scholarly journals EFFECTS OF α-AMANITIN ON IN VITRO LABELING OF RNA FROM DEFINED NUCLEAR COMPONENTS IN SALIVARY GLAND CELLS FROM CHIRONOMUS TENTANS

1972 ◽  
Vol 53 (2) ◽  
pp. 523-531 ◽  
Author(s):  
E. Egyházi ◽  
B. D'Monte ◽  
J.-E. Edström
Keyword(s):  
Salivary Gland ◽  
Normal Band ◽  
Chironomus Tentans ◽  
Gland Cells ◽  
Large Size ◽  
Rna Labeling ◽  
Salivary Gland Cells ◽  
Rna Formation ◽  
18 S Ribosomal Rna

The effect of α-amanitin on nucleoside labeling of RNA in nucleoli, chromosomes, nuclear sap, and cytoplasm from Chironomus tentans salivary gland cells was investigated by radioautography and gel electrophoresis. Preribosomal RNA formation and processing in the nucleolus was not measurably influenced by the drug, and both 28 S and 18 S ribosomal RNA were transferred to the cytoplasm. In the chromosomes the heterogeneous RNA labeling was completely inhibited for the large size range (above 45–50 S) and partially for the low range. The labeling of 4–5 S chromosomal RNA was only moderately reduced. Most of the chromosomes showed radioautographically a disappearance of the normal band pattern, but some retained a pattern of weakly labeled bands. The electrophoretic results for the nuclear sap paralleled those for the chromosomes. The effect of α-amanitin on RNA labeling in these cells is similar but not identical to that of the substituted benzimidazole 5,6-dichloro-1(ß-D-ribofuranosyl) benzimidazole (DRB).

scholarly journals NUCLEOLAR-DERIVED RIBONUCLEIC ACID IN CHROMOSOMES, NUCLEAR SAP, AND CYTOPLASM OF CHIRONOMUS TENTANS SALIVARY GLAND CELLS

1971 ◽  
Vol 51 (2) ◽  
pp. 355-368 ◽  
Author(s):  
U. Ringborg ◽  
L. Rydlander
Keyword(s):  
Molecular Weight ◽  
Salivary Gland ◽  
High Molecular Weight ◽  
Ribosomal Rna ◽  
Chironomus Tentans ◽  
Gland Cells ◽  
Composite Gels ◽  
Salivary Gland Cells

The distribution of monodisperse high molecular weight RNA (38, 30, 28, 23, and 18S RNA) was studied in the salivary gland cells of Chironomus tentans. RNA labeled in vitro and in vivo with tritiated cytidine and uridine was isolated from microdissected nucleoli, chromosomes, nuclear sap, and cytoplasm and analyzed by electrophoresis on agarose-acrylamide composite gels. As shown earlier, the nucleoli contain labeled 38, 30, and 23S RNA. In the chromosomes, labeled 18S RNA was found in addition to the 30 and 23S RNA previously reported. The nuclear sap contains labeled 30 and 18S RNA, and the cytoplasm labeled 28 and 18S RNA. On the basis of the present and earlier analyses, it was concluded that the chromosomal monodisperse high molecular weight RNA fractions (a) show a genuine chromosomal localization and are not due to unspecific contamination, (b) are not artefacts caused by in vitro conditions, but are present also in vivo, and (c) are very likely related to nucleolar and cytoplasmic (pre)ribosomal RNA. The 30 and 23S RNA components are likely to be precursors to 28 and 18S ribosomal RNA. The order of appearance of the monodisperse high molecular weight RNA fractions in the nucleus is in turn and order: (a) nucleolus, (b) chromosomes, and (c) nuclear sap. Since both 23 and 18S RNA are present in the chromosomes, the conversion to 18S RNA may take place there. On the other hand, 30S RNA is only found in the nucleus while 28S RNA can only be detected in the cytoplasm, suggesting that this conversion takes place in connection with the exit of the molecule from the nucleus.

Microinjection of anti-topoisomerase I immunoglobulin G into nuclei of Chironomus tentans salivary gland cells leads to blockage of transcription elongation

1987 ◽  
Vol 7 (12) ◽  
pp. 4308-4316
Author(s):  
E Egyházi ◽  
E Durban
Keyword(s):  
Salivary Gland ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Immunoglobulin G ◽  
Topoisomerase I ◽  
Chironomus Tentans ◽  
Balbiani Ring ◽  
Gland Cells ◽  
Salivary Gland Cells ◽  
Transcriptional Process

Purified anti-topoisomerase I immunoglobulin G (IgG) was microinjected into nuclei of Chironomus tentans salivary gland cells, and the effect on DNA transcription was investigated. Synthesis of nucleolar preribosomal 38S RNA by RNA polymerase I and of chromosomal Balbiani ring RNA by RNA polymerase II was inhibited by about 80%. The inhibitory action of anti-topoisomerase I IgG could be reversed by the addition of exogenous topoisomerase I. Anti-topoisomerase I IgG had less effect on RNA polymerase II-promoted activity of other less efficiently transcribing heterogeneous nuclear RNA genes. The pattern of inhibition of growing nascent Balbiani ring chains indicated that the transcriptional process was interrupted at the level of chain elongation. The highly decondensed state of active Balbiani ring chromatin, however, remained unaffected after injection of topoisomerase I antibodies. These data are consistent with the interpretation that topoisomerase I is an essential component in the transcriptional process but not in the maintenance of the decondensed state of active chromatin.

scholarly journals Anti-Ro and anti-La autoantibodies induce TNF-α production by human salivary gland cells: an in vitro study

Reumatismo ◽  
2011 ◽  
Vol 59 (3) ◽  
Author(s):  
M. Sisto ◽  
S. Lisi ◽  
M. D'Amore ◽  
V. Mitolo ◽  
P. Scagliusi
Keyword(s):  
Salivary Gland ◽  
In Vitro Study ◽  
Vitro Study ◽  
Human Salivary Gland ◽  
Gland Cells ◽  
Salivary Gland Cells ◽  
Tnf Α

scholarly journals BK virus has tropism for human salivary gland cells in vitro: Implications for transmission

Virology ◽  
2009 ◽  
Vol 394 (2) ◽  
pp. 183-193 ◽  
Author(s):  
Liesl K. Jeffers ◽  
Vicki Madden ◽  
Jennifer Webster-Cyriaque
Keyword(s):  
Salivary Gland ◽  
Bk Virus ◽  
Human Salivary Gland ◽  
Gland Cells ◽  
Salivary Gland Cells

Electrophoretic characterization of nucleolar RNA from Chironomus tentans salivary gland cells

1970 ◽  
Vol 51 (2) ◽  
pp. 327-340 ◽  
Author(s):  
U. Ringborg ◽  
B. Daneholt ◽  
J.-E. Edström ◽  
E. Egyházi ◽  
B. Lambert
Keyword(s):  
Salivary Gland ◽  
Chironomus Tentans ◽  
Gland Cells ◽  
Salivary Gland Cells ◽  
Nucleolar Rna

In vitro and in vivo imaging of intracellular Ca2+ responses in salivary gland cells

2015 ◽  
Vol 57 (2) ◽  
pp. 69-75 ◽  
Author(s):  
Akihiro Nezu ◽  
Takao Morita ◽  
Akihiko Tanimura
Keyword(s):  
Salivary Gland ◽  
In Vivo Imaging ◽  
Gland Cells ◽  
Salivary Gland Cells
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