scholarly journals 3H LABELING PATTERNS OF PERMANENT CELL LINE CHROMOSOMES SHOWING PULVERIZATION OR ACCENTUATED SECONDARY CONSTRICTIONS

1969 ◽  
Vol 40 (2) ◽  
pp. 553-561 ◽  
Author(s):  
Charles P. Miles ◽  
Frank O'Neill
Keyword(s):  
Cell Line ◽  
Permanent Cell Line ◽  
Permanent Cell ◽  
Secondary Constrictions

Cytogenetic characterization of a new human papillary thyroid carcinoma permanent cell line (GLAG-66)

1993 ◽  
Vol 67 (2) ◽  
pp. 117-122 ◽  
Author(s):  
P. Antonini ◽  
G. Linares ◽  
N. Gaillard ◽  
A.M. Venuat ◽  
M. Schlumberger ◽  
...  
Keyword(s):  
Papillary Thyroid Carcinoma ◽  
Thyroid Carcinoma ◽  
Cell Line ◽  
Papillary Thyroid ◽  
Permanent Cell Line ◽  
Permanent Cell ◽  
Cytogenetic Characterization

In vitro model of angiogenesis using a human endothelium-derived permanent cell line: Contributions of induced gene expression, G-proteins, and integrins

1992 ◽  
Vol 153 (3) ◽  
pp. 437-449 ◽  
Author(s):  
Jeffrey Bauer ◽  
Michael Margolis ◽  
Clara Schreiner ◽  
Cora-Jean Edgell ◽  
Jane Azizkhan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
G Proteins ◽  
In Vitro Model ◽  
Permanent Cell Line ◽  
Permanent Cell ◽  
Vitro Model ◽  
Human Endothelium

Establishment and characterization of a new permanent cell line (GDM-1) from a patient with myelomonoblastic leukemia

1982 ◽  
Vol 6 (6) ◽  
pp. 743-752 ◽  
Author(s):  
Hannah Ben-Bassat ◽  
Aviva Korkesh ◽  
Ruth Voss ◽  
Rachel Leizerowitz ◽  
Aaron Polliack
Keyword(s):  
Cell Line ◽  
Permanent Cell Line ◽  
Permanent Cell

Induction of a step in carcinogenesis that is normally associated with mutagenesis by nonmutagenic concentrations of 5-azacytidine

1984 ◽  
Vol 4 (7) ◽  
pp. 1231-1237
Author(s):  
N Bouck ◽  
D Kokkinakis ◽  
J Ostrowsky
Keyword(s):  
Cell Line ◽  
Cell Fusion ◽  
Somatic Mutation ◽  
Complementation Group ◽  
Single Step ◽  
Permanent Cell Line ◽  
Bhk Cells ◽  
Permanent Cell ◽  
A Cell

The permanent cell line BHK-21/cl 13 can be transformed by mutagenic carcinogens as the result of the induction of a recessive somatic mutation. Yet when these cells were treated with 5-azacytidine under conditions in which no mutants resistant to either ouabain or 6-thioguanine could be detected, they were transformed efficiently. These transformants were induced, not selected. 6-Azacytidine was ineffective at transforming BHK cells; 2'-deoxy-5-azacytidine was exceptionally effective. When tested by cell fusion, transformants induced by 5-azacytidine fell into the same complementation group as those induced by highly mutagenic carcinogens, but they were phenotypically distinct in that they were unstable during prolonged passage and rarely displayed the temperature-limited phenotypes so common among BHK transformants induced by strongly mutagenic carcinogens. These results raise the possibility that a cell can be induced by either genetic or epigenetic means to traverse the same single step in carcinogenesis.

C-type particles produced by a permanent cell line from a leukemic pig

Virology ◽  
1974 ◽  
Vol 57 (1) ◽  
pp. 179-188 ◽  
Author(s):  
Volker Moennig ◽  
Hermann Frank ◽  
Gerhard Hunsmann ◽  
Peter Ohms ◽  
Heinz Schwarz ◽  
...  
Keyword(s):  
Cell Line ◽  
Permanent Cell Line ◽  
Permanent Cell

Electrogenic sodium absorption and chloride secretion by an inner medullary collecting duct cell line (mIMCD-K2)

1995 ◽  
Vol 268 (2) ◽  
pp. F347-F355 ◽  
Author(s):  
N. L. Kizer ◽  
B. Lewis ◽  
B. A. Stanton
Keyword(s):  
Cell Line ◽  
Collecting Duct ◽  
T Antigen ◽  
Sodium Absorption ◽  
Sv40 Large T Antigen ◽  
Permanent Cell Line ◽  
Inner Medullary Collecting Duct ◽  
Permanent Cell ◽  
Medullary Collecting Duct

The initial segment of the inner medullary collecting duct (IMCDi) absorbs Na+ by an electrogenic mechanism and plays an important role in regulating the composition and volume of the urine. The purpose of the present study was to establish a permanent cell line derived from the IMCDi, which has the ion transport properties of the IMCDi in vivo. To this end, we isolated IMCD cells from the IMCDi of a mouse, Tg(SV40E) Bri 7, transgenic for the early region of SV40 (large T antigen) and established a permanent cell line, mIMCD-K2, by clonal dilution. mIMCD-K2 cells retain many differentiated characteristics of the IMCDi, including amiloride-sensitive electrogenic Na+ absorption stimulated by nanomolar concentrations of aldosterone. Aldosterone (1.5 x 10(-6) M) increased Na+ absorption from 0.2 +/- 0.1 to 4.6 +/- 1.7 microA/cm2. In addition, the cells secrete Cl- by an electrogenic mechanism at a rate of 0.5 +/- 0.1 microA/cm2. We propose that IMCDi cells either absorb or secrete NaCl depending on NaCl homeostasis. The mIMCD-K2 cell line should be useful for studying the cellular mechanisms responsible for electrogenic Na+ and Cl- transport in the IMCDi.

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