scholarly journals The Effect of HCl Hydrolysis on the Retention of Thymidine in DNA

1958 ◽  
Vol 4 (6) ◽  
pp. 701-706 ◽  
Author(s):  
R. E. Savage ◽  
Walter Plaut
Keyword(s):  
Hydrochloric Acid ◽  
Significant Loss ◽  
Optimal Time ◽  
Feulgen Staining ◽  
Hydrolysis Time

Allium roots grown in C14-thymidine and H3-thymidine media were treated with N hydrochloric acid at 60°C. as in standard Feulgen hydrolysis. The retention of the radioactive thymidine in DNA as a function of hydrolysis time was studied autoradiographically. No significant loss of label was detected until hydrolysis was extended beyond the optimal time for Feulgen staining. The data are consistent with the assumption that there is no significant loss of DNA during normal Feulgen hydrolysis in the material used.

scholarly journals Study on Aqueous Enzymatic Extraction Process of Common Pistache Oil

2021 ◽  
Vol 251 ◽  
pp. 02058
Author(s):  
Feng Xuehua ◽  
Tao Ali ◽  
Song Zurong ◽  
Gong Panpan
Keyword(s):  
Ph Value ◽  
Orthogonal Experiment ◽  
Extraction Process ◽  
Optimal Time ◽  
Process Conditions ◽  
Optimal Process ◽  
Enzymatic Extraction ◽  
Hydrolysis Time ◽  
Aqueous Enzymatic Extraction ◽  
The Common

The aqueous enzymatic method was applied to extract the common pistache oil and the optimal extraction process conditions were identified. By observing the effect of enzymatic hydrolysis time, pH value, temperature on aqueous enzymatic extraction process and performing the orthogonal experiment based on the single factor test, the optimal process parameters were obtained, namely, the optimal time, temperature, and pH value were respectively 3 h, 50℃, and 7 with a final extraction rate of 25.38 %.

scholarly journals Influence of Hydrolysis Conditions on Characteristics of Nanocrystalline Cellulose Extracted from Ramie Fibers by Hydrochloric Acid Hydrolysis

2020 ◽  
Author(s):  
Kusmono Kusmono ◽  
Dimas Abdillah Akbar
Keyword(s):  
Thermal Stability ◽  
Hydrochloric Acid ◽  
Acid Hydrolysis ◽  
Acid Concentration ◽  
Crystallinity Index ◽  
Hydrochloric Acid Concentration ◽  
Hydrolysis Time ◽  
Study Results ◽  
Ramie Fibers ◽  
Hydrolysis Conditions

Abstract Nanocrystalline celluloses (NCCs) were successfully extracted from ramie fibers using chemical pretreatments followed by hydrochloric acid hydrolysis. The effects of acid concentration and hydrolysis time on the characteristics of NCCs were investigated in this study. Results showed that the optimal hydrolysis conditions were found to be 6 M hydrochloric acid concentration at 45 °C for 70 min. The obtained NCC had a rod like-shape with an average of 8.07 nm in diameter, 158.51 nm in length, 22.37 in aspect ratio, 89.61% in the crystallinity index, and 5.81 nm in crystallite size. The higher crystallinity and thermal stability were exhibited by NCCs compared to both raw fibers and chemically purified cellulose. The hydrolysis time had a significant effect on crystallinity and thermal stability. The crystallinity index and thermal stability of NCCs were obtained to decrease with increasing hydrolysis time.

scholarly journals Pembuatan Bioetanol Dari Pati Umbi Talas (Colocasia esculenta [L] Schott) Melalui Hidrolisis Asam dan Fermentasi

2017 ◽  
Vol 5 (2) ◽  
pp. 79
Author(s):  
Mariskian M. Sadimo ◽  
Irwan Said ◽  
Kasmudin Mustapa
Keyword(s):  
Hydrochloric Acid ◽  
Room Temperature ◽  
Sugar Content ◽  
Total Sugar ◽  
Raw Material ◽  
Hydrolysis Time ◽  
Total Sugar Content ◽  
High Sugar ◽  
High Sugar Content ◽  
Root Starch

Taro plant contains high enough of carbohydrate, so it can be used as an alternative raw material for producing bioethanol. This study aimed to determine the ratio of hydrochloric acid to taro root starch and hydrolysis time of taro root starch for producing a high sugar content, as well as to determine the bioethanol content produced from the fermentation of taro root starch using baker’s yeast. The results showed that the best ratio of hydrochloric acid 15% to the taro root starch was at 10:1 (v/w), resulted in a total sugar content of 0.651%. The best hydrolysis time of taro root starch was 2.5 hours, resulted in sugar content of 0.653%. The fermentation of sugar resulted in from hydrolysis was carried out at room temperature for 5 days. The ethanol content obtained from the fermentation was 7.716%.

Feulgen Staining of Rabbit Spermatozoa: Hydrolysis Time and Distribution of Chromatin

1965 ◽  
Vol 40 (4) ◽  
pp. 193-197 ◽  
Author(s):  
Roger Bouter ◽  
G. W. Salisbury ◽  
F. N. Baker
Keyword(s):  
Feulgen Staining ◽  
Hydrolysis Time ◽  
Rabbit Spermatozoa

scholarly journals Disease Risk Forecasting with Bayesian Learning Networks: Application to Grape Powdery Mildew (Erysiphe necator) in Vineyards

Agronomy ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 622
Author(s):  
Weixun Lu ◽  
Nathaniel K. Newlands ◽  
Odile Carisse ◽  
David E. Atkinson ◽  
Alex J. Cannon
Keyword(s):  
Powdery Mildew ◽  
Bayesian Learning ◽  
Disease Risk ◽  
Significant Loss ◽  
Optimal Time ◽  
Learning Networks ◽  
Model Learning ◽  
Erysiphe Necator ◽  
Complex Interactions ◽  
Susceptibility Factors

Powdery mildew (Erysiphe necator) is a fungal disease causing significant loss of grape yield in commercial vineyards. The rate of development of this disease varies annually and is driven by complex interactions between the pathogen, its host, and environmental conditions. The long term impacts of weather and climate variability on disease development is not well understood, making the development of efficient and durable strategies for disease management challenging, especially under northern conditions. We present a probabilistic, Bayesian learning network model to explore the complex causal interactions between environment, pathogen, and host for three different susceptible northern grape cultivars in Quebec, Canada. This approach combines environmental (weather, climate), pathogen (development stages), and host (crop cultivar-specific susceptibility) factors. The model is evaluated in an operational forecast mode with supervised and algorithm model learning and integrating Global Forecast System (GFS) Ensemble Reforecasts (GEFSR). A model-guided fungicide spray strategy is validated for guiding spray decisions up to 6 days with a 10-day forecast of potential spray efficacy under rain washed off conditions. The model-guided strategy improves fungicide spray decisions; decreasing the number of sprays, and identifying the optimal time to spray to increase spray effectiveness.

A New AID for Interpolating and Assessing Collision Strengths and Rate Coefficients

1988 ◽  
Vol 102 ◽  
pp. 107-110
Author(s):  
A. Burgess ◽  
H.E. Mason ◽  
J.A. Tully
Keyword(s):  
Significant Loss ◽  
Impact Excitation ◽  
Rate Coefficients ◽  
Electron Impact Excitation ◽  
Graphical Display ◽  
Practical Procedure ◽  
Positive Ions ◽  
Allowed Transition ◽  
Computational Errors ◽  
Existing Data

AbstractA new way of critically assessing and compacting data for electron impact excitation of positive ions is proposed. This method allows one (i) to detect possible printing and computational errors in the published tables, (ii) to interpolate and extrapolate the existing data as a function of energy or temperature, and (iii) to simplify considerably the storage and transfer of data without significant loss of information. Theoretical or experimental collision strengths Ω(E) are scaled and then plotted as functions of the colliding electron energy, the entire range of which is conveniently mapped onto the interval (0,1). For a given transition the scaled Ω can be accurately represented - usually to within a fraction of a percent - by a 5 point least squares spline. Further details are given in (2). Similar techniques enable thermally averaged collision strengths upsilon (T) to be obtained at arbitrary temperatures in the interval 0 < T < ∞. Application of the method is possible by means of an interactive program with graphical display (2). To illustrate this practical procedure we use the program to treat Ω for the optically allowed transition 2s → 2p in ArXVI.

Fine Structure of Platelet Interaction with a New Microcrystalline Collagen Preparation

1974 ◽  
Vol 32 ◽  
pp. 58-59
Author(s):  
W. H. Zucker ◽  
R. G. Mason
Keyword(s):  
Fine Structure ◽  
Platelet Aggregation ◽  
Hydrochloric Acid ◽  
Whole Blood ◽  
Platelet Adhesion ◽  
Hemostatic Agent ◽  
Native Collagen ◽  
Fibrillar Morphology ◽  
Clinical Interest ◽  
New Form

Platelet adhesion initiates platelet aggregation and is an important component of the hemostatic process. Since the development of a new form of collagen as a topical hemostatic agent is of both basic and clinical interest, an ultrastructural and hematologic study of the interaction of platelets with the microcrystalline collagen preparation was undertaken.In this study, whole blood anticoagulated with EDTA was used in order to inhibit aggregation and permit study of platelet adhesion to collagen as an isolated event. The microcrystalline collagen was prepared from bovine dermal corium; milling was with sharp blades. The preparation consists of partial hydrochloric acid amine collagen salts and retains much of the fibrillar morphology of native collagen.

Effects of sterilization modes on the mechanical strengths of plaster of paris implants

1989 ◽  
Vol 47 ◽  
pp. 890-891
Author(s):  
K. Cowden ◽  
B. Giammara ◽  
T. Devine ◽  
J. Hanker
Keyword(s):  
Gamma Radiation ◽  
Calcium Sulfate ◽  
Significant Loss ◽  
Potassium Sulfate ◽  
Limiting Factors ◽  
Radiation Sterilization ◽  
Plaster Of Paris ◽  
Hardness Tester ◽  
Dry Heat ◽  
Calcium Sulfate Hemihydrate

Plaster of Paris (calcium sulfate hemihydrate, CaSO4. ½ H2O) has been used as a biomedical implant material since 1892. One of the primary limiting factors of these implants is their mechanical properties. These materials have low compressive and tensile strengths when compared to normal bone. These are important limiting factors where large biomechanical forces exist. Previous work has suggested that sterilization techniques could affect the implant’s strength. A study of plaster of Paris implant mechanical and physical properties to find optimum sterilization techniques therefore, could lead to a significant increase in their application and promise for future use as hard tissue prosthetic materials.USG Medical Grade Calcium Sulfate Hemihydrate Types A, A-1 and B, were sterilized by dry heat and by gamma radiation. Types A and B were additionally sterilized with and without the setting agent potassium sulfate (K2SO4). The plaster mixtures were then moistened with a minimum amount of water and formed into disks (.339 in. diameter x .053 in. deep) in polyethylene molds with a microspatula. After drying, the disks were fractured with a Stokes Hardness Tester. The compressive strengths of the disks were obtained directly from the hardness tester. Values for the maximum tensile strengths σo were then calculated: where (P = applied compression, D = disk diameter, and t = disk thickness). Plaster disks (types A and B) that contained no setting agent showed a significant loss in strength with either dry heat or gamma radiation sterilization. Those that contained potassium sulfate (K2SO4) did not show a significant loss in strength with either sterilization technique. In all comparisons (with and without K2SO4 and with either dry heat or gamma radiation sterilization) the type B plaster had higher compressive and tensile strengths than that of the type A plaster. The type A-1 plaster however, which is specially modified for accelerated setting, was comparable to that of type B with K2SO4 in both compressive and tensile strength (Table 1).

Bidirectional Associations Between Self-Perceptions of Aging and Processing Speed Across 3 Years

GeroPsych ◽  
2017 ◽  
Vol 30 (2) ◽  
pp. 49-59 ◽  
Author(s):  
Anna L. Seidler ◽  
Julia K. Wolff
Keyword(s):  
Processing Speed ◽  
Personal Growth ◽  
Significant Loss ◽  
Path Model ◽  
Outcome Variable ◽  
Beneficial Effects ◽  
Self Perceptions ◽  
Chi Squared ◽  
Perceptions Of Aging ◽  
Bidirectional Associations

Abstract. Background: Previous studies point to a potential relationship between self-perceptions of aging (SPA) and cognitive performance. However, most of these studies are limited by their experimental design. Previous longitudinal studies looked solely at memory as an outcome variable without examining the directionality of effects. The present study examines the direction of effects between two domains of SPA (personal growth and physical losses) and processing speed (PS). Methods: The sample consists of 8,198 participants of the German Ageing Survey (DEAS), aged between 40 and 93 years. A cross-lagged path model was estimated to examine directions of relationships across 3 years via chi-squared difference tests for each domain of SPA. Results: In the unconstrained models, the effect of SPA domain personal growth in 2008 on PS in 2011 and vice versa were marginally significant. For SPA domain physical losses, the effect of SPA on PS was significant, whereas the other direction of the effect did not reach significance. However, the cross-lagged paths of both domains of SPA on PS and vice versa could be set equal without a significant loss of model fit. The resulting associations indicate a significant bidirectional relationship between both domains of SPA and PS. Discussion and conclusion: This study provides initial evidence that SPA can influence trajectories of cognitive decline and vice versa. The results emphasize the detrimental and beneficial effects that stereotypes can have on individuals and add further evidence to the theory of stereotype embodiment.

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