scholarly journals DEOXYRIBONUCLEIC ACID SYNTHESIS AND MITOSIS IN DIFFERENTIATED CARDIAC MUSCLE CELLS OF CHICK EMBRYOS

1970 ◽  
Vol 47 (1) ◽  
pp. 310-316 ◽  
Author(s):  
Roslyn B. Weinstein ◽  
Elizabeth D. Hay
1960 ◽  
Vol 8 (2) ◽  
pp. 333-343 ◽  
Author(s):  
Irwin R. Konigsberg ◽  
Norma McElvain ◽  
Martha Tootle ◽  
Heinz Herrmann

The effects of a nitrogen mustard on both the morphology and several synthetic capacities of embryonic chick skeletal muscle cells in monolayer culture have been examined. Concentrations of nitrogen mustard which profoundly inhibit deoxyribonucleic acid synthesis specifically do not inhibit the development of multinuclearity in the contractile "ribbons" which form rapidly in culture. Nitrogen mustard affects the nuclear morphology of "fibroblast-like" and multinuclear muscle cells differentially. The mononucleated cells in treated cultures exhibit extreme nuclear enlargement which distinguishes them from the multinuclear cells as well as from both types of cells in control cultures. The nuclei of the multinuclear cells which form after nitrogen mustard treatment, however, do give evidence of having been affected by the treatment. They exhibit somewhat less uniformity of size than similar cells in control cultures. Analogous differences were described by Bodenstein (3) between potentially proliferating cells and postmitotic differentiating cells, marked nuclear enlargement being characteristic of cells in the proliferative zone. The results are more compatible with the hypothesis that multinuclearity arises through successive cell fusion than through amitotic nuclear multiplication, since it is unlikely that any form of nuclear replication could occur in the absence of DNA synthesis.


1975 ◽  
Vol 152 (2) ◽  
pp. 421-423 ◽  
Author(s):  
Roland Roguet ◽  
Roland Bourrillon

Concanavalin A and Robinia pseudoacacia lectin decreased [3H]thymidine incorporation into acid-insoluble material of fibroblasts cultured from 6–10-day chick embryos. In contrast, these lectins stimulated [3H]thymidine incorporation in cells from 16-day embryos. These effects are due to neither [3H]thymidine permeability modification nor toxicity of the lectins. The specificity of lectin action was proved by blocking experiments with α-methyl mannopyranoside and with anti-(Robinia lectin) serum.


1938 ◽  
Vol 70 (4) ◽  
pp. 441-449 ◽  
Author(s):  
George S. de Rényi ◽  
Mary Jane Hogue

2004 ◽  
Vol 29 (1) ◽  
pp. 83-95 ◽  
Author(s):  
Hung-Chien Wu ◽  
Jaung-Geng Lin ◽  
Chun-Hsien Chu ◽  
Yung-Hsien Chang ◽  
Chung-Gwo Chang ◽  
...  

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