENT

1964 ◽  
Vol 12 (7) ◽  
pp. 530-532 ◽  
Author(s):  
JOHN R. FEAGLER ◽  
J. F. A. MCMANUS
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Chemical Property ◽  
Cell Nucleus ◽  
Acid Treatment ◽  
Periodic Acid ◽  
Hydrogen Ion ◽  
Periodic Acid Schiff ◽  
Sudan Black B ◽  
Sudan Dye

1. Sudan black B alone stains the mast cells very faintly. The granules do not appear very distinct or numerous. 2. The periodic acid Schiff (PAS) technique stains the mast cells; however, the results are variable. When stained, the mast cell granules appear slightly darker than the cytoplasm. 3. When PAS and Sudan black are used together, the mast cell granules gain an avidity for the dye and are stained black. 4. Brief acid treatment prior to immersion in Sudan black results in granules gaining an avidity for the Sudan dye that is extraction resistant. The reaction appears dependent upon modifying the mast cell granules by acid treatment. It appears as though the hydrogen ion changes some physical or chemical property of the granules so that they gain an avidity for the dye. 5. Acetylated Sudan black shows an affinity for the mast cell granules greater than Sudan black alone. This is not increased by previous acid treatment. Acetylated Sudan black also stains the mast cell nucleus.

scholarly journals Studies of connective tissue mast cell-mediated cytotoxicity

1989 ◽  
Vol 93 (3) ◽  
pp. 423-428 ◽  
Author(s):  
Michael D. Tharp ◽  
Candace Kasper ◽  
Dwain Thiele ◽  
Michael R. Charley ◽  
Donald A. Kennerly ◽  
...  
Keyword(s):  
Mast Cell ◽  
Connective Tissue ◽  
Tissue Mast Cell

HISTOLOGICAL EFFECTS OF 3-TRIFLUORMETHYL-4-NITROPHENOL (TFM) ON LARVAL LAMPREY AND TROUT

1963 ◽  
Vol 41 (1) ◽  
pp. 51-61 ◽  
Author(s):  
Ronald M. Christie ◽  
Helen I. Battle
Keyword(s):  
Connective Tissue ◽  
Prolonged Exposure ◽  
Periodic Acid ◽  
Secretory Activity ◽  
The Body ◽  
Salmo Gairdneri ◽  
Periodic Acid Schiff ◽  
Sinusoidal Dilatation ◽  
Gill Filaments ◽  
Red Coloration

Larvae of the lamprey, Entosphenus lamottei (Le Sueur), and rainbow trout, Salmo gairdneri Richardson, were exposed to the sodium salt of 3-trifluormethyl-4-nitrophenol (TFM) in concentrations of 0.75 p.p.m., 3.00 p.p.m., and 6.00 p.p.m. Microscopic examination of changes induced in the gills, liver, cloacal region, and musculature were made on 7-micron sections stained with Harris' haematoxylin and Bowie's eosin, and in the gill region with periodic acid Schiff reagent. A comparison of the degree of the effects in the two species was made by planimetry of the vascular, cellular, and edematous areas from enlarged drawings of sections.Upon exposure to lethal concentrations of TFM, the body of the larval lamprey becomes distended at the pharyngeal level and heavy cords of mucus emerge from the external gill clefts. A deep red coloration is evident in the pharyngeal region consequent upon vasodilatation of the arterioles and capillaries of the gill filaments. Trout exhibit a similar vasodilatation of the gills together with increased mucous secretion. Edema in the connective tissue between the respiratory epithelium and the vascular endothelium is induced in both species. After prolonged exposure to TFM, the mucous cells in the lining of the branchial chamber and covering the tips of the gill filaments are actively discharging their secretions or completely spent.Certain effects induced by TFM in the larval lamprey are not evident in the trout. The cloacal region takes on a deep red coloration due to dilatation of the venous sinuses and the liver becomes reddish because of sinusoidal dilatation. Extensive edema of the fibrous connective tissue of the skeletal musculature is characteristically present. A slightly increased secretory activity of mucous-secreting cells may occur in the epidermis.With the techniques employed in this study, there was no evidence in either species of cytological or histological changes in the nervous tissue, cardiac musculature, notochord, alimentary canal (including the haemopoietic typhlosole of the lamprey), or mesonephros.

scholarly journals Genome-Wide Transcriptional Profiling Reveals Connective Tissue Mast Cell Accumulation in Bronchopulmonary Dysplasia

2012 ◽  
Vol 186 (4) ◽  
pp. 349-358 ◽  
Author(s):  
Soumyaroop Bhattacharya ◽  
Diana Go ◽  
Daria L. Krenitsky ◽  
Heidi L. Huyck ◽  
Siva Kumar Solleti ◽  
...  
Keyword(s):  
Mast Cell ◽  
Connective Tissue ◽  
Bronchopulmonary Dysplasia ◽  
Transcriptional Profiling ◽  
Tissue Mast Cell ◽  
Genome Wide ◽  
Cell Accumulation

Extracellular Localization of Human Connective Tissue Mast Cell Granule Contents

1990 ◽  
Vol 96 (6) ◽  
pp. 857-863 ◽  
Author(s):  
Michael S. Kaminer ◽  
Robert M. Lavker ◽  
Laurence J. Walsh ◽  
Diana. Whitaker ◽  
Burton. Zweiman
Keyword(s):  
Mast Cell ◽  
Connective Tissue ◽  
Cell Granule ◽  
Tissue Mast Cell ◽  
Mast Cell Granule

scholarly journals Acute lymphoblastic leukemia with unusual cytoplasmic granulation: a morphologic, cytochemical, and ultrastructural study

Blood ◽  
1986 ◽  
Vol 68 (2) ◽  
pp. 406-411 ◽  
Author(s):  
J Fradera ◽  
E Velez-Garcia ◽  
JG White
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Lymphoblastic Leukemia ◽  
Periodic Acid ◽  
Periodic Acid Schiff ◽  
Acute Leukemias ◽  
Ultrastructural Studies ◽  
Sudan Black B ◽  
Blast Cells ◽  
Chediak Higashi Syndrome ◽  
Schiff Reaction

Abstract The classification of the acute leukemias depends mainly on the morphologic and cytochemical evaluation of the blast forms. One of the main accepted morphologic criteria in the differentiation between acute lymphoblastic leukemia (ALL) and acute myeloblastic leukemia (AML) is the absence of granules in the blast cells of ALL. We evaluated a patient with ALL in whom granules were present in the cytoplasm of 35% of the blast cells, as seen in AML. Cytochemical evaluation was performed, including periodic acid-Schiff reaction, Sudan black B, alpha-naphthyl acetate, alpha-naphthyl butyrate, naphthol AS-D chloroacetate, and acid phosphatase stains. The results of these studies confirmed the morphologic impression and diagnosis of ALL. Ultrastructural evaluation revealed that the granules consisted of many tiny vesicles closely packed together in a proteinaceous matrix, resembling to some extent the inclusions described in lymphocytes in the Chediak-Higashi syndrome, but clearly different. The morphologic, cytochemical, and ultrastructural studies of this unique case are presented in detail. To our knowledge, this is the first time that such granules have been described in blast cells of ALL.

scholarly journals A VERTICAL MICROSYSTEM FOR DISCONTINUOUS ELECTROPHORESIS OF INSECT TISSUE PROTEINS USING THIN SHEETS OF POLYACRYLAMIDE GEL

1972 ◽  
Vol 20 (5) ◽  
pp. 368-384 ◽  
Author(s):  
ANITA C. BEEN ◽  
ELLEN M. RASCH
Keyword(s):  
Periodic Acid ◽  
Polyacrylamide Gel ◽  
Thin Sheets ◽  
Periodic Acid Schiff ◽  
Sudan Black B ◽  
Tissue Extracts ◽  
Salivary Gland Cells ◽  
Schiff Reaction ◽  
Coomassie Brilliant ◽  
Nonspecific Esterases

Proteins extracted from individual pairs of salivary glands or other larval tissues of Sciara coprophila (Diptera) were separated in a vertical microsystem for discontinuous electrophoresis using thin sheets of polyacrylamide gel cast in multiple layers of varying pore size. After electrophoresis at 150 volts for 40 min, gels were stained ( a) for total proteins with Coomassie brilliant blue, ( b) for glycoproteins with the periodic acid-Schiff reaction, ( c) for lipoproteins with Sudan black B or ( d) for nonspecific esterases with fast blue RR as coupler and α-naphthol acetate as substrate. Sequential application of these reactions to individual gel sectors permitted direct comparisons of protein profiles for 15-20 different samples of tissue extracts carried on a single gel sheet in adjacent lanes and thus subjected to identical conditions of electrophoresis. Representative photographs and densitometric scans are presented to show the suitability of thin gel sheets for autoradiography and for both qualitative and quantitative evaluation of tissue-specific differences in patterns of protein banding found for salivary gland cells, the gastric ceca, or the hemolymph of individual Sciara larvae sacrificed at particular stages of fourth instar development. Innovative details of methodology are presented, including the use of a microspectrophotometer to scan electropherograms of insect proteins and several types of human blood serum.

scholarly journals IMMUNOLOGIC STUDIES OF HEART TISSUE

1961 ◽  
Vol 113 (1) ◽  
pp. 1-16 ◽  
Author(s):  
Melvin H. Kaplan ◽  
Frederick D. Dallenbach
Keyword(s):  
Heart Disease ◽  
Connective Tissue ◽  
Gamma Globulin ◽  
Fluorescent Antibody ◽  
Periodic Acid ◽  
Heart Tissue ◽  
Periodic Acid Schiff ◽  
Schiff Reagent ◽  
Points Of View ◽  
Rheumatic Heart

Using fluorescent antibody methods, deposits of bound gamma globulin, as determined in unfixed washed sections of auricular appendages from rheumatic hearts, were noted in a significant number (18 per cent) of 100 specimens studied. Such deposits were observed in myofibers, sarcolemma, interstitial connective tissue, and vessel walls. Albumin and fibrin were generally found absent from these sites. Control hearts from normal and pathologic material, including postmortem and biopsied specimens, in general, did not reveal such deposits. These various tissue sites which contained bound gamma globulin frequently exhibited evidence of alteration as indicated both by enhanced affinity for eosin and by strongly positive reaction with the periodic acid-Schiff reagent, and appeared comparable in some cases to "fibrinoid." Bound gamma globulin was not observed in cellular or stromal components of Aschoff lesions, nor was the occurrence of Aschoff lesions correlated with presence of bound gamma globulin. It is suggested that deposition of gamma globulin and the eosinophilic alteration associated with such deposition are related to certain of the pathologic changes of rheumatic heart disease. The nature of such deposits of gamma globulin was considered from immune and non-immune points of view.

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