scholarly journals ISOLATION AND CHARACTERIZATION OF MITOCHONDRIAL DNA FROM DROSOPHILA MELANOGASTER

1973 ◽  
Vol 56 (2) ◽  
pp. 580-589 ◽  
Author(s):  
Mary Lake Polan ◽  
Susan Friedman ◽  
Joseph G. Gall ◽  
Walter Gehring
Keyword(s):  
Mitochondrial Dna ◽  
Drosophila Melanogaster ◽  
Average Length ◽  
Buoyant Density ◽  
Main Peak ◽  
Thermal Transitions ◽  
Isolation And Characterization ◽  
Circular Molecule ◽  
Isopycnic Centrifugation

Mitochondrial DNA (MtDNA) with a neutral buoyant density of 1.681 g/cm3 has been isolated from unfertilized eggs of Drosophila melanogaster. This DNA is a circular molecule with an average length of 5.3 µm; it reassociates with a low C0t1/2 after denaturation, and in alkaline isopycnic centrifugation it separates into strands differing in density by 0.005 g/cm3. MtDNA isolated from purified mitochondria of unfertilized eggs or from total larval DNA melts with three distinct thermal transitions. The three melting temperature values suggest that the molecule may have three regions differing in average base composition. DNA isolated from unfertilized eggs of D. melanogaster contains approximately equal amounts of MtDNA and another DNA with a buoyant density of 1.697 g/cm3, slightly less dense than main peak DNA. The possibility that the heavier DNA fraction consists of amplified ribosomal DNA was excluded by hybridization experiments, but otherwise nothing is known of its origin or function.

scholarly journals CHARACTERIZATION OF THE DNA IN DROSOPHILA MELANOGASTER

Genetics ◽  
1972 ◽  
Vol 72 (3) ◽  
pp. 419-430
Author(s):  
E C Travaglini ◽  
J Petrovic ◽  
J Schultz
Keyword(s):  
Drosophila Melanogaster ◽  
Embryonic Development ◽  
Buoyant Density ◽  
Concomitant Increase ◽  
Satellite Dnas ◽  
Total Dna ◽  
Isopycnic Centrifugation

ABSTRACT DNA has been quantitatively extracted from Drosophila melanogaster at various stages of embryonic development and analyzed by isopycnic centrifugation in CsCl and by fractionation on methylated albumin columns. The DNA is composed of three main classes of DNA, as defined by their buoyant density, ρ, in CsCl: a bulk DNA, ρ = 1.699 g cm-3, and two satellite DNAs, ρ = 1.685 g cm-3 and ρ = 1.669 g cm-3. These three types of DNA persist throughout the development of the insect. In the unfertilized egg, 80% of the total DNA consists of the satellite DNAs; this amount decreases to 18% during the first three hours after fertilization and then remains constant through embryogenesis. There is a concomitant increase of the satellite DNA's with the bulk DNA after blastoderm formation.

scholarly journals Isolation and characterization of the dopa decarboxylase gene of Drosophila melanogaster

1981 ◽  
Vol 1 (6) ◽  
pp. 475-485
Author(s):  
J Hirsh ◽  
N Davidson
Keyword(s):  
Drosophila Melanogaster ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Isolation Procedure ◽  
Chromosome Band ◽  
Dopa Decarboxylase ◽  
Developmental Profile ◽  
Isolation And Characterization

We have isolated chromosomal deoxyribonucleic acid clones containing the Drosophila dopa decarboxylase gene. We describe an isolation procedure which can be applied to other nonabundantly expressed Drosophila genes. The dopa decarboxylase gene lies within or very near polytene chromosome band 37C1-2. The gene is interrupted by at least one intron, and the primary mode of regulation is pretranslational. At least two additional sequences hybridized by in vivo ribonucleic acid-derived probes are found within a 35-kilobase region surrounding the gene. The developmental profile of ribonucleic acid transcribed from one of these regions differs from that of the dopa decarboxylase transcript.

Isolation and characterization of a mitochondrial RNA polymerase from Drosophila melanogaster

1987 ◽  
Vol 65 (2) ◽  
pp. 173-182 ◽  
Author(s):  
Michael Goldenthal ◽  
James T. Nishiura
Keyword(s):  
Drosophila Melanogaster ◽  
Rna Polymerase ◽  
Nuclear Dna ◽  
Gradient Centrifugation ◽  
Mitochondrial Rna ◽  
Mitochondrial Rna Polymerase ◽  
Isolation And Characterization ◽  
Cscl Gradient ◽  
Nuclear Rna

A DNA-dependent RNA polymerase was solubilized from sucrose gradient isolated, DNase-treated mitochrondria of Drosophila melanogaster. The isolated mitochondria were not detectably contaminated with nuclear DNA as shown by CsCl gradient centrifugation and polylysine Kieselguhr chromatography. The detergent-solubilized RNA polymerase was sensitive to rifampicin, resistant to α-amanitin, had an apparent molecular mass of about 60 kilodaltons, and displayed a tendency to aggregate, both in crude extracts or when purified. The mitochondrial RNA polymerase could be distinguished from nuclear RNA polymerases on the basis of size, salt optima, rifampicin sensitivity, and α-amanitin resistance.

Isolation and characterization of rat liver mitochondrial DNA

1970 ◽  
Vol 48 (3) ◽  
pp. 455-468 ◽  
Author(s):  
A.T. Leffler ◽  
E. Creskoff ◽  
S.W. Luborsky ◽  
V. McFarland ◽  
P.T. Mora
Keyword(s):  
Mitochondrial Dna ◽  
Rat Liver ◽  
Isolation And Characterization
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