scholarly journals Structural Characteristics of Developing Nitella Internodal Cell Walls

1958 ◽  
Vol 4 (5) ◽  
pp. 505-516 ◽  
Author(s):  
Paul B. Green

The Nilella intermodal cell is formed by a division of the segment cell, the latter being a direct derivative of the shoot apical cell. The internodal cell is remarkable in that it elongates from an initial length of about 20 microns to a mature length of about 60 millimeters. The structures of the apical and segment cells, and the internodal cells in all stages of development were examined with the techniques of interference, polarization, and electron microscopy. The apical and segment cells were found to be isotropic. The upper part of the segment cell, destined to form a node, shows a curious pitted structure that was characteristic of certain node structures. The lower part of the segment cell, destined to become an internodal cell, shows a vague transverse arrangement of fibrils at the inner wall surface. The internodal cells, from the time they are first formed, show negative birefringence and a transverse arrangement of microfibrils at the inner wall surface. The elongation of the internodal cell is characterized by a rise, dip, and rise in both the optical thickness and retardation of the cell wall. The dip in both these variables coincides with the attainment of the maximum relative elongation rate. After the cessation of elongation, wall deposition continues, but the fibrils at .the inner surface of the wall are now seen to occur in fields of nearly parallel microfibrils. These fields, with varying fibrillar directions, may partly overlap each other or may merge with one another. Unlike the growing wall, this wall which is deposited after the end of elongation is isotropic.

2002 ◽  
Vol 726 ◽  
Author(s):  
Yongsoon Shin ◽  
Jun Liu ◽  
Li-Qiong Wang ◽  
Jeong Ho Chang ◽  
William D. Samuels ◽  
...  

AbstractWe here report the synthesis of ordered ceramic materials with hierarchy produced by an in-situ mineralization of ordered wood cellular structures with surfactant-templated sol-gel at different pH. At low pH, a silicic acid is coated onto inner surface of wood cellular structure and it penetrates into pores left, where degraded lignin and hemicellulose are leached out, to form a positive replica, while at high pH the precipitating silica particles due to fast condensation clog the cells and pit structures to form a negative replica of wood. The calcined monoliths produced in different pHs contain ordered wood cellular structures, multi-layered cell walls, pits, vessels well-preserved with positive or negative contrasts, respectively. The surfactant-templated mineralization produces ordered hexagonal nanopores with 20Å in the cell walls after calcination.


2020 ◽  
Vol 9 (5) ◽  
pp. 214-221
Author(s):  
H. Bokhari-Taieb Brahimi ◽  
D. E. Aizi ◽  
A. Bouhafsoun ◽  
K. Hachem ◽  
R. Mezemaze ◽  
...  

Retama monosperma is a fabaceous shrub that colonizes dune sands owing to its particularly important root system at depth and on the surface. It establishes symbiotic associations with rhizobia and thus plays a role in the bio -fertilization of soils. The stem fibers of R. monosperma are an interesting material for industry because of their useful biometric, physical, chemical and structural characteristics. The aim of this study was to complete these data with a biochemical analysis of the cell walls tissues of adult branches of R. monosperma. Cellulose, hemicelluloses and pectins were extracted from cell wall. The weight dosage indicated that cellulose remained the major component of the wall (56% from the crude cell wall and 52% from the delignified cell wall) ahead of hemicelluloses (16% from the crude cell wall and 14% from the delignified cell wall) and pectins (5.6% from the crude cell wall and 5% from the delignified cell wall for water extract pectins and 3% from the crude cell wall and 2.4% from the delignified cell wall for oxalate extract pectins). The colorimetric assay of pectins extracted from lignified cell wall of R. monosperma suggested presence of more uronic acids (14.95µg/mL) than pectins extracted from a delignified cell wall (12.37 µg/mL). Gas chromatographic analysis of hemicellulosic extracts showed the presence of xylose as the major ose (54.7% from the crude cell wall and 46.7% from the delignified cell wall). Pectins were represented by homogalacturonan chains and rhamnogalacturonans 1. Data generated in this study are helpful for valorization of this plant.


1963 ◽  
Vol 16 (4) ◽  
pp. 768 ◽  
Author(s):  
MS Buttrose

The developing aleurone layer cells of the wheat kernel have been investigated by electron microscopy and the results compared with those of light microscopy. Two weeks after flowering vacuoles appear in the cells and deposits accumulate in these until maturity when the cells are filled 'with the resulting "vacuolar units" 2-3p. in diameter, corresponding to the aleurone grains of light microscopy. The wheat aleurone grain consists of a bounding membrane (of vacuole origin) enclosing a matrix in which are embedded spherical deposits. Some of these deposits are translucent and others opaque to electrons after potassium permanganate and osmium tetroxide fixation. At all stages examined the cytoplasm of aleurone cells contained large numbers of small unidentified bodies with irregular outline and dense contents. At first they are dispersed, but towards maturity are organized as a monolayer over the surface of each aleurone grain and the inner surface of the cell walls. The apparent specificity of these structures to aleurone cells is discussed in relation to future chemical and physiological studies of the tissue.


2001 ◽  
Vol 44 (4) ◽  
pp. 405-410 ◽  
Author(s):  
Maria das Graças Sajo ◽  
Silvia Rodrigues Machado

The leaf ultrastructure of five Xyris species were examined using scanning electron microscope (SEM), transmission electron microscope (TEM) and histochemical methods. All studied leaves show some features in epidermis and mesophyll, which were of considerable adaptative significance to drought stress. Such features included the occurrence of a pectic layer on the stomatal guard cells and the presence of a network of pectic compounds in the cuticle. Pectic compunds were also in abundance in lamellated walls of the mesophyll cells and on the inner surface of the sclerified cell walls of the vascular bundle sheaths. There were also specialized chlorenchymatous "peg cells" in the mesophyll and drops of phenolic compounds inside the epidermal cells.


2016 ◽  
Vol 37 (4) ◽  
pp. 2043
Author(s):  
Wilton Ladeira Silva ◽  
Ana Cláudia Ruggieri ◽  
Ricardo Andrade Reis ◽  
Américo Garcia Silva Sobrinho ◽  
Euclides Braga Malheiros

The ideal time to start grazing is when pastures reach 95% light interception. The use of residual leaf area index (rLAI) to time the interruption of grazing under intermittent grazing has recently been studied in forage species in different climatic conditions in Brazil. However, studies evaluating the formation and development of leaves and tillers through morphogenetic and structural variables in functional rLAI are still lacking for Tifton 85 pastures. Therefore, the objective of this study was to evaluate the effects of varying rLAI in successive grazing cycles on morphogenetic and structural characteristics of Tifton 85 pasture grazed intermittently by sheep. Morphogenetic and structural characteristics were evaluated in three grazing cycles under three rLAI conditions (0.8, 1.6, and 2.4). The regrowth interval (time taken to return to 95% light interception) of pastures increased from 21.33 to 29.66 days with decreasing rLAI. The leaf appearance rate increased at a rate of 0.02 leaves tiller-1 day-1 and the number of live leaves per tiller increased by 0.41 with decreasing rLAI. Phyllochron, leaf elongation rate, final leaf length, leaf senescence rate, and stem elongation rate decreased linearly with decreasing rLAI. The number of live leaves per tiller was lower (7.97) in the third grazing cycle, probably owing to the higher senescence rate observed that same cycle, while the other variables had higher values. The 2.4 rLAI condition promotes excessive stem elongation, reduced leaf appearance, lower number of live leaves per tiller, and increased senescence of leaves, while the 0.8 and 1.6 rLAI conditions promote desirable morphogenetic and structural characteristics, which correlate directly with forage quality. In order to avoid excessive forage losses, grazing must occur preferentially by the end of March, when climatic conditions still support the appropriate development of the plants.


2017 ◽  
Vol 38 (5) ◽  
pp. 3271
Author(s):  
Márcio Odilon Dias Rodrigues ◽  
Antonio Clementino dos Santos ◽  
Marcos Odilon Dias Rodrigues ◽  
Rubens Ribeiro da Silva ◽  
Otacilio Silveira Junior

This study aimed to evaluate the effect of nitrogen (N) fertilizer doses on morphogenic and structural characteristics of mombassa grass (Panicum maximum Jacq.) at three different periods of the lifespan of tillers. The experiment was conducted in Araguaína-TO, Brazil, from December 2014 to February 2015. It was installed in split plots in a randomized block design with four replications. The doses of N-fertilizer (0, 30, 60, 90 kg N.ha-1) were the plots and the periods evaluated (1PER – first period from day 16 to 30; 2PER from day 31 to 45 and 3PER from day 46 to 60 after germination) were the subplots. The evaluated variables consisted of leaf appearance rate (LAR), leaf elongation rate (LER), stem elongation rate (SER), leaf senescence rate (LSR), phyllochron, tiller number, leaf emergence, and total leaf number. A Pearson correlation analysis was used to morphogenic and structural characteristics along with a regression analysis for all the variables. Overall, increasing N-fertilizer doses positively influenced the leaf appearance and leaf elongation rates, tiller density, and the number of live leaves per tiller. The 90-kg N.ha-1 dose enabled the best morphogenic and structural responses of the forage when compared to the other rates. Each period featured differently, with higher values of LSR, SER, and LER in 3PER when compared to those in 2PER and in 1PER. Therefore, the nutritional supply over the first weeks of tiller lives was defining to an increase in forage production in the remaining periods. In light of this, we observed that as LAR raise in the first days after germination, nutritional supplies are required from day 15 on, mainly nitrogen, so plants could continue their development since initial plant growth determines pasture productive vigor and longevity.


2017 ◽  
Vol 38 (1) ◽  
pp. 355
Author(s):  
Jennifer Figueiredo da Silva ◽  
Alan Da Cunha Honorato ◽  
Mário Adriano Ávila Queiroz ◽  
Mateus Matiuzzi da Costa ◽  
Adriana Mayumi Yano-Melo

The aim of this study was to evaluate the morphogenic, structural, and productive characteristics of Cenchrus ciliaris L. in response to different doses of biofertilizers that originate from the anaerobic biodigestion of goat and sheep fecal matter on Yellow Argisoil. The experiment was performed in a greenhouse, in a completely randomized factorial design using two types of biofertilizers and five dosages (0%, 2.5%, 5.0%, 7.5%, and 10% of soil volume), with four repeats, for the duration of two vegetative cycles. In the first cycle, there was an increase in leaf lifespan and leaf/stem ratio with the use of 10% and 7.5% caprine biofertilizer, respectively. From the second cycle, doses of biofertilizers between 7.5% and 10% led to an improvement in the development of the morphogenic variables - leaf sprouting rate, leaf and pseudostem elongation rate, leaf lifespan, and senescence, as well as the structural characteristics - expanded leaf length and width, number of sprouting, expanded and live leaves, and expanded/expanding leaf ratio, resulting in a 73% increase in biomass. It was concluded that the use of biofertilizers improves the main morphogenic and structural characteristics of Cenchrus ciliaris L., resulting in increased fodder mass yield.


2004 ◽  
Vol 82 (8) ◽  
pp. 1074-1088 ◽  
Author(s):  
R. Larry Peterson ◽  
Hugues B Massicotte

The roots or other subterranean organs of most plants develop symbioses, mycorrhizas, with fungal symbionts. Historically, mycorrhizas have been placed into seven categories based primarily on structural characteristics. A new category has been proposed for symbiotic associations of some leafy liverworts. An important feature of mycorrhizas is the interface involved in nutrient exchange between the symbionts. With the exception of ectomycorrhizas, in which fungal hyphae remain external to plant cell walls, all mycorrhizas are characterized by fungal hyphae breaching cell walls but remaining separated from the cell cytoplasm by a plant-derived membrane and an interfacial matrix that forms an apoplastic compartment. The chemical composition of the interfacial matrix varies in complexity. In arbuscular mycorrhizas (both Arum-type and Paris-type), molecules typical of plant primary cell walls (i.e., cellulose, pectins, β-1,3-glucans, hydroxyproline-rich glycoproteins) are present. In ericoid mycorrhizas, only rhamnogalacturonans occur in the interfacial matrix surrounding intracellular hyphal complexes. The matrix around intracellular hyphal complexes in orchid mycorrhizas lacks plant cell wall compounds until hyphae begin to senesce, then molecules similar to those found in primary cell walls are deposited. The interfacial matrix has not been studied in arbutoid mycorrhizas and ectendomycorrhizas. In ectomycorrhizas, the apoplastic interface consists of plant cell wall and fungal cell wall; alterations in these may enhance nutrient transfer. In all mycorrhizas, nutrients must pass into the symplast of both partners at some point, and therefore current research is exploring the nature of the opposing membranes, particularly in relation to phosphorus and sugar transporters.Key words: interface, apoplastic compartment, Hartig net, arbuscule, intracellular complex, nutrient exchange.


Sensors ◽  
2021 ◽  
Vol 21 (23) ◽  
pp. 8134
Author(s):  
Min Yao ◽  
Guangdong Luo ◽  
Min Zhao ◽  
Ruipeng Guo ◽  
Jian Liu

Only a few effective methods can detect internal defects and monitor the internal state of complex structural parts. On the basis of the principle of PET (positron emission computed tomography), a new measurement method, using γ photon to detect defects of an inner surface, is proposed. This method has the characteristics of strong penetration, anti-corrosion and anti-interference. With the aim of improving detection accuracy and imaging speed, this study also proposes image reconstruction algorithms, combining the classic FBP (filtered back projection) with MLEM (maximum likelihood expectation Maximization) algorithm. The proposed scheme can reduce the number of iterations required, when imaging, to achieve the same image quality. According to the operational demands of FPGAs (field-programmable gate array), a BPML (back projection maximum likelihood) algorithm is adapted to the structural characteristics of an FPGA, which makes it feasible to test the proposed algorithms therein. Furthermore, edge detection and defect recognition are conducted after reconstructing the inner image. The effectiveness and superiority of the algorithm are verified, and the performance of the FPGA is evaluated by the experiments.


2016 ◽  
Vol 37 (4) ◽  
pp. 2099
Author(s):  
Priscila Beligoli Fernandes ◽  
Carlos Augusto Brandão de Carvalho ◽  
Domingos Sávio Campos Paciullo ◽  
Carlos Augusto De Miranda Gomide ◽  
Mirton José Frota Morenz ◽  
...  

This work was carried out to evaluate the morphogenetic and structural characteristics of basal and aerial tillers of two dwarf elephant grass clones (BRS Kurumi and CNPGL 00-1-3) managed under intermittent stocking in six grazing cycles. A completely randomized experimental design with three replications (paddocks) in split-split-plot arrangements was used. The clones were allocated to the plots, tiller classes were assigned to the subplots and grazing cycles to sub subplots. Higher values for basal tillers than aerial tillers were seen for the following characteristics: leaf elongation rate (12.8 and 5.1 cm/tiller/day), leaf appearance rate (0.26 and 0.19 leaves/tiller/day), stem elongation rate (0.38 and 0.16 cm/tiller/day), senescence rate (0.98 and 0.47 cm/tiller/day), total number of leaves (9.3 and 7.1 leaves/ tiller), number of live leaves (7.7 and 5.8 leaves/tiller) and final length of leaf blades (33.5 and 20.0 cm). Phyllochron was lower for basal (4.0 days/leaf) than aerial tillers (5.5 days/leaf). The leaf life span increased with the advance of the grazing cycles, averaging 31.2 days. The tiller density increased with the advance of the grazing cycles showing an average increase of 167% of aerial tillers and 62% of basal tillers for both clones. The morphogenetic and structural characteristics of dwarf clones were influenced jointly by the clones and the availability of the environmental factors of growth during the spring and summer. The high leaf elongation rates, associated with high leaf appearance rate, reveal the potential of high production dry matter of leaves and restoration of leaf area after grazing.


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