scholarly journals ERYTHROPOIETIN EFFECTS ON FETAL MOUSE ERYTHROID CELLS

1971 ◽  
Vol 51 (3) ◽  
pp. 585-595 ◽  
Author(s):  
David H. K. Chui ◽  
Meir Djaldetti ◽  
Paul A. Marks ◽  
Richard A. Rifkind
Keyword(s):  
Protein Synthesis ◽  
Adult Mouse ◽  
Absolute Number ◽  
Precursor Cells ◽  
Erythroid Precursor ◽  
Hemoglobin Synthesis ◽  
Fetal Mouse ◽  
Globin Chains ◽  
Principal Effect ◽  
Stimulation Of

The effect of the hormone, erythropoietin, on cultures of erythroblasts derived from the livers of fetal C57BL/6J mice was examined. An increase both in the content and in the rate of synthesis of normal adult mouse globin chains was detected in hormone-treated cultures. The rate of protein synthesis by individual erythroblasts does not increase in response to the hormone, whereas the absolute number of hemoglobin-synthesizing cells does increase and accounts for the observed stimulation of hemoglobin synthesis. The principal effect of erythropoietin appears to be upon the population of immature erythroid precursor cells which persists in the presence of the hormone, the cells maintaining their ability to replicate, and their capacity to differentiate into hemoglobinizing erythroblasts. In the absence of hormone, already committed erythroblasts continue their development, but erythropoiesis is not sustained.

scholarly journals In vitro stimulation of primate hemoglobin synthesis by L-thyroxine

Blood ◽  
1977 ◽  
Vol 49 (3) ◽  
pp. 407-413 ◽  
Author(s):  
JE Fuhr ◽  
N Gengozian ◽  
M Overton
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Leucine Incorporation ◽  
Hemoglobin Synthesis ◽  
Globin Chains ◽  
Stimulation Of ◽  
Marrow Cells

Abstract Bone marrow cells from adult and abortus primates (marmosets) were incubated in vitro to determine their responsiveness to L-thyroxine. 3H- leucine incorporation into purified globin chains was the parameter assayed to determine responsiveness. Bone marrow from spontaneously aborted animals consistently was stimulated by the presence of physiologic levels of L-thyroxine. Bone marrow cells from adult animals were unaffected by the hormone.

scholarly journals Developmental potential of the earliest precursor cells from the adult mouse thymus.

1991 ◽  
Vol 174 (6) ◽  
pp. 1617-1627 ◽  
Author(s):  
L Wu ◽  
M Antica ◽  
G R Johnson ◽  
R Scollay ◽  
K Shortman
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Adult Mouse ◽  
Precursor Cells ◽  
Lymphoid Cells ◽  
Agar Culture ◽  
Homogeneous Population ◽  
Erythroid Precursor ◽  
Developmental Potential ◽  
Mouse Thymus

A new, numerically minute population of cells representing the earliest T precursor cells in the adult mouse thymus has recently been isolated. This population has been shown to be similar to bone marrow hemopoietic stem cells in surface antigenic phenotype and to express moderate levels of CD4. We now show, by fluorescence-activated cell sorting and intrathymic transfer to irradiated mice, that this apparently homogeneous population differs from multipotent stem cells in expressing the surface stem cell antigen 2 (Sca-2), that it differs from most early B lineage cells in lacking B220 and class II major histocompatibility complex expression, and that it binds rhodamine 123 like an activated rather than a quiescent cell. Irradiated recipient mice differing at the Ly 5 locus were used to compare the developmental potential of these early intrathymic precursors with bone marrow stem cells. Only T lineage product cells were detected when the intrathymic precursor population was transferred back into an irradiated thymus. However, when the intrathymic precursor population was transferred intravenously, it displayed the capacity to develop into both B and T lymphoid cells in recipient bone marrow, spleen, and lymph nodes, but no donor-derived myeloid cells were detected. The absence of myeloid and erythroid precursor activity was confirmed by showing that the intrathymic precursor population was unable to develop into myeloid or erythroid spleen colonies on intravenous transfer or to form colonies in an agar culture. These findings indicate that this earliest intrathymic precursor population has become restricted (or strongly biased) to lymphoid lineage development, but not exclusively to T lymphocytes.

scholarly journals In vitro stimulation of primate hemoglobin synthesis by L-thyroxine

Blood ◽  
1977 ◽  
Vol 49 (3) ◽  
pp. 407-413
Author(s):  
JE Fuhr ◽  
N Gengozian ◽  
M Overton
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Leucine Incorporation ◽  
Hemoglobin Synthesis ◽  
Globin Chains ◽  
Stimulation Of ◽  
Marrow Cells

Bone marrow cells from adult and abortus primates (marmosets) were incubated in vitro to determine their responsiveness to L-thyroxine. 3H- leucine incorporation into purified globin chains was the parameter assayed to determine responsiveness. Bone marrow from spontaneously aborted animals consistently was stimulated by the presence of physiologic levels of L-thyroxine. Bone marrow cells from adult animals were unaffected by the hormone.

scholarly journals Stimulation of cAMP signalling allows isolation of clonal pancreatic precursor cells from adult mouse pancreas

Diabetologia ◽  
2006 ◽  
Vol 49 (10) ◽  
pp. 2359-2367 ◽  
Author(s):  
T. Yamamoto ◽  
E. Yamato ◽  
H. Taniguchi ◽  
M. Shimoda ◽  
F. Tashiro ◽  
...  
Keyword(s):  
Adult Mouse ◽  
Precursor Cells ◽  
Mouse Pancreas ◽  
Camp Signalling ◽  
Stimulation Of

EFFECT OF ACTINOMYCIN D ON TSH-INDUCED STIMULATION OF THYROIDAL PROTEIN SYNTHESIS IN THE RAT

1974 ◽  
Vol 77 (1) ◽  
pp. 64-70 ◽  
Author(s):  
Gustav Wägar
Keyword(s):  
Protein Synthesis ◽  
Actinomycin D ◽  
The Other ◽  
Leucine Incorporation ◽  
Short Term ◽  
Other Hand ◽  
Thyroid Glands ◽  
Translational Level ◽  
Stimulation Of

ABSTRACT Whether the short-term regulation of thyroidal protein synthesis by TSH occurs at the transcriptional or the translational level was tested by measuring the effect of actinomycin D (act D) on the TSH-induced stimulation of L-14C-leucine incorporation into the thyroidal proteins of rats. TSH was injected 6 h before the rats were killed. The thyroid glands were then removed and incubated in vitro in the presence of L-14C-leucine for 2 h. The pronounced stimulation of leucine incorporation in the TSH-treated animals was depressed as compared with controls but still significant even when the animals had been pre-treated with 100 μg act D 24 and 7 h before sacrifice. On the other hand, act D strongly decreased incorporation of 3H-uridine into RNA. Short-term regulation of thyroidal protein synthesis by TSH appears to be partly but not wholly dependent on neosynthesis of RNA. Hence regulation may partly occur at the translation level of protein synthesis.

scholarly journals Isolation of erythropoietin-sensitive cells from Friend virus-infected marrow cultures: characteristics of the erythropoietin response

Blood ◽  
1983 ◽  
Vol 61 (4) ◽  
pp. 751-758 ◽  
Author(s):  
M Bondurant ◽  
M Koury ◽  
SB Krantz ◽  
T Blevins ◽  
DT Duncan
Keyword(s):  
Terminal Differentiation ◽  
Erythroid Differentiation ◽  
Precursor Cells ◽  
Erythroid Cells ◽  
Friend Virus ◽  
Isolated Cells ◽  
Two Dimensional Gel Electrophoresis ◽  
Erythroid Precursor ◽  
Short Time

Abstract Murine erythroid precursor cells, stimulated to proliferate in vitro in the absence of added erythropoietin (EP) by the anemia strain of Friend virus (FVA), will subsequently respond to EP by complete erythrocyte differentiation. If not exposed to EP, the erythroid cells divide for about 120 hr in culture, and they maintain the potential for full differentiation in response to EP added at any time during the period from 72 to 120 hr. Between 96 and 120 hr of culture without added EP, the EP-sensitive erythroid precursor cells that have formed discrete erythroid bursts can be isolated in relatively large numbers from such cultures by plucking with a Pasteur pipette. The addition of EP initiates the final stages of erythroid differentiation, including heme synthesis in 70%-80% of these isolated cells. With respect to homogeneity of the precursor cells, quantity of EP-responsive cells obtainable, and uniformity of EP responsiveness, this system is uniquely favorable for biochemical studies of the late differentiation effects of EP. The overall changes in gene expression accompanying EP- induced terminal differentiation were examined by two-dimensional gel electrophoresis of proteins labeled for a short time with radioactive amino acids. Several new proteins are synthesized in these erythroid cells during terminal differentiation, but the number is a very small percentage of the total number of proteins being made. Thus, in this system, the effect of EP is to initiate expression of a small group of genes, including those for globins, spectrin, and other proteins involved in the final stages of erythroid differentiation.

Sign in / Sign up

Export Citation Format

Share Document